Human IL-1 beta ELISA

$398.00
ELH-IL1b-1

RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
0.3 pg/ml

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Detection Range
0.3 pg/ml - 100 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Interleukin-1 beta (IL-1 beta) (Catabolin)
Accession Number
Gene Names
IL1B, IL1F2
Gene ID
3553

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL1B

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Zhang L., Wang X., Miao Y., et al. Magnetic ferroferric oxide nanoparticles induce vascular endothelial cell dysfunction and inflammation by disturbing autophagy. J Hazard Mater. 2016 Mar 5;304:186-95. doi: 10.1016/j.jhazmat.2015.10.041
    Species: Human
    Sample Type: Conditioned Media

  2. Ildefonso CJ., Jaime H., Rahman M., et al. Gene Delivery of a Viral Anti Inflammatory Protein to Combat Ocular Inflammation. HUMAN GENE THERAPY 26:59-68 (January 2015). DOI: 10.1089/hum.2014.089
    Species: Human
    Sample Type: Conditioned Media

  3. Ildefonso CJ., Jaime H., Rahman M., et al. Gene Delivery of a Viral Anti Inflammatory Protein to Combat Ocular Inflammation. HUMAN GENE THERAPY 26:59-68 (January 2015). DOI: 10.1089/hum.2014.089
    Species: Human
    Sample Type: Tissue Lysate

  4. Filkova M., Vernerova Z., Hulejova H., et al. Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis. Cytokine. 2015 May;73(1):36-43. doi: 10.1016/j.cyto.2015.01.019.
    Species: Human
    Sample Type: Conditioned Media

  5. Diabate M., Muron P., Garcia E., et al. Escherichia coli α-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia. PLoS Pathog. 2015 Mar 17;11(3):e1004732. doi: 10.1371/journal.ppat.1004732.
    Species: Human
    Sample Type: Conditioned Media

  6. Mankad D., Dupuis A., Smile S., et al. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism. 2015; 6: 18. Published online 2015 Mar 21. doi: 10.1186/s13229-015-0010-7
    Species: Human
    Sample Type: Plasma

  7. Jiang H., Ling Z., Zhang Y., et al. Altered fecal microbiota composition in patients with major depressive disorder. Brain Behav Immun. 2015 Aug;48:186-94. doi: 10.1016/j.bbi.2015.03.016
    Species: Human
    Sample Type: Serum

  8. Ildefonso C., Jaime H., Biswal M., et al. Gene therapy with the caspase activation and recruitment domain reduces the ocular inflammatory response. Mol Ther. 2015 May;23(5):875-84. doi: 10.1038/mt.2015.30.
    Species: Human
    Sample Type: Conditioned Media

  9. Gupta M., Chari S. Predictive value of inflammatory Cytokines in preeclampsia. International Journal of Biomedical and Advance Research 2015; 6(04): 334-338.
    Species: Human
    Sample Type: Serum

  10. Zhang X., Yu C., Liu J., et al. Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells. Evidence-Based Complementary and Alternative MedicineVolume 2015, Article ID 518639, 8 pages http://dx.doi.org/10.1155/2015/518639
    Species: Human
    Sample Type: Conditioned Media

  11. Ye J., Xu Y-F., Lou L-X., et al. Anti-inflammatory effects of hinokitiol on human corneal epithelial cells: an in vitro study. Eye (Lond). 2015 Jul;29(7):964-71. doi: 10.1038/eye.2015.62
    Species: Human
    Sample Type: Conditioned Media

  12. Zhang H., Zhao C., Wang S., et al. Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells. J Transl Med. 2015 Jun 4;13:178. doi: 10.1186/s12967-015-0536-7.
    Species: Human
    Sample Type: Conditioned Media

  13. Ansari MA, Dutta S, Veettil MV, Dutta D, Iqbal J, Kumar B, et al. (2015) Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses. PLoS Pathog 11(7): e1005019. doi:10.1371/journal.ppat.1005019
    Species: Human
    Sample Type: Conditioned Media

  14. Pulikkotil S., Nath S. Effects of curcumin on crevicular levels of IL-1β and CCL28 in experimental gingivitis. Aust Dent J. 2015 Sep;60(3):317-27. doi: 10.1111/adj.12340
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  15. Yiu E., Chan K., Li N., et al. Wound-healing effect of acupuncture for treating phonotraumatic vocal pathologies: A cytokine study. Laryngoscope. 2016 Jan;126(1):E18-22. doi: 10.1002/lary.25483.
    Species: Human
    Sample Type: Laryngeal Secretions

  16. Wu X., Zheng B., Jin L., et al. Chinese medicine Tongxinluo reduces atherosclerotic lesion by attenuating oxidative stress and inflammation in microvascular endothelial cells. Int J Clin Exp Pathol. 2015; 8(6): 6323-6333.
    Species: Human
    Sample Type: Conditioned Media

  17. Wang P., Ma X., He Y., et al. Effect of p38 mitogen-activate protein kinase on MUC5AC protein expression of bile duct epithelial cells in hepatolithiasis patients.

