Human Endoglin ELISA

RayBio® Human Endoglin (CD105) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELH-Endoglin-1
+

Assay Format

Detection Range:
10 pg/ml - 3000 pg/ml
Recommended Dilution (serum & plasma):
20 - 200 fold
Sensitivity:
10 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P17813
Gene ID:
  • 2022
Gene Symbols:
  • END
  • ENG
Protein Name & Synonyms:
Endoglin (CD antigen CD105)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ambarwati, Dewi, et al. "Punica granatum fruit extract inhibits the production of pro-inflammatory cytokines and angiogenic factors of HUVEC cells induced by plasma from patients with pre-eclampsia." Clinical Nutrition Experimental 15 (2017): 9-14.
    Species: Human
    Sample type: Conditioned Media (HUVEC medium )
  2. Zhang Y., Yang H., Long Y., Li W. Circular RNA in blood corpuscles combined with plasma protein factor for early prediction of pre-eclampsia. BJOG. 2016 Feb 5. doi: 10.1111/1471-0528.13897.
    Species: Human
    Sample type: Plasma (Normal and Preeclampsia patients)
  3. Bassyouni I., El-Shazly R., Azkalany G., et al. Clinical significance of soluble-endoglin levels in systemic lupus erythematosus: possible association with anti-phospholipid syndrome. Lupus. 2012 Dec;21(14):1565-70. doi: 10.1177/0961203312460115.
    Species: Human
    Sample type: Plasma (Lupus patients with anti-phospholipid syndrome)
  4. Küçükçongar, A., Yenicesu, ?., Tümer, L., Kasapkara, Ç. S., Ezgü, F. S., Pa?ao?lu, Ö., ... & Hasano?lu, A. (2013). Apheresis-inducible cytokine pattern change in children with homozygous familial hypercholesterolemia. Transfusion and Apheresis Science, 48(3), 391-396.
    Species: Human
    Sample type: Plasma (children with homozygous familial hypercholesterolemia)
  5. Ambarwati, Dewi, et al. "Punica granatum fruit extract inhibits the production of pro-inflammatory cytokines and angiogenic factors of HUVEC cells induced by plasma from patients with pre-eclampsia." Clinical Nutrition Experimental 15 (2017): 9-14.
    Species: Human
    Sample type: Conditioned Media (HUVEC medium )

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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