Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
A4GALT, ABCB9, ABCC2, ABCC3, ABCD1, ABHD14B, Abl interactor 2, ABLIM1, ACADL, ACIN1, ACOT13, ACOT2, ACOX1, ACSBG1, ACSL1, ACSL5, ACSS3, ACYP2, ADCYAP1R1, ADK, ADSL, AIM2, AKR1A1, AKR1B10, Aladin, ALOX15B, ALOX5AP, AMFR, ANAPC13, Anoctamin-1, AP1B1, Aquaporin-1, Aquaporin-9, ARD1A, ARF3, ARID1A, ARID1B, ARL2BP, Aromatase, ASB2, ASF1A, ATIC, ATL3, ATOX1, ATP1B1, ATP6V0A2, ATP6V1F, ATPIF1, AZI2, B3GAT3, B3GNT3, BABAM2, BCL2L1, BCL2L12, BCL6, BDKRB1, BET1-like protein, BHLHE40, BLNK, BMF, BOLA1, BPHL, BTN3A3, BVES, CA7, Calmodulin-2, Calsequestrin-2, CAMK1, CAMK4, CAPZA2, CAPZB, Caspase-5, Caspase-6, Cathepsin K, CBFB, CBLC, CBR3, CD247, CD3E, CD81, CD8B, CDC37, CDC42, CDC42BPB, CDC6, CDCA3, CDH16, CDK6, CDS2, CEBPB, CGREF1, CHMP4A, CHN1, CHRNA7, CKMT1A, Claudin-7, CLCA1, CLPB, CMBL, CNOT6, CNPY2, COASY, COL6A3, COMMD8, CPLX3, CPSF6, CPT2, CPVL, CRABP1, CRABP2, CRADD, CRELD1, CRYZ, CSNK1G2, CSNK2A2, CST7, CUTC, DAO, DCLK1, DCUN1D1, DDX41, DHRS2, DHRS4, DHRS9, DHX15, DIS3L, DNA helicase MCM8, DNA ligase 1, DNAJC30, DNASE2, DNTT, DPF3, DPYSL5, DUSP14, Dynamin-1, DZIP1, E2F5, EBPL, ECD, ECH1, ECSIT, EEF1B2, EFNA1, EHD2, EIF1AY, EIF3D, EIF3G, EIF3J, EIF3K, EIF4EBP1, EIF5A2, Elastin, EMR1, Epsin-3, ERCC5, EWSR1, EXOSC10, F8, FAR2, FAU, FBL, FBXO6, FGFBP3, FHIT, FSTL5, GABARAPL2, GADD45G, Galactokinase, GALE, GALNS, GBP2, GCDH, GCM2, GFER, GGA1, GGA3, GLIPR1, GLT8D2, GMEB1, GMFG, GNG13, GNGT1, GOPC, GPD2, GPR37, GRIK2, GSTK1, GSTM5, GSTZ1, GUCA1A, HAT1, HDAC1, HDAC3, HDAC6, HERC2, HERPUD1, HIST1H2BB, HIST1H3A, HIST2H2BE, HNF1A, HNMT, HRAS, HSD3B1, HSPA6, HTR3B, HTRA3, IDI2, IDO2, IFITM2, IFN alpha 5, IFNA2, IFNW1, IGJ, IGSF8 (CD316), IMP3 , IMPA1, INSL5, Integrin alpha-E, Interferon alpha-10, Interferon alpha-8, Isthmin-1, ITCH, KCNJ12, KCNQ5, KIAA0101, KIAA1279, KLF13, KLF7, KLRC1, KRT24, KRT7, KRT78, KRT79, LAPTM4A, LIN52, LRIG1, LRP10, LRP11, LSM3, LSM7, LSP1, LTC4S, MAG, MAGOH, Major vault protein, MAN1A2, MAP4K3, MAPK1, MAPK8, MAPKAPK3, MAPRE3, MASP2, MAX, MCTS1, MECP2, MRPL11, MRPL12, MRPL15, MRPL30, MRPL44, MRPS2, MRPS5, MRTO4, MS4A1, MUS81, NAALADL1, Nanos homolog 1, NARS, NCKAP1, NDRG1, Netrin receptor UNC5A, NFE2L3, NGLY1, NMRAL1, NOS1AP, NPC1, NSFL1C, NTNG1, NTS, Nuclear pore glycoprotein p62 (Nucleoporin