Human bFGF ELISA

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RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-bFGF-1
+

Antigen Information

Accession Number:
  • P09038
Gene ID:
  • 2247
Gene Symbols:
  • FGF2
  • FGFB
Protein Name & Synonyms:
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Target Species:
  • Human

Assay Format

Detection Range:
50 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
50 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Hironori Y., Yoshihisa H., Hisayuki K.
    Species: Human
    Sample type: Conditioned Media
  2. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample type: Conditioned Media
  3. Sowmya S., Chennazhi K., Arzate H., et al. Periodontal Specific Differentiation of Dental Follicle Stem Cells into Osteoblast, Fibroblast, and Cementoblast. Tissue Eng Part C Methods. 2015 Oct;21(10):1044-58. doi: 10.1089/ten.TEC.2014.0603.
    Species: Human
    Sample type: Conditioned Media
  4. Guan P., Guo J., Yu X., et al. The role of cyclooxygenase-2, interleukin-1? and fibroblast growth factor-2 in the activation of matrix metalloproteinase-1 in sheared-chondrocytes and articular cartilage. Sci Rep. 2015 May 20;5:10412. doi: 10.1038/srep10412.
    Species: Human
    Sample type: Conditioned Media
  5. Aly Fouad N., Khodair S., Farweez B., et al. Prognostic Significance of VEGF and bFGF in Egyptian CLL Patients. Journal of Medical Sceince and Clinical Research Volume 3 Issue 09 Sep 2015
    Species: Human
    Sample type: Serum
  6. Al-Shammari A. Allak W., Umran M., et al. Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured in Vitro. Advances in Breast Cancer Research, 2015, 4, 100-108, http://dx.doi.org/10.4236/abcr.2015.44011
    Species: Human
    Sample type: Conditioned Media
  7. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  8. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample type: Tissue Lysate
  9. Koob TJ., Lim JJ., Massee M., Zabek N., Denoziere G. Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation. J Biomed Mater Res B Appl Biomater. 2014 Mar 25 [Epub ahead of print]
    Species: Human
    Sample type: Tissue Lysate
  10. Lai H., Kuan C., Wu H., Tsai J., Chen T., Hsieh D., Wang T. Tailor Design Electrospun Composite Nanofibers with Staged Release of Multiple Angiogenic Growth Factors for Chronic Wound Healing. Acta Biomaterialia Available online May 9, 2014. http://dx.doi.org.proxy.library.emory.edu/10.1016/j.actbio.2014.05.001 [Epub ahead of print]
    Species: Human
    Sample type: Nanomaterial

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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