Human EGF ELISA

RayBio® Human EGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-EGF-1
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Antigen Information

Accession Number:
  • P01133
Gene ID:
  • 1950
Gene Symbols:
  • EGF
Protein Name & Synonyms:
Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Lim Y., Yoo J., Kim M., et al. GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. Mol Cancer Ther. 2016 Feb;15(2):251-63. doi: 10.1158/1535-7163.MCT-15-0679
    Species: Human
    Sample type: Conditioned Media
  2. El-Bendary M., Neamatallah M., El-Maksoud M., et al. EPIDERMAL GROWTH FACTOR GENETIC POLYMORPHISM AND ITS CIRCULATING SERUM LEVEL PREDICT THE RISK OF HEPATOCELLULAR CARCINOMA IN EGYPTIAN PATIENTS WITH HCV (Genotype-4)-RELATED CIRRHOSIS.
    Species: Human
    Sample type: Plasma
  3. Lucas L., Tellez C., Castilho M., et al. Development of a sensitive, stable and EGFRspecific molecular imaging agent for surface enhanced Raman spectroscopy. J. Raman Spectrosc. 2015, 46, 434-446. DOI 10.1002/jrs.4678
    Species: Human
    Sample type: Nanomaterial
  4. Plagg B., Marksteiner J., Kniewallner K., et al. Platelet dysfunction in hypercholesterolemia mice, two Alzheimer's disease mouse models and in human patients with Alzheimer's disease. Biogerontology. 2015 Aug;16(4):543-58. doi: 10.1007/s10522-015-9580-1.
    Species: Human
    Sample type: Platelet Releasate
  5. Zhang Y., Lin P., Jiang H., et al. Extensive serum biomarker analysis in patients with ST segment elevation myocardial infarction (STEMI). Cytokine. 2015 Dec;76(2):356-62. doi: 10.1016/j.cyto.2015.06.015.
    Species: Human
    Sample type: Serum
  6. Al-Shammari A. Allak W., Umran M., et al. Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured in Vitro. Advances in Breast Cancer Research, 2015, 4, 100-108, http://dx.doi.org/10.4236/abcr.2015.44011
    Species: Human
    Sample type: Conditioned Media
  7. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  8. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample type: Tissue Lysate
  9. Koob TJ., Lim JJ., Massee M., Zabek N., Denoziere G. Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation. J Biomed Mater Res B Appl Biomater. 2014 Mar 25 [Epub ahead of print]
    Species: Human
    Sample type: Tissue Lysate
  10. Miyabayashi K, Ijichi H, Mohri D, et al. Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals. Cancer Res. 2013 Apr 1;73(7):2221-2234.
    Species: Human
    Sample type: Cell Lysate
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.