25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

NexaTag^® Human Chordin-Like 2 qIPCR ELISA Kit

The bead-based NexaTag^® Human Chordin-Like 2 qIPCR ELISA Kit merges the specificity of sandwich-based ELISA, the sensitivity of real-time PCR, and the easy manipulation of microspheres to detect even the lowest levels of Chordin-Like 2 in serum, plasma, and cell culture supernatant samples.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	4-8 weeks
Species	Human
Specificity	The antibodies used in the kit are specific to its own protein.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Accession Number	Q6WN34
Gene Id	25884
Gene Symbols	CHRDL2, BNF1, CHL2, UNQ765/PRO1557
Protein Name / Synonyms	Chordin-like protein 2 (Breast tumor novel factor 1) (BNF-1) (Chordin-related protein 2)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	qPCR
Shipping Type	Blue ice
Storage	-20°C
Note	This NexaTag^® qIPCR ELISA utilizes an antibody pair and standard validated for colorimetric ELISA but not yet optimized for the qIPCR format. Therefore, a 4–8 week development phase is required following order placement. Upon completion, the kit will meet RayBiotech ELISA quality control standards, with finalized specifications (including sensitivity and detection range), QC data, and a completed manual available. Subsequent orders will have a typical 3–5 day lead time.

Product Features

Ultra-sensitivity: Up to 1,000x more sensitive than traditional ELISA
Ultra-low sample volume: Less than 10 µL needed
Specificity: Sandwich-based detection
Simplicity: Two steps processing
Efficiency: Get data within 4 hours
Multiplexing ready*

*Increase your throughput with RayBiotech's customizable multiplex bead-based immuno-PCR kits, designed for panels of up to 5 targets. This platform helps you save time, reduce costs, and decrease sample consumption by >2000% compared to traditional colorimetric ELISA. For more details, reach out to us at techsupport@raybiotech.com.

Kit Components

Component	Size / Qty
96-well V-bottom Plate	1 plate
96-well Plate Film	1 piece
96-well PCR Plate	1 plate
1X Assay Diluent	1 bottle
20X Wash Buffer Concentrate	1 bottle
Lyophilized Standard	1 vial
Antibody Coated Beads	1 vial
Oligonucleotide Conjugated Antibody	1 vial
1X Elution Buffer	1 vial
2X PCR Master Mix	1 vial
Primer Mix	1 vial
PCR Plate Film	1 piece

*The PCR plate used is a 0.2 mL, non-skirted 96-well plate (Thermo Fisher, cat. no. AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (techsupport@raybiotech.com).

Additional Materials Required

Real-time PCR instrument, Bio-Rad or QuantStudio™ recommended
Orbital 96-well plate shaker (with ability to reach 700 rpm)
RayBio^® 96-well Magnetic Plate Separator (Catalog #: BIQ-MPS) or similar
Optional: Automated microplate washer (BioTek 405 TS Washer recommended) with magnetic separation (RayBio^® 96-well Magnetic Plate Separator recommended)
Precision pipettes to deliver 1 μL to 1 mL volumes
Pipette tips (barrier tips are recommended to reduce contamination)
Adjustable 1-25 mL pipettes for reagent preparation
Centrifuge that can spin 96 well plates at 500 g
Nuclease-free water or deionized water
1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
Absorbent paper

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 5 μL of the bead and conjugate mix, and 5 μL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
Add 40 μL Assay Diluent and wash the plate using a magnetic plate separator or automated microplate washer.
Add 40 μL Elution buffer to each well. Incubate for 10 minutes at room temperature.
Prepare PCR/Primer Master Mix and pipette 15 μL into each well of the PCR plate.
Transfer 5 μL supernatant from V-bottom plate to the PCR plate.
Run real-time PCR.

Storage/Stability

Upon receiving the kit, store 2X PCR Master Mix, Primer Mix and Lyophilized Standard at -20°C. Store other components at 4°C. If stored at the proper temperature, the kit may be stored for up to 12 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (BIQ-PRELIM.pdf)

Product Features

Ultra-sensitivity: Up to 1,000x more sensitive than traditional ELISA
Ultra-low sample volume: Less than 10 µL needed
Specificity: Sandwich-based detection
Simplicity: Two steps processing
Efficiency: Get data within 4 hours
Multiplexing ready*

Kit Components

Component	Size / Qty
96-well V-bottom Plate	1 plate
96-well Plate Film	1 piece
96-well PCR Plate	1 plate
1X Assay Diluent	1 bottle
20X Wash Buffer Concentrate	1 bottle
Lyophilized Standard	1 vial
Antibody Coated Beads	1 vial
Oligonucleotide Conjugated Antibody	1 vial
1X Elution Buffer	1 vial
2X PCR Master Mix	1 vial
Primer Mix	1 vial
PCR Plate Film	1 piece

Additional Materials Required

Real-time PCR instrument, Bio-Rad or QuantStudio™ recommended
Orbital 96-well plate shaker (with ability to reach 700 rpm)
RayBio^® 96-well Magnetic Plate Separator (Catalog #: BIQ-MPS) or similar
Optional: Automated microplate washer (BioTek 405 TS Washer recommended) with magnetic separation (RayBio^® 96-well Magnetic Plate Separator recommended)
Precision pipettes to deliver 1 μL to 1 mL volumes
Pipette tips (barrier tips are recommended to reduce contamination)
Adjustable 1-25 mL pipettes for reagent preparation
Centrifuge that can spin 96 well plates at 500 g
Nuclease-free water or deionized water
1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
Absorbent paper

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 5 μL of the bead and conjugate mix, and 5 μL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
Add 40 μL Assay Diluent and wash the plate using a magnetic plate separator or automated microplate washer.
Add 40 μL Elution buffer to each well. Incubate for 10 minutes at room temperature.
Prepare PCR/Primer Master Mix and pipette 15 μL into each well of the PCR plate.
Transfer 5 μL supernatant from V-bottom plate to the PCR plate.
Run real-time PCR.

Featured Products

25% off all ELISA kits

25% off all ELISA kits

NexaTag^® Human Chordin-Like 2 qIPCR ELISA Kit