Human BDNF ELISA

$398.00
ELH-BDNF-1

RayBio® Human BDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
80 pg/ml

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Detection Range
80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma)
5 - 500 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Brain-derived neurotrophic factor (BDNF) (Abrineurin)
Accession Number
Gene Names
BDNF
Gene ID
627

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • BDNF

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Failla M., Juengst S., Arenth P., Wagner A. Preliminary Associations Between Brain-Derived Neurotrophic Factor, Memory Impairment, Functional Cognition, and Depressive Symptoms Following Severe TBI. Neurorehabil Neural Repair. 2016 Jun;30(5):419-30. doi: 10.1177/1545968315600525.
    Species: Human
    Sample Type: Serum

  2. Failla M., Juengst S., Arenth P., Wagner A. Preliminary Associations Between Brain-Derived Neurotrophic Factor, Memory Impairment, Functional Cognition, and Depressive Symptoms Following Severe TBI. Neurorehabil Neural Repair. 2016 Jun;30(5):419-30. doi: 10.1177/1545968315600525.
    Species: Human
    Sample Type: CSF

  3. Sadowska-Krepa E., Zwierzchowska A., Glowacz M., et al. Blood metabolic response to a long-term wheelchair rugby training. Spinal Cord. 2016 May;54(5):371-5. doi: 10.1038/sc.2015.178.
    Species: Human
    Sample Type: Serum

  4. Wysokinski A., Serum levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in depressed patients with schizophrenia. Nord J Psychiatry. 2016;70(4):267-71. doi: 10.3109/08039488.2015.1087592
    Species: Human
    Sample Type: Serum

  5. Shen C., Lie P., Miao T., et al. Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis. Mol Med Rep. 2015 Jul;12(1):20-30. doi: 10.3892/mmr.2015.3409
    Species: Human
    Sample Type: Conditioned Media

  6. Oral E., Kirkan TS., Yildirim A., et al. Serum brain-derived neurotrophic factor differences between the luteal and follicular phases in premenstrual dysphoric disorder. Gen Hosp Psychiatry. 2015 May-Jun;37(3):266-72. doi: 10.1016/j.genhosppsych.2015.03.001
    Species: Human
    Sample Type: Serum

  7. Lu H., Zhang T., Wen M., Sun L. Impact of Repetitive Transcranial Magnetic Stimulation on Post-Stroke Dysmnesia and the Role of BDNF Val66Met SNP. Med Sci Monit. 2015 Mar 14;21:761-8. doi: 10.12659/MSM.892337.
    Species: Human
    Sample Type: Plasma

  8. Jiang H., Ling Z., Zhang Y., et al. Altered fecal microbiota composition in patients with major depressive disorder. Brain Behav Immun. 2015 Aug;48:186-94. doi: 10.1016/j.bbi.2015.03.016
    Species: Human
    Sample Type: Serum

  9. Haslacher H., Michlmayr M., Batmyagmar D., et al. Physical exercise counteracts genetic susceptibility to depression. Neuropsychobiology. 2015;71(3):168-75. doi: 10.1159/000381350.
    Species: Human
    Sample Type: Serum

  10. Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
    Species: Human
    Sample Type: Serum

  11. Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
    Species: Human
    Sample Type: CSF

  12. Eleftheriou D., Ganesan V., Hong Y., et al. Endothelial Repair in Childhood Arterial Ischaemic Stroke with Cerebral Arteriopathy. Cerebrovasc Dis Extra. 2015 Jun 11;5(2):68-74. doi: 10.1159/000381963.
    Species: Human
    Sample Type: Plasma

  13. Zhang Y., Lin P., Jiang H., et al. Extensive serum biomarker analysis in patients with ST segment elevation myocardial infarction (STEMI). Cytokine. 2015 Dec;76(2):356-62. doi: 10.1016/j.cyto.2015.06.015.
    Species: Human
    Sample Type: Serum

  14. Kielstein H., Suntharalingam M., Perthel R., et al. Role of the endogenous nitric oxide inhibitor asymmetric dimethylarginine (ADMA) and brain-derived neurotrophic factor (BDNF) in depression and behavioural changes: clinical and preclinical data in chronic kidney disease. Nephrol Dial Transplant. 2015 Oct;30(10):1699-705. doi: 10.1093/ndt/gfv253
    Species: Human
    Sample Type: Serum

  15. Schendzielorz P., Rak K., Nguyen J., et al. Human adipose-derived stem cells enhance the survival and neuritogenesis of auditory neurons. Neuroreport. 2015 Sep 9;26(13):797-801. doi: 10.1097/WNR.0000000000000427.
    Species: Human
    Sample Type: Conditioned Media

