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Growth Hormone Bovine Recombinant
Catalog #: 228-10528
Product Description
Specifications
| Size | 100 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Bovine (cow) |
| Accession Number | P01246 |
| Gene Id | 280804 |
| Protein Name / Synonyms | BGH, BST, rBGH, rBST, Bovine Somatotropin, Bovine GH, Growth hormone, GH1. |
| Expressed Region | afpamslsgl fanavlraqh lhqlaadtfk efertyipeg qrysiqntqv afcfsetmpa ptgkneaqqk sdlellrisl lliqswlgpl qflsrvftns lvfgtsdrvy eklkdleegi lalmreledg tprrgqilkq tydkfdtnmr sddallknyg llscfrkdlh ktetylrvmk crrfgeasca f |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The Bovine Growth Hormone protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 adjusted to pH-8. |
| Activity | The activity as determined by the PDFP13B9 cells stably transfected with rabbit GH receptors. Bovine GH is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD). |
| Reconstitution | It is recommended to reconstitute the lyophilized Bovine Growth Hormone in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Research Area | Endocrine |
| Protein Content | UV spectroscopy at 280 nm using the absorbency value of 0.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Growth Hormone Bovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.8 kDa. The GH Bovine Recombinant is purified by proprietary chromatographic techniques.
Specifications
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Bovine Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GH Bovine should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.