Human IL-10 ELISA

$398.00
ELH-IL10-1

RayBio® Human IL-10 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
1 pg/ml

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Detection Range
1 pg/ml - 150 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Accession Number
Gene Names
IL10
Gene ID
3586

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL10

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Mankad D., Dupuis A., Smile S., et al. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism. 2015; 6: 18. Published online 2015 Mar 21. doi: 10.1186/s13229-015-0010-7
    Species: Human
    Sample Type: Plasma

  2. Zeng J., Xiang W., Lin D., et al. Elevated HMGB1-related interleukin-6 is associated with dynamic responses of monocytes in patients with active pulmonary tuberculosis. Int J Clin Exp Pathol. 2015 Feb 1;8(2):1341-53.
    Species: Human
    Sample Type: Plasma

  3. Zeng J., Xiang W., Lin D., et al. Elevated HMGB1-related interleukin-6 is associated with dynamic responses of monocytes in patients with active pulmonary tuberculosis. Int J Clin Exp Pathol. 2015 Feb 1;8(2):1341-53.
    Species: Human
    Sample Type: Conditioned Media

  4. Zeng J., Xiang W., Lin D., et al. Elevated HMGB1-related interleukin-6 is associated with dynamic responses of monocytes in patients with active pulmonary tuberculosis. Int J Clin Exp Pathol. 2015 Feb 1;8(2):1341-53.
    Species: Human
    Sample Type: Sputum

  5. Karaduman B., Uraz A., Altan G., et al. Changes of tumor necrosis factor-α, interleukin-10, and tartrate-resistant acid phosphatase5b in the crevicular fluid in relation to orthodontic movement European Journal of Inflammation April 2015 vol. 13 no. 1 3-13. doi: 10.1177/1721727X15573917
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  6. Romanets-Korbut O., Kovalevska L., Seya T., et al. Measles virus hemagglutinin triggers intracellular signaling in CD150-expressing dendritic cells and inhibits immune response. Cellular & Molecular Immunology advance online publication 15 June 2015; doi: 10.1038/cmi.2015.55
    Species: Human
    Sample Type: Conditioned Media

  7. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample Type: Conditioned Media

  8. Jahromi S., Mahesh P., Jayaraj B., et al. IL-10 and IL-17F Promoter Single Nucleotide Polymorphism and Asthma: A Case-Control Study in South India. Lung (2015) 193:739-747 DOI 10.1007/s00408-015-9753-3
    Species: Human
    Sample Type: Serum

  9. Yiu E., Chan K., Li N., et al. Wound-healing effect of acupuncture for treating phonotraumatic vocal pathologies: A cytokine study. Laryngoscope. 2016 Jan;126(1):E18-22. doi: 10.1002/lary.25483.
    Species: Human
    Sample Type: Laryngeal Secretions

  10. Odewumi C., Latinwo L., Sinclair A., et al. Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells. Mol Med Rep. 2015 Nov;12(5):6422-6. doi: 10.3892/mmr.2015.4316.
    Species: Human
    Sample Type: Conditioned Media

  11. Attia EAS., et al. Serum Th17 cytokines in leprosy: correlation with circulatingCD4+ CD25highFoxP3+ T-regs cells, as well as down regulatory cytokines. Arch Dermatol Res. Published online July 2014. DOI 10.1007/s00403-014-1486-2
    Species: Human
    Sample Type: Serum

  12. Chen Z., et al. IL-6, IL-10 and IL-13 are associated with pathogenesis in children with Enterovirus 71 infection. Int J Clin Exp Med 2014;7(9):2718-2723
    Species: Human
    Sample Type: Serum

  13. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
    Species: Human
    Sample Type: Tissue Lysate

  14. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample Type: Tissue Lysate

  15. Santarelli E., Gonnella R., Giovenale G., Cuomo L., Capobianchi A., et al. STAT3 activation by KSHV correlates with IL-10, IL-6 and IL-23 release and an autophagic block in dendritic cells. Scientific Reports, 2014.4 article 4241, doi:10.1038/srep04241
    Species: Human
    Sample Type: Conditioned Media

