CFBE is a Cystic Fibrosis (CF) human bronchial epithelial cell line, derived from a CF patient homozygous for the ΔF508 CFTR mutation. CFBE cells can polarize and form tight junction. They demonstrate all ion transport properties characteristic of cystic fibrosis, such as defective cAMP-dependent chloride transport and intact calcium dependent chloride transport. The CFBE cell line subclones, WT-CFTR and ΔF508-CFTR, can be used to study the relationship between CFTR gene expression and chloride transport function.

CFBE-HLA-A stable cell line is transformed from the CFBE (ΔF508-CFTR) cell line and stably expresses the HLA-A:02:01 protein. The expression of HLA-A:02:01 has been validated by western blot.

• Minimum Essential Medium α (MEM α)
• Fetal Bovine Serum (FBS)
• Penicillin/Streptomycin
• Trypsin
• Phosphate-buffered saline (PBS)
• DMSO
• 96-well white plate

Store the frozen cells in liquid nitrogen until you are ready to thaw and propagate them. It is strongly recommended that you propagate the cells following instructions as soon as possible upon arrival. An adequate number of frozen stocks must be made from early passages as cells will undergo genotypic changes. Genetic instability in transfected cells will result in a decreased responsiveness over time in normal cell culture conditions.