Human BMP-4 ELISA

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RayBio® Human BMP-4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-BMP4-1
+

Antigen Information

Accession Number:
  • P12644
Gene ID:
  • 652
Gene Symbols:
  • BMP2B
  • BMP4
  • DVR4
Protein Name & Synonyms:
Bone morphogenetic protein 4 (BMP-4) (Bone morphogenetic protein 2B) (BMP-2B)
Target Species:
  • Human

Assay Format

Detection Range:
15 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
15 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, BMP-6, BMP-7, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GMCSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Bhattacharyya S. Feferman L., Tobacman JK. Regulation of chondroitin-4-sulfotransferase (CHST11) expression by opposing effects of arylsulfatase B on BMP4 and Wnt9A. Biochim Biophys Acta. 2015 Mar;1849(3):342-52. doi: 10.1016/j.bbagrm.2014.12.009
    Species: Human
    Sample type: Conditioned Media
  2. Pallotta I., Sun B., Wrona E., Freytes D. BMP protein-mediated crosstalk between inflammatory cells and human pluripotent stem cell-derived cardiomyocytes. J Tissue Eng Regen Med. 2015 Jun 23. doi: 10.1002/term.2045
    Species: Human
    Sample type: Conditioned Media
  3. Youn J., Zhou J, Cai H. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus. Mol Endocrinol. 2015 Aug;29(8):1123-33. doi: 10.1210/ME.2014-1313.
    Species: Mouse
    Sample type: Plasma
  4. Dincel VE., The importance and the differences of bone morphogenetic proteinsfor osteoporotic hip fractures. Acta Orthopædica Belgica, Vol. 80 - 2 - 2014
    Species: Human
    Sample type: Serum
  5. Zhang S., et al. Disordered hepcidin-ferroportin signaling promotes breast cancer growth. Cellular Signalling available online 3 August 2014. DOI: 10.1016/j.cellsig.2014.07.029
    Species: Human
    Sample type: Serum
  6. Lai D, Yang X. BMP4 is a novel transcriptional target and mediator of mammary cell migration downstream of the Hippo pathway component TAZ. Cell Signal. 2013 May 11;25(8):1720-1728.
    Species: Human
    Sample type: Conditioned Media
  7. Kanemura, Hoshimi, et al. "Pigment epithelium-derived factor secreted from retinal pigment epithelium facilitates apoptotic cell death of iPSC." Scientific reports 3 (2013): 2334.
    Species: Human
    Sample type: Conditioned Media
  8. Dragoo J., Korotkova T., Wasterlain A., et al. Age-Related Changes of Chondrogenic Growth Factors in Platelet-Rich Plasma. Operative Techniques in Orthopaedics, Volume 22, Issue 2, June 2012, Pages 49-55
    Species: Human
    Sample type: Plasma
  9. Lee YC., et al. BMP4 Promotes Prostate Tumor Growth in Bone through Osteogenesis. Cancer Research 71(15); 5194-203. DOI: 10.1158/0008-5472.CAN-10-4374
    Species: Human
    Sample type: Conditioned Media
  10. Mi, H. W., et al. "Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer." Gene therapy 18.5 (2011): 452-461.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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