Human IL-17 ELISA

$398.00
ELH-IL17-1

RayBio® Human IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
80 pg/ml

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Detection Range
80 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
Accession Number
Gene Names
IL17A, CTLA8, IL17
Gene ID
3605

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL17A

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Talaat RM., Mohamed SF., Bassyouni IH., Raouf AA. Th1/Th2/Th17/Treg cytokine imbalance in systemic lupus erythematosus (SLE) patients: Correlation with disease activity. Cytokine. 2015 Apr;72(2):146-53. doi: 10.1016/j.cyto.2014.12.027.
    Species: Human
    Sample Type: Plasma

  2. Chitrapriya M., Rao S., Lavu V. Interleukin-17 and interleukin-18 levels in different stages of inflammatory periodontal disease. J Indian Soc Periodontol. 2015 Jan-Feb;19(1):14-7. doi: 10.4103/0972-124X.145798.
    Species: Human
    Sample Type: Tissue Lysate

  3. Talaat RM., Sidek A., Mosalem A., Kholief A. Effect of bisphosphonates treatment on cytokine imbalance between TH17 and Treg in osteoporosis. Inflammopharmacology. 2015 Jun;23(2-3):119-25. doi: 10.1007/s10787-015-0233-4.
    Species: Human
    Sample Type: Plasma

  4. Yi H., Yu C., Zhang H., et al. Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κ signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets. Int Immunopharmacol. 2015 Sep;28(1):61-9. doi: 10.1016/j.intimp.2015.05.034
    Species: Porcine
    Sample Type: Serum

  5. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample Type: Conditioned Media

  6. Hussein A A., Al- Marsome H T. and Al-Musawi H N. (2015); Role of IL-17 and IL-27 in Cutaneous Leishmaniasis Int. J. of Adv. Res. 3 (6). 267-170] (ISSN 2320-5407)
    Species: Human
    Sample Type: Serum

  7. Elhewala A., Soliman S., Labib A., et al. Interleukin-17 level in rheumatoid arthritis patients and its relation to disease activity: a clinical and ultrasound study. Egyptian Rheumatology and Rehabilitation Year : 2015 | Volume : 42 | Issue : 4 | Page : 183-187 DOI: 10.4103/1110-161X.168164
    Species: Human
    Sample Type: Serum

  8. Tofiq D., Merza R. ASSESSMENT OF THE ROLE OF IL-17A IN RHEUMATOID ARTHRITIS PATIENTS; IN SULAYMANIYAH GOVERNORATE. European Scientific Journal, Vol 11 No 33, 2015

  9. Attia EAS., et al. Serum Th17 cytokines in leprosy: correlation with circulatingCD4+ CD25highFoxP3+ T-regs cells, as well as down regulatory cytokines. Arch Dermatol Res. Published online July 2014. DOI 10.1007/s00403-014-1486-2
    Species: Human
    Sample Type: Serum

  10. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
    Species: Human
    Sample Type: Tissue Lysate

  11. Hakemi M., et al. Positive and Negative Regulation of Th17 Cell Differentiation: Evaluating The Impact of RORC2. CELL JOURNAL(Yakhteh), Vol 16, No 3, Autumn 2014.
    Species: Human
    Sample Type: Conditioned Media

  12. Petek-Balci B., et al. Predictive value of early serum cytokine changes onlong-term interferon beta-1a efficacy in multiple sclerosis. International Journal of Neuroscience, 2014; Early Online July 2014. DOI: 10.3109/00207454.2014.939747
    Species: Human
    Sample Type: Serum

  13. Akelma AZ, Cizmeci MN, Kanburoglu MK, Bozhkaya D, Catal F, et al. Elevated level of serum osteopontin in school-age children with asthma. Allergol Immunop (Madr). 2013; Published online ahead of print, April 8, 2013. doi:10.1016/j.aller.2013.01.009
    Species: Human
    Sample Type: Serum

  14. Association between plasma levels of transforming growth factor-κeta]1, IL-23 and IL-17 and the severity of autism in Egyptian children
    Species: Human
    Sample Type: Plasma

  15. Involvement of corneal epithelial cells in the Th17 response in an in vitro bacterial inflammation model
    Species: Human
    Sample Type: Conditioned Media

  16. Karadag AS., Ertugrul DT., Bilgil SG., et al. Immunoregulatory effects of isotretinoin in patients with acne. Br J Dermatol. 2012 Aug;167(2):433-5. doi: 10.1111/j.1365-2133.2012.10949.x.
    Species: Human
    Sample Type: Serum

  17. Kurtuncu M., Tuzun E., Turkoglu R., et al. Effect of short-term interferon-β treatment on cytokines in multiple sclerosis: significant modulation of IL-17 and IL-23. Cytokine. 2012 Aug;59(2):400-2. doi: 10.1016/j.cyto.2012.05.004.
    Species: Human
    Sample Type: Serum

  18. Ulusoy C., Tuzun E., Kurtuncu M., et al. Comparison of the cytokine profiles of patients with neuronal-antibody-associated central nervous system disorders. Int J Neurosci. 2012 Jun;122(6):284-9. doi: 10.3109/00207454.2011.648762.
    Species: Human
    Sample Type: Serum

  19. Raychaudhuri S., Raychauduri S., Genovese M. IL-17 receptor and its functional significance in psoriatic arthritis. Mol Cell Biochem. 2012 Jan;359(1-2):419-29. doi: 10.1007/s11010-011-1036-6.
    Species: Human
    Sample Type: Synovial Fluid

  20. Wolfram D., Rabensteiner E., Grundtman C., et al. T regulatory cells and TH17 cells in peri-silicone implant capsular fibrosis. Plast Reconstr Surg. 2012 Feb;129(2):327e-337e. doi: 10.1097/PRS.0b013e31823aeacf.
    Species: Human
    Sample Type: Conditioned Media

  21. Li Y., Jiang L., Zhang S., et al. Methotrexate attenuates the Th17/IL-17 levels in peripheral blood mononuclear cells from healthy individuals and RA patients. Rheumatol Int. 2012 Aug;32(8):2415-22. doi: 10.1007/s00296-011-1867-1.
    Species: Human
    Sample Type: Conditioned Media

  22. Sibilano R., Frossi B., Calvaruso M., et al. The aryl hydrocarbon receptor modulates acute and late mast cell responses. J Immunol. 2012 Jul 1;189(1):120-7. doi: 10.4049/jimmunol.1200009.
    Species: Human
    Sample Type: Conditioned Media

  23. Kouam S., Kusari S., Lamshoft M., et al. Sapelenins G-J, acyclic triterpenoids with strong anti-inflammatory activities from the bark of the Cameroonian medicinal plant Entandrophragma cylindricum. Phytochemistry. 2012 Nov;83:79-86. doi: 10.1016/j.phytochem.2012.06.004.
    Species: Human
    Sample Type: Conditioned Media

  24. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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