Human bFGF ELISA

$398.00
ELH-bFGF-1

RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
50 pg/ml

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Detection Range
50 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Accession Number
Gene Names
FGF2, FGFB
Gene ID
2247

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • FGF2

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Hironori Y., Yoshihisa H., Hisayuki K.
    Species: Human
    Sample Type: Conditioned Media

  2. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample Type: Conditioned Media

  3. Sowmya S., Chennazhi K., Arzate H., et al. Periodontal Specific Differentiation of Dental Follicle Stem Cells into Osteoblast, Fibroblast, and Cementoblast. Tissue Eng Part C Methods. 2015 Oct;21(10):1044-58. doi: 10.1089/ten.TEC.2014.0603.
    Species: Human
    Sample Type: Conditioned Media

  4. Guan P., Guo J., Yu X., et al. The role of cyclooxygenase-2, interleukin-1β and fibroblast growth factor-2 in the activation of matrix metalloproteinase-1 in sheared-chondrocytes and articular cartilage. Sci Rep. 2015 May 20;5:10412. doi: 10.1038/srep10412.
    Species: Human
    Sample Type: Conditioned Media

  5. Aly Fouad N., Khodair S., Farweez B., et al. Prognostic Significance of VEGF and bFGF in Egyptian CLL Patients. Journal of Medical Sceince and Clinical Research Volume 3 Issue 09 Sep 2015
    Species: Human
    Sample Type: Serum

  6. Al-Shammari A. Allak W., Umran M., et al. Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured in Vitro. Advances in Breast Cancer Research, 2015, 4, 100-108, http://dx.doi.org/10.4236/abcr.2015.44011
    Species: Human
    Sample Type: Conditioned Media

  7. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample Type: Tissue Lysate

  8. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample Type: Tissue Lysate

  9. Koob TJ., Lim JJ., Massee M., Zabek N., Denoziere G. Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation. J Biomed Mater Res B Appl Biomater. 2014 Mar 25 [Epub ahead of print]
    Species: Human
    Sample Type: Tissue Lysate

  10. Lai H., Kuan C., Wu H., Tsai J., Chen T., Hsieh D., Wang T. Tailor Design Electrospun Composite Nanofibers with Staged Release of Multiple Angiogenic Growth Factors for Chronic Wound Healing. Acta Biomaterialia Available online May 9, 2014. http://dx.doi.org.proxy.library.emory.edu/10.1016/j.actbio.2014.05.001 [Epub ahead of print]
    Species: Human
    Sample Type: Nanomaterial

  11. Synergistic effect of vascular endothelial growth factor and angiopoietin-2 on progression free survival in multiple myeloma
    Species: Human
    Sample Type: Serum

  12. Yalçındağ A, Gedik-Oğuz Y, Yalçındağ FN. The relationship between serum levels of angiogenin, bFGF, VEGF, and ocular involvement in patients with Behçet’s disease. Graefes Arch. Clin. Exp. Ophthalmol. 2013 Apr 4;
    Species: Human
    Sample Type: Serum

  13. Chim H., Armijo BS., Miller E., et al. Propranolol induces regression of hemangioma cells through HIF-1α-mediated inhibition of VEGF-A. Ann Surg. 2012 Jul;256(1):146-56. doi: 10.1097/SLA.0b013e318254ce7a.
    Species: Human
    Sample Type: Conditioned Media

  14. Fedak PW, Bai L., Turnbull J., et al. Cell therapy limits myofibroblast differentiation and structural cardiac remodeling: basic fibroblast growth factor-mediated paracrine mechanism. Circ Heart Fail. 2012 May 1;5(3):349-56. doi: 10.1161/CIRCHEARTFAILURE.111.965889
    Species: Human
    Sample Type: Tissue Lysate

  15. Kuhn MN., Willenberg HS., Schott M., et al. Adipocyte-secreted factors increase osteoblast proliferation and the OPG/RANKL ratio to influence osteoclast formation. Mol Cell Endocrinol. 2012 Feb 26;349(2):180-8. doi: 10.1016/j.mce.2011.10.018.
    Species: Human
    Sample Type: Conditioned Media

  16. Nazarov I, Lee JW, Soupene E, et al. Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. Stem Cells Translational Medicine. 2012;1(5):359-372. doi:10.5966/sctm.2011-0021.
    Species: Human
    Sample Type: Conditioned Media

  17. Brown K., Lau J., Dom A., et al. MG624, an α7-nAChR antagonist, inhibits angiogenesis via the Egr-1/FGF2 pathway. Angiogenesis. 2012 Mar;15(1):99-114. doi: 10.1007/s10456-011-9246-9.
    Species: Human
    Sample Type: Conditioned Media

  18. Saygun I., Nizam N., Ural A., et al. Low-level laser irradiation affects the release of basic fibroblast growth factor (bFGF), insulin-like growth factor-I (IGF-I), and receptor of IGF-I (IGFBP3) from osteoblasts. Photomed Laser Surg. 2012 Mar;30(3):149-54. doi: 10.1089/pho.2011.3079.
    Species: Human
    Sample Type: Conditioned Media

  19. Dragoo J., Korotkova T., Wasterlain A., et al. Age-Related Changes of Chondrogenic Growth Factors in Platelet-Rich Plasma. Operative Techniques in Orthopaedics, Volume 22, Issue 2, June 2012, Pages 49–55
    Species: Human
    Sample Type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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