Human IL-17 ELISA

RayBio® Human IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
80 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
80 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q16552
Gene ID:
  • 3605
Gene Symbols:
  • CTLA8
  • IL17
  • IL17A
Protein Name & Synonyms:
Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Chatterjee S, Bhattcharjee D, Misra S, Saha A, Bhattacharyya NP, Ghosh A. Increase in MEG3, MALAT1, NEAT1 significantly predicts the clinical parameters in patients with rheumatoid arthritis. Per Med. 2020 Nov;17(6):445-457. doi: 10.2217/pme-2020-0009. Epub 2020 Oct 7. PMID: 33026292.
    Species: Human
    Sample type: Serum
  2. Bayrak Degirmenci P, Aksun S, Altin Z, Bilgir F, Arslan IB, Colak H, Ural B, Solakoglu Kahraman D, Diniz G, Ozdemir B, K?rmaz C. Allergic Rhinitis and Its Relationship with IL-10, IL-17, TGF-?, IFN-?, IL 22, and IL-35. Dis Markers. 2018 Mar 6;2018:9131432. doi: 10.1155/2018/9131432. PMID: 29692871; PMCID: PMC5859791.
    Species: Human
    Sample type: Plasma
  3. Liu J, Lin J, He S, Wu C, Wang B, Liu J, Duan Y, Liu T, Shan S, Yang K, Dong N. Transgenic overexpression of IL-37 protects against atherosclerosis and strengthens plaque stability. Cellular Physiology and Biochemistry. 2018;45(3):1034-50.
    Species: human
    Sample type: Conditioned Media
  4. Fu X., Cai J., Huang Q., et al. Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy. Oncotarget. 2017 Nov 24; 8(60): 102191–102198, doi: 10.18632/oncotarget.22188
    Species: Human
    Sample type: Conditioned Media
  5. Budamakuntla, L., et al. "IL 17 Cytokine in Psoriasis: Before and After Methotrexate and NBUVB Phototherapy: A Longitudinal Study." Clin Dermatol Res J 2 1 (2017): 2.
    Species: Human
    Sample type: Serum
  6. Talaat RM., Mohamed SF., Bassyouni IH., Raouf AA. Th1/Th2/Th17/Treg cytokine imbalance in systemic lupus erythematosus (SLE) patients: Correlation with disease activity. Cytokine. 2015 Apr;72(2):146-53. doi: 10.1016/j.cyto.2014.12.027.
    Species: Human
    Sample type: Plasma
  7. Chitrapriya M., Rao S., Lavu V. Interleukin-17 and interleukin-18 levels in different stages of inflammatory periodontal disease. J Indian Soc Periodontol. 2015 Jan-Feb;19(1):14-7. doi: 10.4103/0972-124X.145798.
    Species: Human
    Sample type: Tissue Lysate
  8. Talaat RM., Sidek A., Mosalem A., Kholief A. Effect of bisphosphonates treatment on cytokine imbalance between TH17 and Treg in osteoporosis. Inflammopharmacology. 2015 Jun;23(2-3):119-25. doi: 10.1007/s10787-015-0233-4.
    Species: Human
    Sample type: Plasma
  9. Yi H., Yu C., Zhang H., et al. Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-?B signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets. Int Immunopharmacol. 2015 Sep;28(1):61-9. doi: 10.1016/j.intimp.2015.05.034
    Species: Porcine
    Sample type: Serum
  10. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample type: Conditioned Media

No posts found

Write A Review
U.S. Non-U.S.


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Customers Also Bought Customers Also Bought