Human E-Selectin ELISA

$398.00
ELH-Eselectin-1

RayBio® Human E-Selectin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
30 pg/ml

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Detection Range
30 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma)
50 - 200 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Species Detected
Human
Protein Name & Synonyms
E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)
Accession Number
Gene Names
SELE, ELAM1
Gene ID
6401

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • SELE

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
    Species: Human
    Sample Type: Plasma

  2. Siristatidis C., Askoxylaki M., Varounis C., et al. E-selectin, resistin and reactive oxygen species levels in GnRH -agonist and -antagonist protocols in IVF/ICSI: a prospective cohort study. J Assist Reprod Genet. 2015 Jun;32(6):959-67. doi: 10.1007/s10815-015-0489-z.
    Species: Human
    Sample Type: Serum

  3. Kesebir S, Turan Ç, Süner Ö, Yaylacı ET. Increased ICAM, VCAM, and E-selectin levels in first manic episode. Bipolar Disord 2014: Ahead of Print Oct 2014 DOI:10.1111/bdi.12269
    Species: Human
    Sample Type: Plasma

  4. Farjo KM, Farjo RA, Halsey S, Moiseyev G, Ma J. Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism. Molecular and Cellular Biology 2012;32(24):5103-5115. doi:10.1128/MCB.00820-12.
    Species: Human
    Sample Type: Conditioned Media

  5. Sadik N., Mohamed W., Ahmed M. The association of receptor of advanced glycated end products and inflammatory mediators contributes to endothelial dysfunction in a prospective study of acute kidney injury patients with sepsis. Mol Cell Biochem. 2012 Jan;359(1-2):73-81. doi: 10.1007/s11010-011-1001-4.
    Species: Human
    Sample Type: Serum

  6. Daoud E., Abdelaziz A., Hassan N. Isolated coronary artery ectasia debate: Inflammation versus atherosclerosis The Egyptian Heart Journal Volume 64, Issue 4, December 2012, Pages 185-190
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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