Human BMP-6 ELISA

RayBio® Human BMP-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-BMP6-1
+

Antigen Information

Accession Number:
  • P22004
Gene ID:
  • 654
Gene Symbols:
  • BMP6
  • VGR
Protein Name & Synonyms:
Bone morphogenetic protein 6 (BMP-6) (VG-1-related protein) (VG-1-R) (VGR-1)
Target Species:
  • Human

Assay Format

Detection Range:
150 pg/ml - 50000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
150 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, BMP-4, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ogure-Nose S., Yoshino O., Akiyama I., et al. Serum concentration of bone morphogenetic proteins(BMPs) is not linked to serum anti-mullerian hormone(AMH) level. Integrative Molecular Medicine 2014, Volume 1(2): 26-28. doi: 10.15761/IMM.1000107
    Species: Human
    Sample type: Serum
  2. Zhang S., et al. Disordered hepcidin-ferroportin signaling promotes breast cancer growth. Cellular Signalling available online 3 August 2014. DOI: 10.1016/j.cellsig.2014.07.029
    Species: Human
    Sample type: Serum
  3. Seamon J, Wang X, Cui F, et al. Adenoviral Delivery of the VEGF and BMP-6 Genes to Rat Mesenchymal Stem Cells Potentiates Osteogenesis. Bone Marrow Res. 2013;2013:737580.
    Species: Human
    Sample type: Conditioned Media
  4. Overman, Janice R., et al. "Growth factor gene expression profiles of bone morphogenetic protein-2-treated human adipose stem cells seeded on calcium phosphate scaffolds in vitro." Biochimie 95.12 (2013): 2304-2313.
    Species: Human
    Sample type: Conditioned Media
  5. Lee YC., et al. BMP4 Promotes Prostate Tumor Growth in Bone through Osteogenesis. Cancer Research 71(15); 5194-203. DOI: 10.1158/0008-5472.CAN-10-4374
    Species: Human
    Sample type: Conditioned Media
  6. Vlacic-Zischke, Jelena, et al. "The influence of surface microroughness and hydrophilicity of titanium on the up-regulation of TGF?/BMP signalling in osteoblasts." Biomaterials 32.3 (2011): 665-671.
    Species: Human
    Sample type: Conditioned Media
  7. Wahlström, Ola, et al. "Acidic preparations of lysed platelets upregulate proliferative pathways in osteoblast-like cells as demonstrated by genome-wide microarray analysis." Platelets 22.6 (2011): 452-460.
    Species: Human
    Sample type: Cell Lysate
  8. Cui F., et al. VEGF and BMP-6 enhance bone formation mediated by cloned mouse osteoprogenitor cells. Growth Factors, October 2010; 28(5): 306-317. DOI: 10.3109/08977194.2010.484423
    Species: Human
    Sample type: Conditioned Media

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program