Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
A4GNT, AAK1, Abl interactor 1, ACKR1, ADAM33, ADAMTS3, ADAMTS8, ADGRA2, ADGRE3, ADGRG3, ADIPOR1, ADNP, ADORA2A, ADRB1, ADRBK2, AFAP1, AGR2, AGTR1, AICDA, AIRE, Alpha-sarcoglycan, Annexin A10, Annexin A13, APBA3, APH1A, APPL1, ARAF, ARHGEF12, ASC (PYCARD), ASCL2, ATAD2, ATF1, ATF4, ATG5, Atrophin-1, Aurora kinase B, Axin-2, BACH1, BCR, BRCA1, BRIX1, BTN3A2, BTRC, C12orf5, C1QTNF3, C3AR1, C5AR1, CARD9, Caveolin-3, CCND3, CCNE2, CCR10, CCRL1, CD1B, CD1C, CD1D, CD1E, CDK8, CDKN2AIP, CDKN2B, CDKN2C, CDX2, CEBPE, CELSR2, Cerebellin-4, CFTR, CHD1L, CHRM3, CHRM5, CHUK, CIDEC, Claudin-1, Claudin-10, Claudin-12, Claudin-15, Claudin-17, Claudin-19, Claudin-6, CLTCL1, CNMD, COMMD1, COPS5, Cornulin, COX4I1, COX4I2, CRTC2, CRTC3, CRX, CSRP2, CTBP1, CTCF, CUX1, CX3CR1, Cyclin A, Cytohesin-1, Dachshund homolog 2, Dapper homolog 3, DAXX, DDB2, DDIT3, DDX17, DDX5, DDX58, DECR1, Delta-sarcoglycan, DGKB, DGKD, DGKI, DGKZ, DISP2, DMPK, DNA, DNAH17, DOCK2, DOCK3, Docking protein 3, DPPA2, DRD1, DRD2, DUSP1, DVL1, DVL3, E2F1, E2F2, EBF1, EBF2, EBF3, EGFL7, EGLN1, EGLN2, EGLN3, EIF2AK3, EIF2AK4, EIF4B, EIF4G1, ENAH, Endomucin, ENPP3, EPAS1, EPS8, ESRRG, ETV1, ETV5, ETV6, EZH2, F2RL1, FAM123B, FERMT2, FLI1, FOS, FOXC1, FOXD3, FOXF2, FOXJ3, FOXK1, FOXL2, FOXP2, FOXP4, FRAT2, FXYD5, GAB2, GABBR1, GAK, Galectin-12, GALNT4, GAPDHS, GBX2, GCHFR, GFI1, GJA1, GLI1, GLIS1, Glycoprotein Xg, GPR101, GPR12, GPR183, GPR22, GPR26, GPR34, GPR37L1, GPRC5A, GPRC5B, Granzyme K, GSK3A, GUCY2C, HAX1, HES1, HHEX, HIF1AN, Histone H3.1t, HMGA1, HMGA2, HNF1B, HNF4G, HOXB13, HR, HS6ST2, HSD11B2, HSF2, HSF4, ICA1, ID2, IFITM3, IFNGR2, IGDCC3, IKK-B, IKZF1, IKZF3, IL17RE, IL4I1, Integrin alpha-9, Integrin beta-8, Intelectin-2, Interferon alpha-17, Interferon alpha-6, IRAK1, IRAK2, IRF2BP1, ITGA8, ITGB1BP1, ITM2A, ITM2C, KDM4A, KDM4C, KDM5B, KHDRBS1, KIAA1303, KLF12, KLF2, KLF5, KLF6, KPNA1, KPNA4, LEF1, LGI2, LHX1, Lipase member I, Lipoprotein lipase, LMO4, LOXL3, LPAR2, LPAR4, LPAR5, LPP, LRMP, LRP1B, LRP5, LRRK2, MAD1L1, MAF, MAFF, MAFG, MAFK, MAOA, MAP2, MAP2K3, MAP2K4, MAP3K10, MAP3K11, MAP3K14, MAP3K3, MAP3K7IP1, MAP4K5, MAPK11, MAPK7, MATN1, Matrilin-4, Max-like protein X, MBD3, MDC1, MEGF9, MELK, Menin, MGMT, Microcephalin, MITF, Mitofusin-1, MLKL, MRC2, MSH2, MSX2, MTF2, Mucin-19, MX1, MXI1, Myocardin, MYOD1, Nanos homolog 