RayBio® Human HGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

3 pg/ml

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Detection Range
3 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data


This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Protein Name & Synonyms
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF)
Accession Number
Gene Names
Gene ID

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • HGF

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Method of Detection:
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Drebert Z., Bracke M., Beck I. Glucocorticoids and the non-steroidal selective glucocorticoid receptor modulator, compound A, differentially affect colon cancer-derived myofibroblasts. J Steroid Biochem Mol Biol. 2015 May;149:92-105. doi: 10.1016/j.jsbmb.2015.02.002.
    Species: Human
    Sample Type: Conditioned Media

  2. Nowak M., Sieminska L., Karpe J., et al. Serum concentrations of HGF and IL-8 in patients with active Graves' orbitopathy before and after methylprednisolone therapy. J Endocrinol Invest. 2016 Jan;39(1):63-72. doi: 10.1007/s40618-015-0322-7.
    Species: Human
    Sample Type: Serum

  3. Fernandez-Yunquera A., et al. Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients. World J Transplant 2014; 4(2): 133-140.
    Species: Human
    Sample Type: Serum

  4. Jung W., Han S., Kim S., Kim M., Lee J., Seo K., Youn H., Lee H. Stimulatory Effect of HGF-Overexpressing Adipose Tissue-Derived Mesenchymal Stem Cells on Thymus Regeneration in a Rat Thymus Involution Model. Cell Biology International Accepted May 7 2014,
    Species: Human
    Sample Type: Conditioned Media

  5. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample Type: Tissue Lysate

  6. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample Type: Tissue Lysate

  7. Stewart MD., Ramani VC., Sanderson RD. Shed Syndecan-1 Translocates to the Nucleus of Cells Delivering Growth Factors and Inhibiting Histone Acetylation: A NOVEL MECHANISM OF TUMOR-HOST CROSS-TALK. mJ Biol Chem. 2015 Jan 9;290(2):941-9. doi: 10.1074/jbc.M114.608455.
    Species: Human
    Sample Type: Conditioned Media

  8. Khan R, Sharma M, Kumar L, et al. Interrelationship and expression profiling of cyclooxygenase and angiogenic factors in Indian patients with multiple myeloma. Ann Hematol. 2013 Jan;92(1):101-109.
    Species: Human
    Sample Type: Serum

  9. Lee EJ, Park H-W, Jeon H-J, et al. Potentiated therapeutic angiogenesis by primed human mesenchymal stem cells in a mouse model of hindlimb ischemia. Regen Med. 2013 May;8(3):283-293.
    Species: Human
    Sample Type: Conditioned Media

  10. Najar M., Raicevic G., Fayyad-Kazan H., et al. Immune-related antigens, surface molecules and regulatory factors in human-derived mesenchymal stromal cells: the expression and impact of inflammatory priming. Stem Cell Rev. 2012 Dec;8(4):1188-98. doi: 10.1007/s12015-012-9408-1.
    Species: Human
    Sample Type: Conditioned Media

  11. Nazarov I, Lee JW, Soupene E, et al. Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. Stem Cells Translational Medicine. 2012;1(5):359-372. doi:10.5966/sctm.2011-0021.
    Species: Human
    Sample Type: Conditioned Media

  12. Dua R., et al. Detection of Hepatocyte Growth Factor (HGF) Ligand-c-MET Receptor Activation in Formalin-Fixed Paraffin Embedded Specimens by a Novel Proximity Assay. Published: January 21, 2011 DOI: 10.1371/journal.pone.0015932
    Species: Human
    Sample Type: Cell Lysate

  13. Tyan, Shiaw-Wei, et al. "Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis." PloS one 6.1 (2011): e15313.

  14. Ali Jalili, Neeta Shirvaikar, Leah A. Marquez-Curtis, A. Robert Turner, and Anna Janowska-Wieczorek. Stem Cells and Development. August 2010, 19(8): 1143-1151. doi:10.1089/scd.2009.0376.
    Species: Human
    Sample Type: Plasma

  15. The HGF/c-Met Axis Synergizes with G-CSF in the Mobilization of Hematopoietic Stem/Progenitor Cells
    Species: Human
    Sample Type: Conditioned Media

  16. Hsieh CS., et al. Evaluation of hepatocyte growth factor in patients with biliary atresia. Journal of Pediatric Surgery Volume 43, Issue 7, July 2008, Pages 1333-1337
    Species: Human
    Sample Type: Plasma

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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