Human ANGPT1 ELISA

RayBio® Human Angiopoietin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-Angiopoietin1-1
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Antigen Information

Accession Number:
  • Q15389
Gene ID:
  • 284
Gene Symbols:
  • ANGPT1
  • KIAA0003
Protein Name & Synonyms:
Angiopoietin-1 (ANG-1)
Target Species:
  • Human

Assay Format

Detection Range:
30 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
3 - 30 fold
Sensitivity:
30 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Tasaki, Yuko, et al. "Disruption of vascular endothelial homeostasis in systemic juvenile idiopathic arthritis-associated macrophage activation syndrome: The dynamic roles of angiopoietin-1 and-2." Cytokine 80 (2016): 1-6.
    Species: Human
    Sample type: Plasma
  2. Chen S., Li H., Zhang C., et al. Urinary Angiopoietin-2 Is Associated with Albuminuria in Patients with Type 2 Diabetes Mellitus. International Journal of Endocrinology Volume 2014, Article ID 163120, 7 pages .http://dx.doi.org/10.1155/2014/163120
    Species: Human
    Sample type: Urine
  3. Fernandez-Yunquera A., et al. Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients. World J Transplant 2014; 4(2): 133-140. http://dx.doi.org/10.5500/wjt.v4.i2.133
    Species: Human
    Sample type: Serum
  4. Koksal BT., Ozbek OY., Bayraktar N., et al. Evaluation of angiopoietin 1 and 2, vascular endothelial growth factor, and tumor necrosis factor alpha levels in asthmatic children. Allergy and Asthma Proceedings, Volume 35, Number 6, November/December 2014, pp. 482-488(7). http://dx.doi.org/10.2500/aap.2014.35.3793
    Species: Human
    Sample type: Serum
  5. Swamynatha P., et al. Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study. Stem Cell Research & Therapy 2014, 5:88 doi:10.1186/scrt477.
    Species: Human
    Sample type: Conditioned Media
  6. Ferrer RA, Wobus M, List C, et al. Mesenchymal stromal cells from patients with myelodyplastic syndromedisplay distinct functional alterations that are modulated by lenalidomide. Haematologica. 2013; Published online before print May 28, 2013, doi: 10.3324/haematol.2013.083972
    Species: Human
    Sample type: Conditioned Media
  7. Khan R, Sharma M, Kumar L, et al. Interrelationship and expression profiling of cyclooxygenase and angiogenic factors in Indian patients with multiple myeloma. Ann Hematol. 2013 Jan;92(1):101-109.
    Species: Human
    Sample type: Serum
  8. Palumbo S, Li W-J. Osteoprotegerin Enhances Osteogenesis of Human Mesenchymal Stem Cells. Tissue Eng Part A. Volume 19, Numbers 19 and 20, 2013, DOI: 10.1089/ten.tea.2012.0550
    Species: Human
    Sample type: Conditioned Media
  9. Synergistic effect of vascular endothelial growth factor and angiopoietin-2 on progression free survival in multiple myeloma
    Species: Human
    Sample type: Serum
  10. Vizio, Barbara, Fiorella Biasi, and Giuseppe Poli. "Pancreatic-carcinoma-cell-derived Pro-angiogenic Factors Can Induce Endothelial-cell Differentiation of a Subset of Circulating CD34+ Progenitors." Journal of Translational Medicine. NCBI, 17 Dec. 2013. Web.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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