2, NCOA3, NETO2, NEUROD2, NFAM1, NFATC1, NKX2-1, NKX3-1, NME2, NNMT, NOD1, Nodal modulator 1, NR1D2, NR1I3, NR2C1, NR2C2, NR2E1, NR2E3, NR2F6, NR4A3, NUB1, Nucleoredoxin, OAS2, Occludin, ONECUT1, OPA1, Otoraplin, PADI2, PAK1, PAK3, Palladin, Pannexin-2, Parafibromin, PARD3, PAWR, PAX5, PAX6, PCDHA1, PDZK1, PEA15, Perforin-1, Phospholipase D1, Phospholipase D2, PHOX2B, PIBF1, PIK3CB, PIK3CD, PIK3R2, PIK3R4, PIK3R5, PINK1, Piwi-like protein 4, PLA2G16, PLCB1, PLCB3, PLCD3, PLCG2, PLEKHA1, PLSCR1, Polycystin-1, POU3F2, PPARA, PPARD, PPARGC1A, PPM2C, PPP1R1B, PRDM16, PREX1, PRKAA2, PRKAB2, PROKR1, Prominin-2, Protein HEXIM1, Protein THEMIS, Protein Wnt-10a, Protein Wnt-6, Protocadherin-19, PRSS22, PSENEN, PSMD4, PTCH1, PTGER1, PTGER2, PTGER3, PTK6, PTPN14, PTPRN, PTPRT, Pygopus homolog 1, RACGAP1, RAP2B, RARA, RARB, RARG, RARRES1, RB1, RBPJ, RELB, RHBDF2, RHEB, RIPK2, RIPK3, RIT1, RIT2, RND3, RNF14, RNF8, RORA, RPS6KA6, RUNX1, RXRA, RXRB, RXRG, Sacsin, Sal-like protein 1, Sal-like protein 4, SAMHD1, SATB1, SCAMP3, SCGB3A2, SHB, SIGMAR1, SIN3A, SLC12A2, SLC16A1, SLC17A7, SLC18A2, SLC1A3, SLC22A1, SLC22A2, SLC27A1, SLC27A2, SLC27A5, SLC6A3, SLC7A5, SLC8A1, SMAGP, SMARCA5, Smoothened homolog, SNAPIN, SOCS4, SOS2, SOX18, SOX5, SOX6, SP3, SP7, SPIB, SPRED2, SPRY2, SPRYD4, SPTBN2, SRPK2, Stathmin-2, STXBP2, STXBP3, SULF2, Supervillin, SUSD2, SVEP1, SWAP70, SYN1, Synaptophysin, Syntabulin, TAP2, TBK1, TBX5, TCF7, TCL1A, TCL1B, TDRD1, Tensin-4, Tetraspanin-2, Tetraspanin-9, TFR2, TGFB1I1, TIAM1, TICAM2, TINAG, TLE1, TLR5, TM4SF1, TMEM59, TMPRSS9, TNKS, TRAF1, TRAF5, TRAF6, TRIM28, TRIM5, TSPO, Tuberin, TWIST2, UCP1, UCP2, VISA, VSIG10L, VWA1, WASF1, WASF3, WDR5, XBP1, ZBTB17, ZBTB7A, ZEB1, ZEB2, ZFP90, ZG16B, ZMIZ1, ZNF366, ZNF71, ZSCAN10, ZSCAN21
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Membranes
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Detection Buffer C
- Detection Buffer D
- Wash Buffer 1
- Wash Buffer 2
- HRP-Conjugated Streptavidin Concentrate
- Plastic Sheets
- Spin Columns
- Labeling Buffer
- Lysis Buffer
- Plastic Incubation Tray
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- 2?5 ml tube
- 15 ml conical collection tube
- Tissue Paper, blotting paper or chromatography paper
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Block array membranes
- Incubate with Samples
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.