Human IGF-1 ELISA

RayBio® Human IGF-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-IGF1-1
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Antigen Information

Accession Number:
  • P05019
Gene ID:
  • 3479
Gene Symbols:
  • IBP1
  • IGF1
Protein Name & Synonyms:
Insulin-like growth factor I (IGF-I) (Mechano growth factor) (MGF) (Somatomedin-C)
Target Species:
  • Human

Assay Format

Detection Range:
0.1 ng/ml - 30 ng/ml
Recommended Dilution (serum & plasma):
2 - 20 fold
Sensitivity:
100 pg/ml

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Specificity:
This antibody pair can only detect a free form of human IGF-1. It does not detect a complex, such as binding with IGFBPs. This IGF-1 ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Meeks S., Van Haitsma K., Mast B., et al. Psychological and social resources relate to biomarkers of allostasis in newly admitted nursing home residents. Aging Ment Health. 2016;20(1):88-99. doi: 10.1080/13607863.2015.1072796
    Species: Human
    Sample type: Plasma
  2. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample type: Conditioned Media
  3. Shen C., Lie P., Miao T., et al. Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis. Mol Med Rep. 2015 Jul;12(1):20-30. doi: 10.3892/mmr.2015.3409
    Species: Human
    Sample type: Conditioned Media
  4. Pickard A, McDade SS, McFarland M, McCluggage WG, Wheeler CM, McCance DJ (2015) HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog 11(6): e1004988. doi:10.1371/journal.ppat.1004988
    Species: Human
    Sample type: Conditioned Media
  5. Krogh J., Rostrup E., Thomsen C., Elfving B., Videbech P., Nordentoft M. The effect of exercise on hippocampal volume and neurotrophines in patients with Major Depression - a randomized clinical trial. Journal of Affective Disorders Available online 23 April 2014 [Epub Ahead of Print]
    Species: Human
    Sample type: Serum
  6. Ritsche K., et al. Exercise-Induced growth hormone during acute sleep deprivation. Physiol Rep, 2(10), 2014, e12166, doi: 10.14814/phy2.12166
    Species: Human
    Sample type: Serum
  7. Stoppoloni D., et al. Effect of Glucosamine and its peptidyl-derivative on the production of extracellular matrix components by human primary chondrocytes. Osteoarthritis and Cartilage Sept 2014, epub ahead of print. http://dx.doi.org/10.1016/j.joca.2014.09.005
    Species: Human
    Sample type: Conditioned Media
  8. Aucouturier J, Thivel D, Isacco L, et al. Combined food intake and exercise unmask different hormonal responses in lean and obese children. Appl Physiol Nutr Metab. 2013;38(6): 638-643
    Species: Human
    Sample type: Plasma
  9. Khalil RM, El-Bahrawy HA, El-Ashmawy NE, Darwish H. L-Carnitine Decreases Her2/neu in Breast Cancer Patients Treated with Tamoxifen. IOSR J Pharm Biol Sci. 2013;5(2):91-98.
    Species: Human
    Sample type: Serum
  10. De Barros, Sandra, et al. "Aging-related decrease of human ASC angiogenic potential is reversed by hypoxia preconditioning through ROS production." Molecular Therapy 21.2 (2013): 399-408.
    Species: Human
    Sample type: Conditioned Media
The kit works well and is reactive with His-tagged human IGF1, as well. Results are reproducible and the whole experiment can be performed in ~5 hours.

University of Michigan
The human IGF-1 ELISA kit (ELH-IGF1-1) is easy to work with and reproducible. Overall we are very happy with these kits.

Lipscomb Univeristy
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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