Human beta-NGF ELISA

RayBio® Human beta-NGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-BNGF-1
+

Antigen Information

Accession Number:
  • P01138
Gene ID:
  • 4803
Gene Symbols:
  • NGF
  • NGFB
Protein Name & Synonyms:
Beta-nerve growth factor (Beta-NGF)
Target Species:
  • Human

Assay Format

Detection Range:
14 pg/ml - 5000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
14 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ciftci S., Ozkurkcugil C., Yilmaz H., et al. Urinary nerve growth factor and a variable solifenacin dosage in patients with an overactive bladder. Int Urogynecol J. 2016 Feb;27(2):275-80. doi: 10.1007/s00192-015-2825-3.
    Species: Human
    Sample type: Urine
  2. Faroni A., Smith R., Lu L., et al. Human Schwann-like cells derived from adipose-derived mesenchymal stem cells rapidly de-differentiate in the absence of stimulating medium. Eur J Neurosci. 2016 Feb;43(3):417-30. doi: 10.1111/ejn.13055.
    Species: Human
    Sample type: Conditioned Media
  3. Krock E., Currie J., Weber M., et al. Nerve Growth Factor Is Regulated by Toll-Like Receptor 2 in Human Intervertebral Discs. J Biol Chem. 2016 Feb 12;291(7):3541-51. doi: 10.1074/jbc.M115.675900
    Species: Human
    Sample type: Conditioned Media
  4. Sun J., Mandai M., Kamao H., et al. Protective Effects of Human iPS-Derived Retinal Pigmented Epithelial Cells in Comparison with Human Mesenchymal Stromal Cells and Human Neural Stem Cells on the Degenerating Retina in rd1 mice. Stem Cells. 2015 May;33(5):1543-53. doi: 10.1002/stem.1960.
    Species: Human
    Sample type: Conditioned Media
  5. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
    Species: Human
    Sample type: Conditioned Media
  6. Lambiase, Alessandro, Mauro P. Ferrari, and Flavio Mantelli. "Safety and Pharmacokinetics of Escalating Doses of Human Recombinant Nerve Growth Factor Eye Drops in a Double-Masked, Randomized Clinical Trial." BioDrugs. Springer Link, Dec. 2013. Web.
    Species: Human
    Sample type: Serum
  7. Jalali, H., Parivar, K., Nabiuni, M., & Soleimani, M. (2013). Unrestricted somatic stem cells as vehicle for nerve growth factor gene transfer. Neurological research, 35(6), 553-560.
    Species: Human
    Sample type: Cell Lysate
  8. Kalinowska-Lyszczarz A., Pawlak M., Michalak S., Losy J. Cognitive deficit is related to immune-cell beta-NGF in multiple sclerosis patients. J Neurol Sci. 2012 Oct 15;321(1-2):43-8. doi: 10.1016/j.jns.2012.07.044
    Species: Human
    Sample type: Cell Lysate
  9. Kalinowska-lyszczarz A., et al. Immune cell NT-3 expression is associated with brain atrophy in multiple sclerosis patients. J Neuroimmunol. 2011 Dec 15;240-241:109-13. doi: 10.1016/j.jneuroim.2011.10.002.
    Species: Human
    Sample type: Serum
  10. Reid, Adam J., et al. "Nerve repair with adipose-derived stem cells protects dorsal root ganglia neurons from apoptosis." Neuroscience 199 (2011): 515-522.
    Species: Human
    Sample type: Conditioned Media
The product was user friendly. As a first time student conducting an ELISA, the directions were workable. The direction paper has everything you need, plus example dilutions. The standards were also easy to understand. Will get this kit again when I need to analyze NGF!

University of Northern Colorado
This kit worked great for detecting NGF in human serum and plasma samples. Like all their other kits it was very easy to use and straight forward. The suggested dilution of the kit is 2:1 for human samples. However, I suggest, if you have sample to spare, to run the serum or plasma straight to avoid falling near the bottom of the standard curve.

University of Northern Colorado
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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