Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	23
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Research Area	Cancer, Cancer Immunology, Adaptive Immunity
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 CD137 ILA Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	B7-H2 (ICOS Ligand) Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653 Gene ID: 102723996; 23308 Accession #: O75144 ELISA Kit: RayBio Human B7-H2/ICOS Ligand ELISA	B7-H3 (CD276) Gene Symbol: CD276 B7H3 PSEC0249 UNQ309/PRO352 Gene ID: 80381 Accession #: Q5ZPR3 ELISA Kit: RayBio Human B7-H3 ELISA	B7-H4 Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291 Gene ID: 79679 Accession #: Q7Z7D3	CD200 Gene Symbol: CD200 MOX1 MOX2 My033 Gene ID: 4345 Accession #: P41217 ELISA Kit: RayBio Human CD200 ELISA
CD200 R1 Gene Symbol: CD200R1 CD200R CRTR2 MOX2R OX2R UNQ2522/PRO6015 Gene ID: 131450 Accession #: Q8TD46	CD27 (TNFRSF7) Gene Symbol: CD27 TNFRSF7 Gene ID: 939 Accession #: P26842	HVEM (TNFRSF14/CD270) Gene Symbol: TNFRSF14 HVEA HVEM UNQ329/PRO509 Gene ID: 8764 Accession #: Q92956 ELISA Kit: RayBio Human HVEM ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 CD27L CD27LG TNFSF7 Gene ID: 970 Accession #: P32970	CD28 Gene Symbol: CD28 Gene ID: 940 Accession #: P10747 ELISA Kit: RayBio Human CD28 ELISA
CD40 Ligand (TNFSF5) Gene Symbol: CD40LG CD40L TNFSF5 TRAP Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CD80 (B7-1) Gene Symbol: CD80 CD28LG CD28LG1 LAB7 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80/B7-1 ELISA	CD86 (B7-2) Gene Symbol: CD86 CD28LG2 Gene ID: 942 Accession #: P42081 ELISA Kit: RayBio Human CD86/B7-2 ELISA	CTLA-4 (CD152) Gene Symbol: CTLA4 CD152 Gene ID: 1493 Accession #: P16410	ICOS Gene Symbol: ICOS AILIM Gene ID: 29851 Accession #: Q9Y6W8 ELISA Kit: RayBio Human ICOS ELISA
LAG-3 Gene Symbol: LAG3 FDC Gene ID: 3902 Accession #: P18627 ELISA Kit: RayBio Human LAG3 ELISA	OX40 (TNFRSF4) Gene Symbol: TNFRSF4 TXGP1L Gene ID: 7293 Accession #: P43489 ELISA Kit: RayBio Human OX40/TNFRSF4 ELISA	OX40 Ligand (TNFSF4) Gene Symbol: TNFSF4 TXGP1 Gene ID: 7292 Accession #: P23510 ELISA Kit: RayBio Human OX40L ELISA	PD-1 Gene Symbol: PDCD1 PD1 Gene ID: 5133 Accession #: Q15116 ELISA Kit: RayBio Human PD-1 ELISA	B7-H1 (CD274/PD-L1) Gene Symbol: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 Gene ID: 29126 Accession #: Q9NZQ7 ELISA Kit: RayBio Human B7H1/PD-L1 ELISA
PD-L2 (B7-DC) Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2 Gene ID: 80380 Accession #: Q9BQ51	TIM-1 (KIM-1) Gene Symbol: HAVCR1 KIM1 TIM1 TIMD1 Gene ID: 26762 Accession #: Q96D42 ELISA Kit: RayBio Human TIM-1 ELISA	TIM-3 (KIM-3) Gene Symbol: HAVCR2 TIM3 TIMD3 Gene ID: 84868 Accession #: Q8TDQ0 ELISA Kit: RayBio Human TIM-3 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Immune Checkpoint Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 CD137 ILA Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	B7-H2 (ICOS Ligand) Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653 Gene ID: 102723996; 23308 Accession #: O75144 ELISA Kit: RayBio Human B7-H2/ICOS Ligand ELISA	B7-H3 (CD276) Gene Symbol: CD276 B7H3 PSEC0249 UNQ309/PRO352 Gene ID: 80381 Accession #: Q5ZPR3 ELISA Kit: RayBio Human B7-H3 ELISA	B7-H4 Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291 Gene ID: 79679 Accession #: Q7Z7D3	CD200 Gene Symbol: CD200 MOX1 MOX2 My033 Gene ID: 4345 Accession #: P41217 ELISA Kit: RayBio Human CD200 ELISA
