Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	49
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	ACE-2 Gene Symbol: ACE2 Gene ID: 59272 Accession #: Q9BYF1	Alpha-fetoprotein Gene Symbol: AFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiostatin Gene Symbol: PLG Gene ID: 5340 Accession #: P00747 ELISA Kit: RayBio Human Angiostatin ELISA
ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	Beta-2 Microglobulin Gene Symbol: B2M Gene ID: 567 Accession #: P61769 ELISA Kit: RayBio Human B2M ELISA	BCMA (TNFRSF17) Gene Symbol: TNFRSF17 Gene ID: 608 Accession #: Q02223 ELISA Kit: RayBio Human TNFRSF17 ELISA	beta IG-H3 Gene Symbol: TGFBI Gene ID: 7045 Accession #: Q15582 ELISA Kit: RayBio Human BIGH3 ELISA	CA125 Gene Symbol: MUC16 Gene ID: 94025 Accession #: Q8WXI7 ELISA Kit: RayBio Human CA-125 ELISA
CA15-3 Gene Symbol: MUC1 Gene ID: 4582 Accession #: P15941 ELISA Kit: RayBio Human CA15-3 ELISA	CA19-9 ELISA Kit: RayBio Human CA19-9 ELISA	CA9 Gene Symbol: CA9 Gene ID: 768 Accession #: Q16790 ELISA Kit: RayBio Human CA9 ELISA	Cathepsin S Gene Symbol: CTSS Gene ID: 1520 Accession #: P25774 ELISA Kit: RayBio Human Cathepsin S ELISA	HCC-1 (CCL14) Gene Symbol: CCL14 Gene ID: 6358 Accession #: Q16627 ELISA Kit: RayBio Human HCC-1 ELISA
6Ckine (CCL21) Gene Symbol: CCL21 Gene ID: 6366 Accession #: O00585 ELISA Kit: RayBio Human 6Ckine ELISA	CD23 Gene Symbol: FCER2 Gene ID: 2208 Accession #: P06734 ELISA Kit: RayBio Human CD23 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CEA ELISA Kit: RayBio Human CEA ELISA	CEACAM-1 Gene Symbol: CEACAM1 Gene ID: 634 Accession #: P13688 ELISA Kit: RayBio Human CEACAM-1 ELISA
Cripto-1 Gene Symbol: TDGF1 Gene ID: 6997 Accession #: P13385	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741 ELISA Kit: RayBio Human CRP ELISA	DAN Gene Symbol: PARN Gene ID: 5073 Accession #: O95453 ELISA Kit: RayBio Human DAN ELISA	Decorin Gene Symbol: DCN Gene ID: 1634 Accession #: P07585 ELISA Kit: RayBio Human Decorin ELISA	DKK-1 Gene Symbol: DKK1 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA
Dkk-3 Gene Symbol: DKK3 Gene ID: 27122 Accession #: Q9UBP4 ELISA Kit: RayBio Human Dkk-3 ELISA	Dkk-4 Gene Symbol: DKK4 Gene ID: 27121 Accession #: Q9UBT3 ELISA Kit: RayBio Human Dkk-4 ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Gene ID: 1803 Accession #: P27487 ELISA Kit: RayBio Human CD26 ELISA	E-Cadherin Gene Symbol: CDH1 Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA	EDA-A2 Gene Symbol: EDA Gene ID: 1896 Accession #: Q92838 ELISA Kit: RayBio Human EDA-A2 ELISA
EG-VEGF (PK1) Gene Symbol: PROK1 Gene ID: 84432 Accession #: P58294 ELISA Kit: RayBio Human EG-VEGF ELISA	TROP1 (EpCAM) Gene Symbol: EPCAM Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1 ELISA	ErbB2 Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626 ELISA Kit: RayBio Human ErbB2 ELISA	Erythropoietin R Gene Symbol: EPOR Gene ID: 2057 Accession #: P19235 ELISA Kit: RayBio Human EPOR ELISA	FSH Gene Symbol: CGA/FSHB Gene ID: 1081/2488 Accession #: P01215/P01225 ELISA Kit: RayBio Human FSH ELISA
