Human HGF ELISA

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ELH-HGF-1

RayBio® Human HGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Antigen Information

Accession Number:
  • P14210
Gene ID:
  • 3082
Gene Symbol:
  • HGF
  • HPTA
Protein Name & Synonyms:
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain]
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Drebert Z., Bracke M., Beck I. Glucocorticoids and the non-steroidal selective glucocorticoid receptor modulator, compound A, differentially affect colon cancer-derived myofibroblasts. J Steroid Biochem Mol Biol. 2015 May;149:92-105. doi: 10.1016/j.jsbmb.2015.02.002.
    Species: Human
    Sample type: Conditioned Media
  2. Nowak M., Sieminska L., Karpe J., et al. Serum concentrations of HGF and IL-8 in patients with active Graves' orbitopathy before and after methylprednisolone therapy. J Endocrinol Invest. 2016 Jan;39(1):63-72. doi: 10.1007/s40618-015-0322-7.
    Species: Human
    Sample type: Serum
  3. Fernandez-Yunquera A., et al. Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients. World J Transplant 2014; 4(2): 133-140. http://dx.doi.org/10.5500/wjt.v4.i2.133
    Species: Human
    Sample type: Serum
  4. Jung W., Han S., Kim S., Kim M., Lee J., Seo K., Youn H., Lee H. Stimulatory Effect of HGF-Overexpressing Adipose Tissue-Derived Mesenchymal Stem Cells on Thymus Regeneration in a Rat Thymus Involution Model. Cell Biology International Accepted May 7 2014,
    Species: Human
    Sample type: Conditioned Media
  5. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  6. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample type: Tissue Lysate
  7. Stewart MD., Ramani VC., Sanderson RD. Shed Syndecan-1 Translocates to the Nucleus of Cells Delivering Growth Factors and Inhibiting Histone Acetylation: A NOVEL MECHANISM OF TUMOR-HOST CROSS-TALK. mJ Biol Chem. 2015 Jan 9;290(2):941-9. doi: 10.1074/jbc.M114.608455.
    Species: Human
    Sample type: Conditioned Media
  8. Khan R, Sharma M, Kumar L, et al. Interrelationship and expression profiling of cyclooxygenase and angiogenic factors in Indian patients with multiple myeloma. Ann Hematol. 2013 Jan;92(1):101-109.
    Species: Human
    Sample type: Serum
  9. Lee EJ, Park H-W, Jeon H-J, et al. Potentiated therapeutic angiogenesis by primed human mesenchymal stem cells in a mouse model of hindlimb ischemia. Regen Med. 2013 May;8(3):283-293.
    Species: Human
    Sample type: Conditioned Media
  10. ?ahin, Nur, et al. "The levels of hepatocyte growth factor in serum and follicular fluid and the expression of c-Met in granulosa cells in patients with polycystic ovary syndrome." Fertility and sterility 99.1 (2013): 264-269.
    Species: Human
    Sample type: Follicular Fluid

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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