Human IL-10 ELISA

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RayBio® Human IL-10 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-IL10-1
+

Antigen Information

Accession Number:
  • P22301
Gene ID:
  • 3586
Gene Symbol:
  • IL10
Protein Name & Synonyms:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 150 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
    Species: Human
    Sample type: Plasma
  2. Mankad D., Dupuis A., Smile S., et al. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism. 2015; 6: 18. Published online 2015 Mar 21. doi: 10.1186/s13229-015-0010-7
    Species: Human
    Sample type: Plasma
  3. Zeng J., Xiang W., Lin D., et al. Elevated HMGB1-related interleukin-6 is associated with dynamic responses of monocytes in patients with active pulmonary tuberculosis. Int J Clin Exp Pathol. 2015 Feb 1;8(2):1341-53.
    Species: Human
    Sample type: Sputum
  4. Karaduman B., Uraz A., Altan G., et al. Changes of tumor necrosis factor-?, interleukin-10, and tartrate-resistant acid phosphatase5b in the crevicular fluid in relation to orthodontic movement European Journal of Inflammation April 2015 vol. 13 no. 1 3-13. doi: 10.1177/1721727X15573917
    Species: Human
    Sample type: Gingival Crevicular Fluid
  5. Romanets-Korbut O., Kovalevska L., Seya T., et al. Measles virus hemagglutinin triggers intracellular signaling in CD150-expressing dendritic cells and inhibits immune response. Cellular & Molecular Immunology advance online publication 15 June 2015; doi: 10.1038/cmi.2015.55
    Species: Human
    Sample type: Conditioned Media
  6. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample type: Conditioned Media
  7. Jahromi S., Mahesh P., Jayaraj B., et al. IL-10 and IL-17F Promoter Single Nucleotide Polymorphism and Asthma: A Case-Control Study in South India. Lung (2015) 193:739-747 DOI 10.1007/s00408-015-9753-3
    Species: Human
    Sample type: Serum
  8. Yiu E., Chan K., Li N., et al. Wound-healing effect of acupuncture for treating phonotraumatic vocal pathologies: A cytokine study. Laryngoscope. 2016 Jan;126(1):E18-22. doi: 10.1002/lary.25483.
    Species: Human
    Sample type: Laryngeal Secretions
  9. Odewumi C., Latinwo L., Sinclair A., et al. Effect of cadmium on the expression levels of interleukin-1? and interleukin-10 cytokines in human lung cells. Mol Med Rep. 2015 Nov;12(5):6422-6. doi: 10.3892/mmr.2015.4316.
    Species: Human
    Sample type: Conditioned Media
  10. Attia EAS., et al. Serum Th17 cytokines in leprosy: correlation with circulatingCD4+ CD25highFoxP3+ T-regs cells, as well as down regulatory cytokines. Arch Dermatol Res. Published online July 2014. DOI 10.1007/s00403-014-1486-2
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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