CD200 R1 Gene Symbol: CD200R1 CD200R CRTR2 MOX2R OX2R UNQ2522/PRO6015 Gene ID: 131450 Accession #: Q8TD46	CD27 (TNFRSF7) Gene Symbol: CD27 TNFRSF7 Gene ID: 939 Accession #: P26842	HVEM (TNFRSF14/CD270) Gene Symbol: TNFRSF14 HVEA HVEM UNQ329/PRO509 Gene ID: 8764 Accession #: Q92956 ELISA Kit: RayBio Human HVEM ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 CD27L CD27LG TNFSF7 Gene ID: 970 Accession #: P32970	CD28 Gene Symbol: CD28 Gene ID: 940 Accession #: P10747 ELISA Kit: RayBio Human CD28 ELISA
CD40 Ligand (TNFSF5) Gene Symbol: CD40LG CD40L TNFSF5 TRAP Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CD80 (B7-1) Gene Symbol: CD80 CD28LG CD28LG1 LAB7 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80/B7-1 ELISA	CD86 (B7-2) Gene Symbol: CD86 CD28LG2 Gene ID: 942 Accession #: P42081 ELISA Kit: RayBio Human CD86/B7-2 ELISA	CTLA-4 (CD152) Gene Symbol: CTLA4 CD152 Gene ID: 1493 Accession #: P16410	ICOS Gene Symbol: ICOS AILIM Gene ID: 29851 Accession #: Q9Y6W8 ELISA Kit: RayBio Human ICOS ELISA
LAG-3 Gene Symbol: LAG3 FDC Gene ID: 3902 Accession #: P18627 ELISA Kit: RayBio Human LAG3 ELISA	OX40 (TNFRSF4) Gene Symbol: TNFRSF4 TXGP1L Gene ID: 7293 Accession #: P43489 ELISA Kit: RayBio Human OX40/TNFRSF4 ELISA	OX40 Ligand (TNFSF4) Gene Symbol: TNFSF4 TXGP1 Gene ID: 7292 Accession #: P23510 ELISA Kit: RayBio Human OX40L ELISA	PD-1 Gene Symbol: PDCD1 PD1 Gene ID: 5133 Accession #: Q15116 ELISA Kit: RayBio Human PD-1 ELISA	B7-H1 (CD274/PD-L1) Gene Symbol: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 Gene ID: 29126 Accession #: Q9NZQ7 ELISA Kit: RayBio Human B7H1/PD-L1 ELISA
PD-L2 (B7-DC) Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2 Gene ID: 80380 Accession #: Q9BQ51	TIM-1 (KIM-1) Gene Symbol: HAVCR1 KIM1 TIM1 TIMD1 Gene ID: 26762 Accession #: Q96D42 ELISA Kit: RayBio Human TIM-1 ELISA	TIM-3 (KIM-3) Gene Symbol: HAVCR2 TIM3 TIMD3 Gene ID: 84868 Accession #: Q8TDQ0 ELISA Kit: RayBio Human TIM-3 ELISA

Application Notes

Suggested Application

Kit Components

Human Immune Checkpoint Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

Work perfectly
Rating
I used this kit to detect human immune checkpoints in the serum of healthy donors and cancer patients. The kit is user-friendly, providing clear and specific signals. It was very helpful for my initial screening and provided valuable insights for my further study.
Review by Zion, from The University of Texas MD Anderson Cancer Center, on Jul 22, 2024

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Human Immune Checkpoint Array C1

Product Description

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Target Names

Application Notes

Storage/Stability

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Human Immune Checkpoint Array C1

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Human Immune Checkpoint Array C1

Human Immune Checkpoint Array C1

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Human Immune Checkpoint Array C1

Product Description

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Featured Products

Human Immune Checkpoint Array C1

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

Work perfectly

Ask A Question

Work perfectly

Human Immune Checkpoint Array C1

Human Immune Checkpoint Array C1