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Follicle Stimulating Hormone, Human Recombinant
Catalog #: 228-12609
Product Description
Specifications
| Size | 10 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P01225 |
| Gene Id | 2488 |
| Protein Name / Synonyms | Follitropin subunit beta, Follicle-stimulating hormone beta subunit, FSH-beta, FSH-B, Follitropin beta chain, FSH. |
| Expressed Region | FSH subunit alpha: APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS. FSH subunit beta: NSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYETVRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEMKE. |
| Expression System | HEK293 cells |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The recombinant FSH was lyophilized from 0.2µm filtered solution containing PBS, pH 7.4. |
| Activity | ED50 determined by cAMP accumulation in human FSH Receptor transfected Chinese Hamster Ovary cells was found to be 80-450 pg/ml |
| Reconstitution | It is recommended to reconstitute the lyophilized Follicle Stimulating Hormone in sterile 18M(omega)-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| Research Area | Endocrine |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
FSH Human Recombinant produced in HEK-293 cells is heterodimeric, glycosylated, polypeptide chain transfected with two expression plasmids encoding the human FSH-alpha chain (Accession # P01215) (Ala25-Ser116) and human FSH-beta chain (Asn19-Glu129) (Accession # P01225) having a total Mw of 25kDa. FSH human recombinant is purified by proprietary chromatographic techniques.
Stability & Storage
Storage/Stability
Lyophilized recombinant FSH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FSH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.