Human IL-17 ELISA

RayBio® Human IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-IL17-1
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Antigen Information

Accession Number:
  • Q16552
Gene ID:
  • 3605
Gene Symbols:
  • CTLA8
  • IL17
  • IL17A
Protein Name & Synonyms:
Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
Target Species:
  • Human

Assay Format

Detection Range:
80 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
80 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Talaat RM., Mohamed SF., Bassyouni IH., Raouf AA. Th1/Th2/Th17/Treg cytokine imbalance in systemic lupus erythematosus (SLE) patients: Correlation with disease activity. Cytokine. 2015 Apr;72(2):146-53. doi: 10.1016/j.cyto.2014.12.027.
    Species: Human
    Sample type: Plasma
  2. Chitrapriya M., Rao S., Lavu V. Interleukin-17 and interleukin-18 levels in different stages of inflammatory periodontal disease. J Indian Soc Periodontol. 2015 Jan-Feb;19(1):14-7. doi: 10.4103/0972-124X.145798.
    Species: Human
    Sample type: Tissue Lysate
  3. Talaat RM., Sidek A., Mosalem A., Kholief A. Effect of bisphosphonates treatment on cytokine imbalance between TH17 and Treg in osteoporosis. Inflammopharmacology. 2015 Jun;23(2-3):119-25. doi: 10.1007/s10787-015-0233-4.
    Species: Human
    Sample type: Plasma
  4. Yi H., Yu C., Zhang H., et al. Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-?B signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets. Int Immunopharmacol. 2015 Sep;28(1):61-9. doi: 10.1016/j.intimp.2015.05.034
    Species: Porcine
    Sample type: Serum
  5. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample type: Conditioned Media
  6. Hussein A A., Al- Marsome H T. and Al-Musawi H N. (2015); Role of IL-17 and IL-27 in Cutaneous Leishmaniasis Int. J. of Adv. Res. 3 (6). 267-170] (ISSN 2320-5407)
    Species: Human
    Sample type: Serum
  7. Elhewala A., Soliman S., Labib A., et al. Interleukin-17 level in rheumatoid arthritis patients and its relation to disease activity: a clinical and ultrasound study. Egyptian Rheumatology and Rehabilitation Year : 2015 | Volume : 42 | Issue : 4 | Page : 183-187 DOI: 10.4103/1110-161X.168164
    Species: Human
    Sample type: Serum
  8. Tofiq D., Merza R. ASSESSMENT OF THE ROLE OF IL-17A IN RHEUMATOID ARTHRITIS PATIENTS; IN SULAYMANIYAH GOVERNORATE. European Scientific Journal, Vol 11 No 33, 2015
    Species: Human
    Sample type: Serum
  9. Attia EAS., et al. Serum Th17 cytokines in leprosy: correlation with circulatingCD4+ CD25highFoxP3+ T-regs cells, as well as down regulatory cytokines. Arch Dermatol Res. Published online July 2014. DOI 10.1007/s00403-014-1486-2
    Species: Human
    Sample type: Serum
  10. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
    Species: Human
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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