Human IL-13 ELISA

RayBio® Human IL-13 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-IL13-1
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Antigen Information

Accession Number:
  • P35225
Gene ID:
  • 3596
Gene Symbols:
  • IL13
  • NC30
Protein Name & Synonyms:
Interleukin-13 (IL-13)
Target Species:
  • Human

Assay Format

Detection Range:
0.15 pg/ml - 40 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
0.15 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Gao S., Zhou J., Liu N., et al. Curcumin induces M2 macrophage polarization by secretion IL-4 and/or IL-13. J Mol Cell Cardiol. 2015 Aug;85:131-9. doi: 10.1016/j.yjmcc.2015.04.025.
    Species: Human
    Sample type: Conditioned Media
  2. Chen Z., et al. IL-6, IL-10 and IL-13 are associated with pathogenesis in children with Enterovirus 71 infection. Int J Clin Exp Med 2014;7(9):2718-2723
    Species: Human
    Sample type: Serum
  3. Akelma AZ, Cizmeci MN, Kanburoglu MK, Bozhkaya D, Catal F, et al. Elevated level of serum osteopontin in school-age children with asthma. Allergol Immunopathol (Madr). 2013; Published online ahead of print, April 8, 2013. doi:10.1016/j.aller.2013.01.009
    Species: Human
    Sample type: Serum
  4. Hashad RA, Hamed NA, El Gharabawy MM, et al. Interleukins 12 and 13 levels among beta-thalassaemia major patients. East Mediterr Health J. 2013;19(2):181-185.
    Species: Human
    Sample type: Conditioned Media
  5. Barderas R., Bartolome R., Fernandez-Acenero MJ., et al. High expression of IL-13 receptor ?2 in colorectal cancer is associated with invasion, liver metastasis, and poor prognosis.. Cancer Res. 2012 Jun 1;72(11):2780-90. doi: 10.1158/0008-5472.CAN-11-4090
    Species: Human
    Sample type: Conditioned Media
  6. Nagayoshi, Masaru, et al. "Inhalation of Stachybotrys chartarum evokes pulmonary arterial remodeling in mice, attenuated by Rho-kinase inhibitor." Mycopathologia 172.1 (2011): 5-15.
    Species: Human
    Sample type: BAL Fluid
  7. Iikura, Katsuhito, et al. "Peripheral blood mononuclear cells from patients with bronchial asthma show impaired innate immune responses to rhinovirus in vitro." International archives of allergy and immunology 155.Suppl. 1 (2011): 27-33.
    Species: Human
    Sample type: Conditioned Media
  8. RA, Harfoush, and Elgharabawy MM. "Levels of interleukins 12 (IL-12) and 13 (IL-13), hepatitis B and C serology, and blood cultures among acute myeloid leukemia (AML) patients in Egypt." Journal of Venomous Animals and Toxins including Tropical Diseases 17.3 (2011): 293-299.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.