Human IP-10 ELISA

$398.00
ELH-IP10-1

RayBio® Human IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
8 pg/ml

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Detection Range
8 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP- 2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10)
Accession Number
Gene Names
CXCL10, INP10, SCYB10
Gene ID
3627

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CXCL10

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample Type: Conditioned Media

  2. Gao Q., Camous X., Lu Y., et al. Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies. Neurobiol Aging. 2016 Mar;39:140-6. doi: 10.1016/j.neurobiolaging.2015.12.002.

  3. Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
    Species: Human
    Sample Type: Plasma

  4. Gunluoglu G., Seyhan E., Kazancioglu R., et al. Diagnosing latent tuberculosis in immunocompromised patients measuring blood IP-10 production capacity: an analysis of chronic renal failure patients. Intern Med. 2015;54(5):465-72. doi: 10.2169/internalmedicine.54.3245
    Species: Human
    Sample Type: Plasma

  5. Marozin S., Altonmonte J., Munoz-Alvarez K., et al. STAT3 inhibition reduces toxicity of oncolytic VSV and provides a potentially synergistic combination therapy for hepatocellular carcinoma. Cancer Gene Ther. 2015 Jun;22(6):317-25. doi: 10.1038/cgt.2015.23.
    Species: Human
    Sample Type: Conditioned Media

  6. Kine E., Trine B., Alexander H., et al. Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines. J Interferon Cytokine Res. 2015 Oct;35(10):759-70. doi: 10.1089/jir.2014.0171
    Species: Human
    Sample Type: Serum

  7. Kine E., Trine B., Alexander H., et al. Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines. J Interferon Cytokine Res. 2015 Oct;35(10):759-70. doi: 10.1089/jir.2014.0171
    Species: Human
    Sample Type: Plasma

  8. Spaks A., Jaunalksne I., Spaka I., et al. Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer. Anticancer Res. 2015 Dec;35(12):6979-83.

  9. Hardy GAD, Sieg S, Rodriguez B, et al. Interferon-α is the primary plasma type-I IFN in HIV-1 infection and correlates with immune activation and disease markers. PLoS ONE. 2013;8(2):e56527.
    Species: Human
    Sample Type: Plasma

  10. Wu C, Lu X, Wang X, et al. Clinical symptoms, immune factors, and molecular characteristics of an adult male in Shenzhen, China infected with influenza virus H5N1. J Med Virol. 2013 May;85(5):760-768.
    Species: Human
    Sample Type: Serum

  11. Fujiwara S., Nagai H., Oniki S., et al. Interleukin (IL)-17 versus IL-27: opposite effects on tumor necrosis factor-α-mediated chemokine production in human keratinocytes. Exp Dermatol. 2012 Jan;21(1):70-2. doi: 10.1111/j.1600-0625.2011.01384.x
    Species: Human
    Sample Type: Conditioned Media

  12. Abdel Fattah H., Sadek Sabbour G., Hamed A., et al. INTERFERON-GAMMA INDUCIBLE PROTEIN 10 (CXCL 10) AS A PREDICTOR OF LIVER FIBROSIS IN CHRONIC LIVER DISEASE. AAMJ, Vol. 10, N. 3, Sep, 2012, Suppl.
    Species: Human
    Sample Type: Serum

  13. Vargas-Inchaustegui D., Hogg A., Tulliano G., et al.CXCL10 Production by Human Monocytes in Response to Leishmania braziliensis Infection. Infect. Immun. January 2010 vol. 78 no. 1 301-308
    Species: Human
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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