Human BMP-7 ELISA

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RayBio® Human BMP-7 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-BMP7-1
+

Antigen Information

Accession Number:
  • P18075
Gene ID:
  • 655
Gene Symbols:
  • BMP7
  • OP1
Protein Name & Synonyms:
Bone morphogenetic protein 7 (BMP-7) (Osteogenic protein 1) (OP-1) (Eptotermin alfa)
Target Species:
  • Human

Assay Format

Detection Range:
10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
10 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, BMP4, BMP-6, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GMCSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, MMP-2, MMP-3, MMP-9, MMP-10, MMP-13, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Demir N., Kolgelier S., Inkaya A., Sumer S., Demir L., Pehlivan S., Arslan M., Arpaci A. Are bone morphogenetic protein-7 (BMP-7) serum levels correlated with development of hepatic fibrosis? J Infect Dev Ctries 2014; 8(5):605-610.
    Species: Human
    Sample type: Serum
  2. Ogure-Nose S., Yoshino O., Akiyama I., et al. Serum concentration of bone morphogenetic proteins(BMPs) is not linked to serum anti-mullerian hormone(AMH) level. Integrative Molecular Medicine 2014, Volume 1(2): 26-28. doi: 10.15761/IMM.1000107
    Species: Human
    Sample type: Serum
  3. Odabas, S., et al. "Auricular cartilage repair using cryogel scaffolds loaded with BMP?7?expressing primary chondrocytes." Journal of tissue engineering and regenerative medicine 7.10 (2013): 831-840.
    Species: Rabbit
    Sample type: Conditioned Media
  4. Denizli, Naz?m, et al. "Bone morphogenetic protein-7 and disease progression in renal amyloidosis patients." Renal failure 35.8 (2013): 1112-1115.
    Species: Human
    Sample type: Serum
  5. A nanoparticulate injectable hydrogel as a tissue engineering scaffold for multiple growth factor delivery for bone regeneration
    Species: not specified
    Sample type: Nanomaterial
  6. Fiaschetti, G., et al. "Bone morphogenetic protein-7 is a MYC target with prosurvival functions in childhood medulloblastoma." Oncogene 30.25 (2011): 2823-2835.
    Species: Human
    Sample type: Conditioned Media
  7. Wahlström, Ola, et al. "Acidic preparations of lysed platelets upregulate proliferative pathways in osteoblast-like cells as demonstrated by genome-wide microarray analysis." Platelets 22.6 (2011): 452-460.
    Species: Human
    Sample type: Cell Lysate
  8. Mizuiri, Sonoo, et al. "Effluent markers related to epithelial mesenchymal transition with adjusted values for effluent cancer antigen 125 in peritoneal dialysis patients." International journal of nephrology 2011 (2011).
    Species: Human
    Sample type: Effluent
  9. Szeto C, Chow K, Kwan B, Lai K, Chung K, et al. The relationship between bone morphogenic protein-7 and peritoneal transport characteristics. Nephrol Dial Transplant. 2008; 23:2989-2994.
    Species: Human
    Sample type: Peritoneal Fluid
  10. El-Sabban ME, El-Khoury H, Hamdan-Khalil R, Sindet-Pedersen S, Bazarbachi A. Xenogenic bone matrix extracts induce osteblastic differentiation of human bone marrow-derived mesenchymal stem cells. Regen Med. 2007;2(4):383-390.
    Species: Equine
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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