QAH-CAA-440 was used to profile serum cytokines in Guillain-Barré syndrome patients and healthy controls, with representative antibody array panels highlighting differential expression of multiple immune-related proteins between disease and control samples. Figure adapted from Li et al., Scientific Reports, 2023. QAH-CAA-440 was used for high-content serum cytokine profiling to identify biomarkers associated with the prethrombotic state in recurrent spontaneous abortion, with the image showing a representative portion of the full 440-target antibody array. Figure adapted from Wu et al., J Cell Mol Med, 2021. QAH-CAA-440 was used for high-content plasma cytokine profiling in neuromyelitis optica spectrum disorder patients and healthy controls, identifying differentially expressed immune-related factors associated with disease status. Figure adapted from Xie et al., Journal of Translational Medicine, 2024. QAH-CAA-440 was used to profile serum cytokines in patients with deep vein thrombosis and non-deep vein thrombosis, with heat map analysis revealing distinct cytokine expression patterns associated with thrombus formation. Figure adapted from Wang et al., BMC Cardiovascular Disorders, 2024. QAH-CAA-440 was used to profile plasma proteins in multiple sclerosis patients and healthy controls, with heat map analysis revealing distinct disease-associated protein expression patterns. Figure adapted from Zhou et al., Frontiers in Pharmacology, 2024.
Kit Components
Human Cytokine Array Q440 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual
Additional Materials Required
Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
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Assay Procedure Summary
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.