Human IL-1 alpha ELISA

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ELH-IL1a-1

RayBio® Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Antigen Information

Accession Number:
  • P01583
Gene ID:
  • 3552
Gene Symbols:
  • IL1A
  • IL1F1
Protein Name & Synonyms:
Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
Target Species:
  • Human

Assay Format

Detection Range:
0.5 pg/ml - 300 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
0.5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Odewumi C., Latinwo L., Sinclair A., et al. Effect of cadmium on the expression levels of interleukin-1? and interleukin-10 cytokines in human lung cells. Mol Med Rep. 2015 Nov;12(5):6422-6. doi: 10.3892/mmr.2015.4316.
    Species: Human
    Sample type: Conditioned Media
  2. Abed chabuk S., Al-Momen A., Alsamaraee I. Correlation Between Interleukins 1 ?,6 and Echocardiographic Results in Patients with Heart Failure. Journal of Babylon University/Pure and Applied Sciences/ No.(4)/ Vol.(22): 2014
    Species: Human
    Sample type: Serum
  3. Wu SJ, Lu TM, Lai MN, Ng LT. Immunomodulatory Activities of Medicinal Mushroom Grifola frondosa Extract and Its Bioactive Constituent. Am J Chin Med. 2013;41:131. doi: 10.1142/S0192415X13500109
    Species: Human
    Sample type: Conditioned Media
  4. Mamlouk, O. et al. Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes. Int J Radiat Biol. 2012 Dec;88(12):1019-27. doi: 10.3109/09553002.2012.702297. Epub 2012 Jul 13
    Species: Human
    Sample type: Conditioned Media
  5. Goktas C., Cosun A., Bicik Z., et al. Evaluating ESWL-induced renal injury based on urinary TNF-?, IL-1?, and IL-6 levels. Urol Res. 2012 Oct;40(5):569-73.
    Species: Human
    Sample type: Urine
  6. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-?)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample type: Serum
  7. Liu, Man-Hai, et al. "The protective effects of coumestrol against amyloid-beta peptide-and lipopolysaccharide-induced toxicity on mice astrocytes." Neurological research 33.6 (2011): 663-672.
    Species: Human
    Sample type: Conditioned Media
  8. Immunomonitorization of cytokines: renal transplant patient into tolerance or rejection?
    Species: Human
    Sample type:
  9. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
    Species: Human
    Sample type:
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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