  18. Abdallah M., et al. Serum Th1/Th2 and Macrophage Lineage Cytokines in Leprosy; Correlation with Circulating CD4+ CD25highFoxP3+ T-regs Cells. Experimental Dermatology, August 2014 Accepted Article, doi:10.1111/exd.12529
    Species: Human
    Sample Type: Serum

  19. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
    Species: Human
    Sample Type: Conditioned Media

  20. Bishayi B., et al. Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes. Inflammation August 2014, Epub ahead of print. DOI: 10.1007/s10753-014-9991-1
    Species: Human
    Sample Type: Conditioned Media

  21. Chowdhurya I.H., et al. Alteration of serum inflammatory cytokines in active pulmonarytuberculosis following anti-tuberculosis drug therapy. Molecular Immunology 62 (2014) 159-168
    Species: Human
    Sample Type: Serum

  22. Duan J, Yu Y, Yu Y, et al. Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway. International Journal of Nanomedicine 2014;9:5131-5141. doi:10.2147/IJN.S71074.
    Species: Human
    Sample Type: Conditioned Media

  23. Leyh et al.: Subchondral bone influenceschondrogenic differentiation and collagen production of human bonemarrow-derived mesenchymal stem cells and articular chondrocytes.Arthritis Research & Therapy 2014 16:453. doi:10.1186/s13075-014-0453-9
    Species: Human
    Sample Type: Conditioned Media

  24. Scherer O, Steinmetz H, Kaether C, Weinigel C, Barz D,Kleinert H, Menche D, M¨uller R, Pergola C, Werz O, Targeting V-ATPase in primaryhuman monocytes by archazolid potently represses the classical secretion of cytokinesdue to accumulation at the endoplasmic reticulum, Biochemical Pharmacology (2014), http://dx.doi.org/10.1016/j.bcp.2014.07.028
    Species: Human
    Sample Type: Cell Lysate

  25. Shivaprasad K., Kumar M., Dutta D., et al. INCREASED SOLUBLE TNF RECEPTOR-1 AND GLUTATHIONE PEROXIDASE MAY PREDICT CAROTID INTIMA MEDIA THICKNESS IN FEMALES WITH CUSHING’S SYNDROME
    Species: Human
    Sample Type: Serum

  26. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-β, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62
    Species: Human
    Sample Type: Serum

  27. Ertugrul AS, Sahin H, Dikilitas A, et al. Comparison of CCL28, interleukin-8, interleukin-1β and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis. J Periodont Res. 2013 Feb;48(1):44-51.
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  28. He T, Wu W, Huang Y, et al. Multiple biomarkers analysis for the early detection of prosthetic aseptic loosening of hip arthroplasty. Int Orthop. 2013 Jun;37(6):1025-1031.
    Species: Human
    Sample Type: Plasma

  29. Verma P, Dalal K. Serum cartilage oligomeric matrix protein (COMP) in knee osteoarthritis: A novel diagnostic and prognostic biomarker. J Orthop Res. 2013 Jul;31(7):999-1006.
    Species: Human
    Sample Type: Serum

  30. Filková M, Hulejová H, Kuncová K, et al. Resistin in idiopathic inflammatory myopathies. Arthritis Research & Therapy. 2012;14(3):R111. doi:10.1186/ar3836.
    Species: Human
    Sample Type: Serum

  31. Kraus D, Winter J, Jepsen S, Jäger A, Meyer R, Deschner J. Interactions of Adiponectin and Lipopolysaccharide from Porphyromonas gingivalis on Human Oral Epithelial Cells. Heimesaat MM, ed. PLoS ONE 2012;7(2):e30716. doi:10.1371/journal.pone.0030716.
    Species: Human
    Sample Type: Conditioned Media

  32. Mamlouk, O. et al. Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes. Int J Radiat Biol. 2012 Dec;88(12):1019-27. doi: 10.3109/09553002.2012.702297. Epub 2012 Jul 14
    Species: Human
    Sample Type: Conditioned Media

  33. Liou H., Shih C., Chao Y., et al. Inflammatory response to colloids compared to crystalloid priming in cardiac surgery patients with cardiopulmonary bypass. Chin J Physiol. 2012 Jun 30;55(3):210-8. doi: 10.4077/CJP.2012.AMM122.
    Species: Human
    Sample Type: Serum

  34. Proksch S., Steinberg T., Keller C., et al. Human saliva exposure modulates bone cell performance in vitro. Clin Oral Investig. 2012 Feb;16(1):69-77. doi: 10.1007/s00784-010-0506-7.
    Species: Human
    Sample Type: Conditioned Media

  35. Pooja S., Chaudhary P., Nayak L., et al. Polymorphic variations in IL-1β, IL-6 and IL-10 genes, their circulating serum levels and breast cancer risk in Indian women. Cytokine. 2012 Oct;60(1):122-8. doi: 10.1016/j.cyto.2012.06.241.
    Species: Human
    Sample Type: Serum

  36. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
    Species: Human

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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