HB-EGF Gene Symbol: HBEGF Gene ID: 1839 Accession #: Q99075 ELISA Kit: RayBio Human HB-EGF ELISA	hCG intact Gene Symbol: CGB Gene ID: 1082 Accession #: P01233 ELISA Kit: RayBio Human hCG ELISA	HVEM (TNFRSF14) Gene Symbol: TNFRSF14 Gene ID: 8764 Accession #: Q92956 ELISA Kit: RayBio Human HVEM ELISA	IL-13 R1	IL-17B Gene Symbol: IL17B Gene ID: 27190 Accession #: Q9UHF5 ELISA Kit: RayBio Human IL-17B ELISA
IL-17C Gene Symbol: IL17C Gene ID: 27189 Accession #: Q9P0M4	IL-17F Gene Symbol: IL17F Gene ID: 112744 Accession #: Q96PD4 ELISA Kit: RayBio Human IL-17F ELISA	IL-17 RA Gene Symbol: IL17RA Gene ID: 23765 Accession #: Q96F46 ELISA Kit: RayBio Human IL-17 R ELISA	Procalcitonin Gene Symbol: CALCA Gene ID: 796 Accession #: P01258 ELISA Kit: RayBio Human Procalcitonin ELISA	PSA-free Gene Symbol: KLK3 Gene ID: 354 Accession #: P07288 ELISA Kit: RayBio Human PSA-free ELISA
S100 B Gene Symbol: S100B Gene ID: 6285 Accession #: P04271	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Gene ID: 6469 Accession #: Q15465 ELISA Kit: RayBio Human ShhN ELISA	Thyroglobulin Gene Symbol: TG Gene ID: 7038 Accession #: P01266 ELISA Kit: RayBio Human Thyroglobulin ELISA	Ubiquitin+1 Gene Symbol: RPS27A Gene ID: 6233 Accession #: P62979 ELISA Kit: RayBio Human Ubiquitin+1 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C10 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	ACE-2 Gene Symbol: ACE2 Gene ID: 59272 Accession #: Q9BYF1	Alpha-fetoprotein Gene Symbol: AFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiostatin Gene Symbol: PLG Gene ID: 5340 Accession #: P00747 ELISA Kit: RayBio Human Angiostatin ELISA
ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	Beta-2 Microglobulin Gene Symbol: B2M Gene ID: 567 Accession #: P61769 ELISA Kit: RayBio Human B2M ELISA	BCMA (TNFRSF17) Gene Symbol: TNFRSF17 Gene ID: 608 Accession #: Q02223 ELISA Kit: RayBio Human TNFRSF17 ELISA	beta IG-H3 Gene Symbol: TGFBI Gene ID: 7045 Accession #: Q15582 ELISA Kit: RayBio Human BIGH3 ELISA	CA125 Gene Symbol: MUC16 Gene ID: 94025 Accession #: Q8WXI7 ELISA Kit: RayBio Human CA-125 ELISA
CA15-3 Gene Symbol: MUC1 Gene ID: 4582 Accession #: P15941 ELISA Kit: RayBio Human CA15-3 ELISA	CA19-9 ELISA Kit: RayBio Human CA19-9 ELISA	CA9 Gene Symbol: CA9 Gene ID: 768 Accession #: Q16790 ELISA Kit: RayBio Human CA9 ELISA	Cathepsin S Gene Symbol: CTSS Gene ID: 1520 Accession #: P25774 ELISA Kit: RayBio Human Cathepsin S ELISA	HCC-1 (CCL14) Gene Symbol: CCL14 Gene ID: 6358 Accession #: Q16627 ELISA Kit: RayBio Human HCC-1 ELISA
6Ckine (CCL21) Gene Symbol: CCL21 Gene ID: 6366 Accession #: O00585 ELISA Kit: RayBio Human 6Ckine ELISA	CD23 Gene Symbol: FCER2 Gene ID: 2208 Accession #: P06734 ELISA Kit: RayBio Human CD23 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CEA ELISA Kit: RayBio Human CEA ELISA	CEACAM-1 Gene Symbol: CEACAM1 Gene ID: 634 Accession #: P13688 ELISA Kit: RayBio Human CEACAM-1 ELISA
Cripto-1 Gene Symbol: TDGF1 Gene ID: 6997 Accession #: P13385	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741 ELISA Kit: RayBio Human CRP ELISA	DAN Gene Symbol: PARN Gene ID: 5073 Accession #: O95453 ELISA Kit: RayBio Human DAN ELISA	Decorin Gene Symbol: DCN Gene ID: 1634 Accession #: P07585 ELISA Kit: RayBio Human Decorin ELISA	DKK-1 Gene Symbol: DKK1 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA
