Bovine serum albumin (BSA) is a large, stable, and highly immunogenic protein with multiple reactive sites per molecule, making it ideal for conjugation with peptides and other antigens via amine- or carboxyl-reactive crosslinkers. As such, BSA is widely used as a carrier protein to enhance the immunogenicity of haptens and weak antigens for applications such as antibody production, ELISAs, immunoblots, and immunohistochemistry.

The RayBio® Hapten-BSA Conjugation Kit provides an optimized method for generating hapten-carrier conjugates for immunization and antibody production. This kit employs a carboxyl-reactive carbodiimide crosslinker, which forms stable bonds between exposed carboxyl and amino groups on peptides and proteins. BSA is pre-formulated in buffers compatible with carbodiimide chemistry and the use of spin desalting columns ensures exceptional protein recovery through a simple centrifugation step.

• Hapten such as DNA or RNA oligonucleotide, small molecule, peptide or small protein containing a primary amino group
• Centrifuge
• Sterile nuclease-free pipette tips (barrier tips recommended) and 1.5 mL microcentrifuge tubes
• 15 mL tubes
• Nuclease-free water
• PBS (optional)
• DMSO (optional)

Contact our technical support team for questions: techsupport@raybiotech.com

1. Resuspend one vial of BSA in nuclease-free water.
2. Dissolve hapten (up to 2 mg) in Conjugation Buffer.
3. Add the hapten solution to the BSA solution to make Hapten-BSA solution.
4. Resuspend one vial of EDC in the Hapten-BSA solution and mix gently.
5. Prepare the Spin Desalting Column by removing the storage solution and washing with Purification Buffer.
6. Apply the Hapten-BSA-EDC solution to the column and centrifuge to collect the conjugate.
7. Quantify the Hapten-BSA conjugate by BCA protein assay.
8. The Hapten-BSA conjugate can be used for immunization or stored for later use.

The entire kit may be stored at 2 - 8°C. Do not freeze. The kit can be stored for up to 12 months from the date of shipment. Minimize light exposure.