Human ANGPTL4 ELISA

$398.00
ELH-ANGPTL4-1

RayBio® Human ANGPTL4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
20 pg/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
20 pg/ml - 20000 pg/ml
Recommended Dilution (serum & plasma)
3 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Species Detected
Human
Protein Name & Synonyms
Angiopoietin-related protein 4 (Angiopoietin-like protein 4) (Hepatic fibrinogen/angiopoietin-related protein) (HFARP)
Accession Number
Gene Names
ANGPTL4, ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197
Gene ID
51129

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • ANGPTL4

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
    Species: Human
    Sample Type: Plasma

  2. Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
    Species: Human
    Sample Type: Conditioned Media

  3. Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
    Species: Human
    Sample Type: Serum

  4. Basak S, Duttaroy AK. cis-9,trans-11 conjugated linoleic acid stimulates expression of angiopoietin like-4 in the placental extravillous trophoblast cells. Biochim Biophys Acta. 2013;1831(4):834-843.
    Species: Human
    Sample Type: Conditioned Media

  5. Clasen BFF, Krusenstjerna-Hafstrom T, Vendelbo MH, et al. Gene Expression in Skeletal Muscle after an Acute Intravenous GH Bolus in Human Subjects: Identification of a Mechanism Regulating ANGPTL4. J Lipid Res. 2013;57(7):1988-1997.
    Species: Human
    Sample Type: Serum

  6. Drager LF, Yao Q, Hernandez KL, et al. Chronic Intermittent Hypoxia Induces Atherosclerosis via Activation of Adipose Angiopoietin-like 4. Am. J. Respir. Crit. Care Med. 2013 Jan 17;
    Species: Human
    Sample Type: Serum

  7. Baranowski, T., et al. "Serum levels of the adipokine fasting-induced adipose factor/angiopoietin-like protein 4 depend on renal function." Hormone and metabolic research 43.02 (2011): 117-120.

Write a review

No posts found

U.S. International

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Cart content Cart content