Dkk-3 Gene Symbol: DKK3 Gene ID: 27122 Accession #: Q9UBP4 ELISA Kit: RayBio Human Dkk-3 ELISA	Dkk-4 Gene Symbol: DKK4 Gene ID: 27121 Accession #: Q9UBT3 ELISA Kit: RayBio Human Dkk-4 ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Gene ID: 1803 Accession #: P27487 ELISA Kit: RayBio Human CD26 ELISA	E-Cadherin Gene Symbol: CDH1 Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA	EDA-A2 Gene Symbol: EDA Gene ID: 1896 Accession #: Q92838 ELISA Kit: RayBio Human EDA-A2 ELISA
EG-VEGF (PK1) Gene Symbol: PROK1 Gene ID: 84432 Accession #: P58294 ELISA Kit: RayBio Human EG-VEGF ELISA	TROP1 (EpCAM) Gene Symbol: EPCAM Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1 ELISA	ErbB2 Gene Symbol: ErbB2 Gene ID: 2064 Accession #: P04626 ELISA Kit: RayBio Human ErbB2 ELISA	Erythropoietin R Gene Symbol: EPOR Gene ID: 2057 Accession #: P19235 ELISA Kit: RayBio Human EPOR ELISA	FSH Gene Symbol: CGA/FSHB Gene ID: 1081/2488 Accession #: P01215/P01225 ELISA Kit: RayBio Human FSH ELISA
HB-EGF Gene Symbol: HBEGF Gene ID: 1839 Accession #: Q99075 ELISA Kit: RayBio Human HB-EGF ELISA	hCG intact Gene Symbol: CGB Gene ID: 1082 Accession #: P01233 ELISA Kit: RayBio Human hCG ELISA	HVEM (TNFRSF14) Gene Symbol: TNFRSF14 Gene ID: 8764 Accession #: Q92956 ELISA Kit: RayBio Human HVEM ELISA	IL-13 R1	IL-17B Gene Symbol: IL17B Gene ID: 27190 Accession #: Q9UHF5 ELISA Kit: RayBio Human IL-17B ELISA
IL-17C Gene Symbol: IL17C Gene ID: 27189 Accession #: Q9P0M4	IL-17F Gene Symbol: IL17F Gene ID: 112744 Accession #: Q96PD4 ELISA Kit: RayBio Human IL-17F ELISA	IL-17 RA Gene Symbol: IL17RA Gene ID: 23765 Accession #: Q96F46 ELISA Kit: RayBio Human IL-17 R ELISA	Procalcitonin Gene Symbol: CALCA Gene ID: 796 Accession #: P01258 ELISA Kit: RayBio Human Procalcitonin ELISA	PSA-free Gene Symbol: KLK3 Gene ID: 354 Accession #: P07288 ELISA Kit: RayBio Human PSA-free ELISA
S100 B Gene Symbol: S100B Gene ID: 6285 Accession #: P04271	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Gene ID: 6469 Accession #: Q15465 ELISA Kit: RayBio Human ShhN ELISA	Thyroglobulin Gene Symbol: TG Gene ID: 7038 Accession #: P01266 ELISA Kit: RayBio Human Thyroglobulin ELISA	Ubiquitin+1 Gene Symbol: RPS27A Gene ID: 6233 Accession #: P62979 ELISA Kit: RayBio Human Ubiquitin+1 ELISA

Application Notes

Suggested Application

Kit Components

Human Cytokine Antibody Array C10 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Featured Products

Human Cytokine Array C10

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

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Human Cytokine Array C10

Human Cytokine Array C10

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Have this product in hand?

Human Cytokine Array C10

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Featured Products

Human Cytokine Array C10

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

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Human Cytokine Array C10

Human Cytokine Array C10

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Human Cytokine Array C10

Featured Products