Human IP-10 ELISA

RayBio® Human IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-IP10-1
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Antigen Information

Accession Number:
  • P02778
Gene ID:
  • 3627
Gene Symbols:
  • CXCL10
  • INP10
  • SCYB10
Protein Name & Synonyms:
C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) [Cleaved into: CXCL10(1-73)]
Target Species:
  • Human

Assay Format

Detection Range:
8 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
8 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP- 2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample type: Conditioned Media
  2. Gao Q., Camous X., Lu Y., et al. Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies. Neurobiol Aging. 2016 Mar;39:140-6. doi: 10.1016/j.neurobiolaging.2015.12.002.
    Species: Human
    Sample type: Plasma
  3. Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
    Species: Human
    Sample type: Plasma
  4. Gunluoglu G., Seyhan E., Kazancioglu R., et al. Diagnosing latent tuberculosis in immunocompromised patients measuring blood IP-10 production capacity: an analysis of chronic renal failure patients. Intern Med. 2015;54(5):465-72. doi: 10.2169/internalmedicine.54.3245
    Species: Human
    Sample type: Plasma
  5. Marozin S., Altonmonte J., Munoz-Alvarez K., et al. STAT3 inhibition reduces toxicity of oncolytic VSV and provides a potentially synergistic combination therapy for hepatocellular carcinoma. Cancer Gene Ther. 2015 Jun;22(6):317-25. doi: 10.1038/cgt.2015.23.
    Species: Human
    Sample type: Conditioned Media
  6. Kine E., Trine B., Alexander H., et al. Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines. J Interferon Cytokine Res. 2015 Oct;35(10):759-70. doi: 10.1089/jir.2014.0171
    Species: Human
    Sample type: Plasma
  7. Spaks A., Jaunalksne I., Spaka I., et al. Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer. Anticancer Res. 2015 Dec;35(12):6979-83.
    Species: Human
    Sample type: Serum
  8. Hardy GAD, Sieg S, Rodriguez B, et al. Interferon-? is the primary plasma type-I IFN in HIV-1 infection and correlates with immune activation and disease markers. PLoS ONE. 2013;8(2):e56527.
    Species: Human
    Sample type: Plasma
  9. Wu C, Lu X, Wang X, et al. Clinical symptoms, immune factors, and molecular characteristics of an adult male in Shenzhen, China infected with influenza virus H5N1. J Med Virol. 2013 May;85(5):760-768.
    Species: Human
    Sample type: Serum
  10. Bratland, Eirik, Alexander Hellesen, and Eystein S. Husebye. "Induction of CXCL10 chemokine in adrenocortical cells by stimulation through toll-like receptor 3." Molecular and cellular endocrinology 365.1 (2013): 75-83.
    Species: Human
    Sample type: Plasma
Easy to follow and works exactly as expected.

Biocogent, LLC
I have been using the IP-10 ELISA assay for several months to measure IP-10 levels in human plasma and purified from blood. It has very low background and works well for my projects. Controls produce consistent OD450 values when stored as directed and used within expirations listed.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.