Human beta-NGF ELISA

$398.00
ELH-BNGF-1

RayBio® Human beta-NGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
14 pg/ml

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Detection Range
14 pg/ml - 5000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Species Detected
Human
Protein Name & Synonyms
Beta-nerve growth factor (Beta-NGF)
Accession Number
Gene Names
NGF, NGFB
Gene ID
4803

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • NGF

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Ciftci S., Ozkurkcugil C., Yilmaz H., et al. Urinary nerve growth factor and a variable solifenacin dosage in patients with an overactive bladder. Int Urogynecol J. 2016 Feb;27(2):275-80. doi: 10.1007/s00192-015-2825-3.
    Species: Human
    Sample Type: Urine

  2. Faroni A., Smith R., Lu L., et al. Human Schwann-like cells derived from adipose-derived mesenchymal stem cells rapidly de-differentiate in the absence of stimulating medium. Eur J Neurosci. 2016 Feb;43(3):417-30. doi: 10.1111/ejn.13055.
    Species: Human
    Sample Type: Conditioned Media

  3. Krock E., Currie J., Weber M., et al. Nerve Growth Factor Is Regulated by Toll-Like Receptor 2 in Human Intervertebral Discs. J Biol Chem. 2016 Feb 12;291(7):3541-51. doi: 10.1074/jbc.M115.675900

  4. Sun J., Mandai M., Kamao H., et al. Protective Effects of Human iPS-Derived Retinal Pigmented Epithelial Cells in Comparison with Human Mesenchymal Stromal Cells and Human Neural Stem Cells on the Degenerating Retina in rd1 mice. Stem Cells. 2015 May;33(5):1543-53. doi: 10.1002/stem.1960.
    Species: Human
    Sample Type: Conditioned Media

  5. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
    Species: Human
    Sample Type: Conditioned Media

  6. Lambiase, Alessandro, Mauro P. Ferrari, and Flavio Mantelli. "Safety and Pharmacokinetics of Escalating Doses of Human Recombinant Nerve Growth Factor Eye Drops in a Double-Masked, Randomized Clinical Trial." BioDrugs. Springer Link, Dec. 2013. Web.
    Species: Human
    Sample Type: Serum

  7. Kalinowska-Lyszczarz A., Pawlak M., Michalak S., Losy J. Cognitive deficit is related to immune-cell beta-NGF in multiple sclerosis patients. J Neurol Sci. 2012 Oct 15;321(1-2):43-8. doi: 10.1016/j.jns.2012.07.044
    Species: Human
    Sample Type: Serum

  8. Kalinowska-Lyszczarz A., Pawlak M., Michalak S., Losy J. Cognitive deficit is related to immune-cell beta-NGF in multiple sclerosis patients. J Neurol Sci. 2012 Oct 15;321(1-2):43-8. doi: 10.1016/j.jns.2012.07.044
    Species: Human
    Sample Type: Cell Lysate

  9. Kalinowska-lyszczarz A., et al. Immune cell NT-3 expression is associated with brain atrophy in multiple sclerosis patients. J Neuroimmunol. 2011 Dec 15;240-241:109-13. doi: 10.1016/j.jneuroim.2011.10.002.
    Species: Human
    Sample Type: Cell Lysate

  10. Kalinowska-lyszczarz A., et al. Immune cell NT-3 expression is associated with brain atrophy in multiple sclerosis patients. J Neuroimmunol. 2011 Dec 15;240-241:109-13. doi: 10.1016/j.jneuroim.2011.10.002.
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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