We used this kit to check the IFN-gamma levels in human melanoma cell lines. It is very sensitive and the instructions are easy to follow. But it took 5-6 hours for the whole process. We are very satisfied with this product.

Review by Teresa, from Thomas Jefferson University, on Apr 25, 2024

This kit is easy to use and the instructions are straightforward. It takes about 5 hours for the whole protocol. The preparation of the reagents takes some time. But you can start with the first incubation which is 2.5 hours first, then prepare the rest of the reagents during that incubation time. The result is pretty good with the standard compound provided in the kit to make a standard curve. It is on 30% OFF sale now. I just bought another two-plate kit. Hurry! :)

Review by TZ, from Thomas Jefferson University, on Apr 18, 2024

Well trusted material for my analysis

Review by Carlos A. Barreto Gamarra, from University of Puerto Rico, on Sep 1, 2022

we used this kit to detect IFN gamma in iPSC-derived CNS cells. it works fine. it took about 4.5 hours to complete the assay which is reasonable.

Review by Ming, from Mayo Clinic, on May 11, 2022

Plasma & Urine were run at 2-fold dilution. Urine samples were BLQ. Plasma samples had very low concentration. Our samples and too low of a concentration to be measured by this assay.

Review by Anonymous, from University of Michigan, on Mar 14, 2022

This ELISA kit was used for quantification of IFN-gamma in the supernatants of SEB-stimulated peripheral blood mononuclear cells in combination with conventional chemotherapeutics. The variability of the technical replicates was low and the data obtained very repetitive.

Review by MARINA K, from KYNAN Pharma, on Jul 17, 2021

This human IFNg ELISA kit works very well. Steps included in the manufacturer's protocol were easy to follow and all the necessary materials were provided. The snap wells come in handy when running multiple experiments in small batches for optimization of the sample. We ran human plasma samples and the standards ran evenly and the detection concentration fell within the provided range which is rare for quantifying IFNg levels in human samples.

Review by Shreya Deb, from University of Illinois at Chicago, on Oct 31, 2020

This is the first time I've run an Interferon-g ELISA and I'm very happy with the results. I typically use the ELISpot platform and I'm happy that the results with this kit are far superior- very little background and fairly sensitive with the detection. I may try the newer version (with the qRT-PCR) readout for subsequent experiments to compare between kits.

Review by Aish S, from Mayo Clinic, on Jan 1, 2020

This human IFNg ELISA kit is good. Steps were clear and all necessary materials were provided. Since we ran multiple smaller experiments, snap wells were really handy. We used BAL from mice lungs and though the standard ran evenly, samples showed low IFNg levels.

Review by Hesper Wong, from University of Pittsburgh, on Jul 23, 2019

The human IFNg ELISA kit is easy to follow and reproducible. We used the kit with IFNg-secreting human cell lines following the manufacturer's protocol after the cells were treated with LPS. The supernatant was used to detect the IFNg. The kit worked perfectly when detecting high content of IFNg. However, when the IFNg content is low in the samples, more repeats may be needed as the sensitivity or accuracy of the kit is becoming low, too. Overall, the kit works well in detecting IFNg.

Review by Rosa Ignacio, on Jan 24, 2018