  16. Faroni A., Smith R., Lu L., et al. Human Schwann-like cells derived from adipose-derived mesenchymal stem cells rapidly de-differentiate in the absence of stimulating medium. Eur J Neurosci. 2016 Feb;43(3):417-30. doi: 10.1111/ejn.13055.
    Species: Human
    Sample Type: Conditioned Media

  17. Li W., He Q., Wu C., et al. PFOS Disturbs BDNF-ERK-CREB Signalling in Association with Increased MicroRNA-22 in SH-SY5Y Cells. Biomed Res Int. 2015;2015:302653. doi: 10.1155/2015/302653
    Species: Human
    Sample Type: Conditioned Media

  18. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
    Species: Human
    Sample Type: Conditioned Media

  19. Cai M, Zhou Y, Zhou B, Lou S (2014) Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation ofNeural Stem Cells Mainly through PI3-K/Akt Pathways. PLoS ONE 9(11): e111938. doi:10.1371/journal.pone.0111938
    Species: Mouse
    Sample Type: Conditioned Media

  20. Fornaro M., Escelsior A., Rocchi G., et al. BDNF plasma levels variations in major depressed patients receiving duloxetine. Neurol Sci, 2014 epub ahead of print. DOI 10.1007/s10072-014-2027-7
    Species: Human
    Sample Type: Serum

  21. Liu Y., et al. Associations Between ApoEε4 Carrier Status and Serum BDNFLevels—New Insights into the Molecular Mechanism of ApoEε4. Mol Neurobio. Online First June 2014. DOI 10.1007/s12035-014-8804-8Actions in Alzheimer’s Disease
    Species: Human
    Sample Type: Serum

  22. Sahin S., et al. Effect of Methylphenidate Treatment on Appetite and Levels of Leptin, Ghrelin, Adiponectin, and Brain-Derived Neurotrophic Factor in Children and Adolescents with Attention Deficit and Hyperactivity Disorder. International Journal of Pyschiatry in Clinical Practice. Posted online on July 4, 2014. (doi:10.3109/13651501.2014.940054)
    Species: Human
    Sample Type: Serum

  23. Sinkevicius K., et al. Neurotrophin receptor TrkB promotes lungadenocarcinoma metastasis. PNAS 2014 111 (28) 10299-10304; published ahead of print June 30, 2014, doi:10.1073/pnas.1404399111
    Species: Human
    Sample Type: Conditioned Media

  24. Oral E, Ozcan H, Kirkan TS, et al. Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder. Eur Arch Psychiatry Clin Neurosci. 2013 Mar 2; [Epub ahead of print] doi:10.1007/s00406-013-0398-z.
    Species: Human
    Sample Type: Serum

  25. Pompeia S, Gouveia JR, Galduróz JCF. Acute mood effect of donepezil in young, healthy volunteers. Hum Psychopharmacol. 2013 May;28(3):263–269.
    Species: Human
    Sample Type: Plasma

  26. Uguz F, Sonmez EO, Sahingoz M, et al. Maternal generalized anxiety disorder during pregnancy and fetal brain development: A comparative study on cord blood brain-derived neurotrophic factor levels. J Psychosomatic Res. 2013 Aug 5; [Epub ahead of print] doi:10.1016/j.jpsychores.2013.07.002.
    Species: Human
    Sample Type: Serum

  27. Browne AS., et al. Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis. Fertil Steril. 2012 Sep;98(3):713-9. doi: 10.1016/j.fertnstert.2012.05.027.
    Species: Human
    Sample Type: Tissue Lysate

  28. Browne, Aimee S. et al. Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis. Fertility and Sterility , Volume 98 , Issue 3 , 713 - 721
    Species: Human
    Sample Type: Tissue Lysate

  29. Oral E., Canpolat S., Yildiirim S., et al. Cognitive functions and serum levels of brain-derived neurotrophic factor in patients with major depressive disorder. Brain Res Bull. 2012 Aug 1;88(5):454-9. doi: 10.1016/j.brainresbull.2012.03.005.
    Species: Human
    Sample Type: Serum

  30. Corripio R., Gonzalez-Celmente J., Jacobo P., et al. Plasma brain-derived neurotrophic factor in prepubertal obese children: results from a 2-year lifestyle intervention programme. Clin Endocrinol (Oxf). 2012 Nov;77(5):715-20. doi: 10.1111/j.1365-2265.2012.04431.x.
    Species: Human
    Sample Type: Plasma

  31. Klein K, Aeschlimann A, Jordan S, Gay R, Gay S, et al. (2012) ATP Induced Brain-Derived Neurotrophic Factor Expression and Release from Osteoarthritis Synovial Fibroblasts Is Mediated by Purinergic Receptor P2X4. PLoS ONE 7(5): e36693. doi:10.1371/journal.pone.0036693
    Species: Human
    Sample Type: Conditioned Media

  32. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 – 1883
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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