Human IL-1 beta ELISA

RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$361.80
You save: $40.20 (10%)
ELH-IL1b-1
+

Antigen Information

Accession Number:
  • P01584
Gene ID:
  • 3553
Gene Symbols:
  • IL1B
  • IL1F2
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta) (Catabolin)
Target Species:
  • Human

Assay Format

Detection Range:
0.3 pg/ml - 100 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
0.3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Siqi Feng, Ya Su, Li Luo, Jing Fengchuan, Qijian Yi, Serum levels of C1q/tumor necrosis factor related protein-1 in children with Kawasaki disease, Pediatric Research accepted article preview 23 January 2018; doi: 10.1038/pr.2018.16.
    Species: Human
    Sample type: Serum
  2. Zhang L., Wang X., Miao Y., et al. Magnetic ferroferric oxide nanoparticles induce vascular endothelial cell dysfunction and inflammation by disturbing autophagy. J Hazard Mater. 2016 Mar 5;304:186-95. doi: 10.1016/j.jhazmat.2015.10.041
    Species: Human
    Sample type: Conditioned Media
  3. Ildefonso CJ., Jaime H., Rahman M., et al. Gene Delivery of a Viral Anti Inflammatory Protein to Combat Ocular Inflammation. HUMAN GENE THERAPY 26:59-68 (January 2015). DOI: 10.1089/hum.2014.089
    Species: Human
    Sample type: Tissue Lysate
  4. Filkova M., Vernerova Z., Hulejova H., et al. Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis. Cytokine. 2015 May;73(1):36-43. doi: 10.1016/j.cyto.2015.01.019.
    Species: Human
    Sample type: Conditioned Media
  5. Diabate M., Muron P., Garcia E., et al. Escherichia coli ?-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia. PLoS Pathog. 2015 Mar 17;11(3):e1004732. doi: 10.1371/journal.ppat.1004732.
    Species: Human
    Sample type: Conditioned Media
  6. Mankad D., Dupuis A., Smile S., et al. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism. 2015; 6: 18. Published online 2015 Mar 21. doi: 10.1186/s13229-015-0010-7
    Species: Human
    Sample type: Plasma
  7. Jiang H., Ling Z., Zhang Y., et al. Altered fecal microbiota composition in patients with major depressive disorder. Brain Behav Immun. 2015 Aug;48:186-94. doi: 10.1016/j.bbi.2015.03.016
    Species: Human
    Sample type: Serum
  8. Ildefonso C., Jaime H., Biswal M., et al. Gene therapy with the caspase activation and recruitment domain reduces the ocular inflammatory response. Mol Ther. 2015 May;23(5):875-84. doi: 10.1038/mt.2015.30.
    Species: Human
    Sample type: Conditioned Media
  9. Gupta M., Chari S. Predictive value of inflammatory Cytokines in preeclampsia. International Journal of Biomedical and Advance Research 2015; 6(04): 334-338.
    Species: Human
    Sample type: Serum
  10. Zhang X., Yu C., Liu J., et al. Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells. Evidence-Based Complementary and Alternative MedicineVolume 2015, Article ID 518639, 8 pages http://dx.doi.org/10.1155/2015/518639
    Species: Human
    Sample type: Conditioned Media
Worked well for cell culture supernatant.

University of South Florida
I bought the human IL1b ELISA kit in October, 2018. It's perfect for cell culture supernatants.

Augusta University
Excellent

University of the Pacific
I used this kit on conditioned media from cardiac progenitor cells. I didn't dilute my samples and it worked very well.

Emory University
I used it with human corneal epithelial cell supernatant treated with various stimulus. It's very important for me to have high quality and sensitivity. This product provides that. Also very easy to use and follow the instructions on the protocol.

University of Illinois at Chicago
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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