Human G-CSF ELISA

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RayBio® Human GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-GCSF-1
+

Antigen Information

Accession Number:
  • P09919
Gene ID:
  • 1440
Gene Symbols:
  • C17ORF33
  • CSF3
  • GCSF
Protein Name & Synonyms:
Granulocyte colony-stimulating factor (G-CSF) (Pluripoietin) (Filgrastim) (Lenograstim)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 500 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wu Y., Liu H., Huang T., et al. A phase II double-blinded study to evaluate the efficacy of EW02 in reducing chemotherapy-induced neutropenia in breast cancer. Oncol Lett. 2015 Sep;10(3):1793-1798.
    Species: Human
    Sample type: Serum
  2. Batista R., et al. Phenotypic features of first-generation transgenic goats for human granulocyte-colony stimulation factor production in milk. Biotechnology Letters Online First June 2014. DOI 10.1007/s10529-014-1588-0
    Species: Human
    Sample type: Serum
  3. El-Ganzoury M., et al. Enteral Granulocyte-Colony Stimulating Factor and Erythropoietin Early in Life Improves Feeding Tolerance in Preterm Infants: A Randomized Controlled Trial. The Journal of Pediatrics, Aug 2014, epub ahead of print. DOI: 10.1016/j.jpeds.2014.07.034
    Species: Human
    Sample type: Serum
  4. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  5. Koob TJ., Lim JJ., Massee M., Zabek N., Denoziere G. Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation. J Biomed Mater Res B Appl Biomater. 2014 Mar 25 [Epub ahead of print]
    Species: Human
    Sample type: Tissue Lysate
  6. Lee, YH., Shynlova, O., et al. Stretch-induced human myometrial cytokines enhance immune cell recruitment via endothelial activation. Cellular & Molecular Immunology 2014. Epub Ahead of Print DOI: doi: 10.1038/cmi.2014.39
    Species: Human
    Sample type: Conditioned Media
  7. Nair N., Chidambareswaren M., Manjula S. Enhanced Heterologous Expression of Biologically Active Human Granulocyte Colony Stimulating Factor in Transgenic Tobacco BY-2 Cells by Localization to Endoplasmic Reticulum. Molecular Biotechnology, 2014 Epub Ahead of Print. DOI 10.1007/s12033-014-9765-x
    Species: Human
    Sample type: Conditioned Media
  8. Moura RR, Albuquerque ES, Henrique C, et al. Dynamics of Recombinant hG-CSF in Transgenic Goat: Preliminary Study in the Founder during Hormonally Induced Lactation. Anim Biotechnol. 2013;274(1):10-14.
    Species: Human
    Sample type: Serum
  9. Schepers, Koen, et al. "Myeloproliferative neoplasia remodels the endosteal bone marrow niche into a self-reinforcing leukemic niche." Cell stem cell 13.3 (2013): 285-299.
    Species: Human
    Sample type: Conditioned Media
  10. Loving, Crystal L., et al. "Porcine granulocyte-colony stimulating factor (G-CSF) delivered via replication-defective adenovirus induces a sustained increase in circulating peripheral blood neutrophils." Biologicals 41.6 (2013): 368-376.
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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