Human GRO-alpha ELISA

$398.00
ELH-GROa-1

RayBio® Human GRO alpha (CXCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
2 pg/ml

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Detection Range
2 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Growth-regulated alpha protein (C-X-C motif chemokine 1) (GRO-alpha(1-73)) (Melanoma growth stimulatory activity) (MGSA) (Neutrophil-activating protein 3) (NAP-3)
Accession Number
Gene Names
CXCL1, GRO, GRO1, GROA, MGSA, SCYB1
Gene ID
2919

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CXCL1

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Garcia J., Carvajal-Gil J., Guerrero-Bonmatty R. Altered release of chemokines by phagocytes from fibromyalgia patients: a pilot study. Innate Immun. 2016 Jan;22(1):3-8. doi: 10.1177/1753425915602959.
    Species: Human
    Sample Type: Conditioned Media

  2. Duckworth C., Zhang L., Carroll S., et al. Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression. Oncogene. 2016 Aug 4;35(31):4036-47. doi: 10.1038/onc.2015.472.

  3. Zhou X., Gu J., Gu Y., et al. Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation Pattern Induction. Cell Physiol Biochem. 2015;35(6):2383-401. doi: 10.1159/000374040
    Species: Human
    Sample Type: Conditioned Media

  4. Spaks A., Jaunalksne I., Spaka I., et al. Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer. Anticancer Res. 2015 Dec;35(12):6979-83.

  5. Lv M., et al. miR141-CXCL1-CXCR2 Signaling-Induced Treg Recruitment Regulates Metastases and Survival of Non-Small Cell Lung Cancer. Molecular Cancer Therapeutics. OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448
    Species: Human
    Sample Type: Conditioned Media

  6. Rajajendram R., Tham CL., Akhtar MN., et al. Inhibition of Epithelial CC-Family Chemokine Synthesis by the Synthetic Chalcone DMPF-1 via Disruption of NF-κ Nuclear Translocation and Suppression of Experimental Asthma in Mice
    Species: Human
    Sample Type: Conditioned Media

  7. Fujiwara S., Nagai H., Oniki S., et al. Interleukin (IL)-17 versus IL-27: opposite effects on tumor necrosis factor-α-mediated chemokine production in human keratinocytes. Exp Dermatol. 2012 Jan;21(1):70-2. doi: 10.1111/j.1600-0625.2011.01384.x
    Species: Human
    Sample Type: Conditioned Media

  8. LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ. Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer. Molecular Cancer Therapeutics 2012;11(7):1389-1399. doi:10.1158/1535-7163.MCT-11-0887.
    Species: Human
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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