GlycoRNAs are small non-coding RNAs with sialylated glycans and were recently discovered in 2021 by a Nobel laureate Chemical Biologist Dr. Carolyn Bertozzi. GlycoRNAs include small nuclear RNAs (snRNAs), ribosomal RNAs (rRNAs), small nucleolar RNAs (snoRNAs), transfer RNAs (tRNAs), and Y-RNAs.

RNA modifications (e.g., glycosylation, pseudouridylation, methylation, and adenylation) play pivotal roles in diverse cellular processes, such as tRNA-mediated translation, RNA epigenetics, chromatin structure modulation, and RNA maturation. Found primarily on the cell surface, glycoRNAs can participate in the immune system and cell-to-cell communication. In addition, glycoRNAs enhance the recruitment of neutrophils to inflammatory sites through their interaction with P-selectin on endothelial cells, and their expression and function are dependent on SIDT family genes. Despite recent progress in the field, our understanding of glycoRNAs is still lacking compared to that of proteins, lipids, and glycans.

The RayBio^® GlycoRNA Dot Blot Assay Kit is a reliable, simple, and rapid method of quantifying the total glycoRNA level in RNA samples collected from humans, rats, mice and other species.

• Highly sensitive and specific
• Requires minimal sample preparation
• No specialty equipment required
• Suitable for high-throughput screening
• Compatible with various sample types and species
• ≥ 48 samples could be detected by 1 kit

• RNase-free tubes
• Heat blocker
• 10 cm plastic petri dish
• UV cross-linker
• Chemiluminescent imaging system

1. Sample preparation: Depending on the type of sample (RNA, plasma, or serum), different methods of extraction, dilution, and denaturation are required.
2. Sample loading and crosslinking: The sample is spotted onto a nitrocellulose membrane, air dried, and crosslinked with UV light twice.
3. Methylene blue staining: One line of the spotted sample replicates is cut off and stained with methylene blue buffer to serve as an internal control.
4. Blocking and antibody incubation: The membrane is blocked with blocking buffer and then incubated with corresponding antibody and HRP-conjugated secondary antibody.
5. Detection and imaging: The membrane is treated with a mixture of detection buffers C and D, which catalyzes a color development reaction. The chemiluminescent signal is captured by an imaging system.

The entire kit may be stored at -20°C to -80°C for up to 12 months from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.