Nup62), Nucleolin, NUDC, NUDT1, NUP107, NUP50, OLFM4, OTX1, OXCT1, P2X purinoceptor 5, P2Y purinoceptor 6, PABPC3, PADI4, PAPOLA, PARD6B, PARVA, PCF11, PDCD10, PDCD6IP, PDE1C, PDE9A, PDK1, PDRG1, Pepsin A-4, PEX13, PEX19, PEX3, PFDN5, PFKFB4, PFKP, PFN2, PGS1, PHF1, PHF13, PIAS1, PIGK, PIK3CA, PIM1, PIPOX, PLBD2, PLK4, PLN, PLSCR3, PMCH, PML, PMM2, PNPT1, POLR3B, POP4, PPIE, PPIF, PPIP5K2, PPP1CC, PPP1R17, PPP2R5E, PPT1, PQBP1, PRAC1, PRDM2, PRICKLE2, PRKAR1A, PRKD2, PRKG2, PRMT5, PRMT6, Proenkephalin-B, Prohibitin-2, Protein BTG3, Protein FAM98A, Protein KIBRA, Protein MTSS 1, Protein orai-3, Protein OS-9, PRPF18, PRPF19, PSME1, PSME2, PSME3, PSMF1, PTGES, PTPN12, PXMP4, RAB11B, RAB27B, RAB31, RAB6A, RAB8B, RAD54B, RAET1L, RBKS, RBL2, RBM34, RBP5, RBP7, RELA, REXO1, RFK, RHOT1, Ribonuclease H1, RING1, RIOK2, RNASEH2B, RNF41, RNMT, RPL36, RPP14, RPRD1B, RRAGC, RUVBL1, RXFP3, SACM1L, SCARB1, SCLY, SCN2B, SCPEP1, SDF2, SDHB, SEC11C, SEC23B, SECTM1, SELM, SETD8, SF3B4, SHOC2, SIPA1, SIRT4, SLC19A3, SLC22A7, SLC22A8, SLC26A6, SLC27A4, SLC2A10, SLC34A1, SLC34A3, SLC39A8, SLC6A1, SLC9A3R1, SLK, SMG1, SMN1, SMPD2, SMYD2, SNRPD2, Sorting nexin-2, SP1, Sp-100, SP4, SPCS2, Speedy protein C, SPEG, Splicing factor 45, SRP72, SRSF10, SSR1, ST7, STAG1, STC-2, STK16, Syntaxin-8, SYT6, TAZ (WWTR1), TBCB, TBP, TFDP1, TJP1, TJP2, TLE3, TLK1, TP53I3, TPPP3, TPT1, TRH, tRNA, TSG101, TSPAN1, TST, TTF1, TTF2, TUBG1, TXNL4A, TXNRD1, TYMS, TYRP1, UBASH3A, UBE2F, UBE2H, UBE2I, UBE2M, UBE2T, UBE2W, UBE3A, Ubiquilin-2, UBXN6, UGDH, UGP2, UNC13C, UNG, uPA, UPF3B, UROS, USP30, USP46, UTP18, UXS1, VARS, VKORC1, VLDLR, VNN2, VPS25, VPS26A, VRK1, VSNL1, VTA1, VTI1A, VTI1B, WDR61, XCL2, XPC, XRCC1, XRCC2, ZFP36L2
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Membranes
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Detection Buffer C
- Detection Buffer D
- Wash Buffer 1
- Wash Buffer 2
- HRP-Conjugated Streptavidin Concentrate
- Plastic Sheets
- Spin Columns
- Labeling Buffer
- Lysis Buffer
- Plastic Incubation Tray
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- 2?5 ml tube
- 15 ml conical collection tube
- Tissue Paper, blotting paper or chromatography paper
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Block array membranes
- Incubate with Samples
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.