  16. Wang, F., He, D., et al. Chemokine expression in diverse nonimmediate drug hypersensitivity reactions: focus on thymus activation-regulated chemokine, cutaneous T-cell-attracting chemokine, and interleukin-10. Annals of Allergy, Asthma & Immunology 2014. Epub Ahead of Print DOI: 10.1016/j.anai.2014.05.006
    Species: Human
    Sample Type: Serum

  17. Chen J., Shen B., Jiang Y., Jun L., Zhu M., Chen B., Liu C. Analysis of immunoglobulin-like transcripts (ILTs) in lymphocytes with sHLA-G and IL10 from SLE patients. Clinical and Experimental Medicine, 2013. Vol 13; Issue 2, 135-142.
    Species: Human
    Sample Type: Plasma

  18. Liou H-L, Shih C-C, Chung K-C, Chen HI. Comparison of the Effect of Intensive Versus Conventional Insulinotherapy in Patients with Cardiac Surgery after Cardiopulmonary Bypass. Chin J Physiol. 2013 Apr 30;56(2).
    Species: Human
    Sample Type: Serum

  19. Rocher, Crystal, and Dinender K. Singla. "SMAD-PI3K-Akt-mTOR Pathway Mediates BMP-7 Polarization of Monocytes into M2 Macrophages." PLoS One. NCBI, 20 Dec. 2013. Web.
    Species: Human
    Sample Type: Conditioned Media

  20. Garufi A, Pistritto G, Ceci C, et al. Targeting COX-2/PGE2 Pathway in HIPK2 Knockdown Cancer Cells: Impact on Dendritic Cell Maturation. Tasken K, ed. PLoS ONE 2012;7(11):e48342. doi:10.1371/journal.pone.0048342.
    Species: Human
    Sample Type: Conditioned Media

  21. Wang XS, Williams LA, Krishnan S, et al. Serum sTNFR1, IL6, and the Development of Fatigue in Patients with Gastrointestinal Cancer Undergoing Chemoradiation Therapy. Brain, Behavior, and Immunity. 2012;26(5):699-705. doi:10.1016/j.bbi.2011.12.007.
    Species: Human
    Sample Type: Serum

  22. Rocher C., Singla R., Singal P., et al. Bone morphogenetic protein 7 polarizes THP-1 cells into M2 macrophages. Can J Physiol Pharmacol. 2012 Jul;90(7):947-51. doi: 10.1139/y2012-102.
    Species: Human
    Sample Type: Conditioned Media

  23. Liou H., Shih C., Chao Y., et al. Inflammatory response to colloids compared to crystalloid priming in cardiac surgery patients with cardiopulmonary bypass. Chin J Physiol. 2012 Jun 30;55(3):210-8. doi: 10.4077/CJP.2012.AMM122.
    Species: Human
    Sample Type: Serum

  24. Pooja S., Chaudhary P., Nayak L., et al. Polymorphic variations in IL-1β, IL-6 and IL-10 genes, their circulating serum levels and breast cancer risk in Indian women. Cytokine. 2012 Oct;60(1):122-8. doi: 10.1016/j.cyto.2012.06.241.
    Species: Human
    Sample Type: Serum

  25. Abu Alshamat E., Al-Okla S., Soukkarieh C., kweider M. Human chorionic gonadotrophin (hCG) enhances immunity against L. tropica by stimulating human macrophage functions. Parasite Immunol. 2012 Oct;34(10):449-54. doi: 10.1111/j.1365-3024.2012.01368.x.
    Species: Human
    Sample Type: Conditioned Media

  26. Youssef D., Elshal A., Abo Elazem A. Assessment of immune status in relation to vitamin D levels in children on regular hemodialysis. Saudi J Kidney Dis Transpl. 2012 Mar;23(2):267-73.
    Species: Human
    Sample Type: Serum

  27. Bose, Purabi Deka, et al. "High viral load and deregulation of the progesterone receptor signaling pathway: association with hepatitis E-related poor pregnancy outcome." Journal of hepatology 54.6 (2011): 1107-1113.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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