Human Leptin ELISA

$398.00
ELH-Leptin-1

RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
2 pg/ml

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Detection Range
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma)
20 - 50 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Leptin (Obese protein) (Obesity factor)
Accession Number
Gene Names
LEP, OB, OBS
Gene ID
3952

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • LEP

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Joseph D. Kumar S. Identifying clues to molecular etiology of multiple sclerosis in South Indian patients. Mult Scler Relat Disord. 2016 Jan;5:7-11. doi: 10.1016/j.msard.2015.10.002.
    Species: Human
    Sample Type: Serum

  2. Pektas M., Kurt A., Un I., et al. Effects of 17b-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase. Cytokine. 2015 Apr;72(2):130-4. doi: 10.1016/j.cyto.2014.12.020
    Species: Human
    Sample Type: Conditioned Media

  3. Youseef M. The Effect of Dietary Intervention by Low Carbohydrate Diet, and Low Fat Diet, on Weight Loss, Leptin and Adiponectin. Life Science Journal 2015;12(4)
    Species: Human
    Sample Type: Serum

  4. Kumar P., Srivastava N., Tiwari S., et al. Correlation of gender and age with serum adipokines level in north indian adults. Asian Journal of Medical Sciences | Sep-Oct 2015 | Vol 6 | Issue 5, 9-11
    Species: Human
    Sample Type: Serum

  5. Nabil N., El Sayyad M., et al. moderate exercise training has anorexogenic effect associated with improved oxidative stress in obese women. International Journal of Nutrition and Metabolism. Vol. & (4), pp 52-61, April 2015, DOI: 10.5897/IJNAM2015.0180
    Species: Human
    Sample Type: Plasma

  6. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample Type: Serum

  7. Dede N., Ipekci S., Kebapcilar L., et al. Influence of Exercise on Leptin, Adiponectin and Quality of Life in Type 2 Diabetics. Turk J Endocrinol Metab 2015; 19: 7-13
    Species: Human
    Sample Type: Serum

  8. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample Type: Serum

  9. Bhaktha G., et al. SERUM TNF-α AND LEPTIN LEVELS IN GRADED OBESE DIABETIC WOMEN- FREE FROM MICRO AND MACRO VASCULAR COMPLICATIONS. IJPCBS 2014, 4(3), 648-653
    Species: Human
    Sample Type: Serum

  10. Bil-lula I., et al. Infectobesity in the Polish Population - Evaluation of an Association between Adenoviruses Type 5, 31, 36 and Human Obesity. International Journal of Virology and Molecular Biology 2014, 3(1): 1-8. DOI: 10.5923/j.ijvmb.20140301.01
    Species: Human
    Sample Type: Plasma

  11. Bil-Lula I., et al. Is There any Link Between Visceral Obesity and Adenovirus Infections in the Polish Population? Adv Clin Exp Med 2014, 23, 3, 415-422
    Species: Human
    Sample Type: Plasma

  12. Das P., Bandyopadhyay SK., Biswas S., et al. CORRELATION OF OBESITY AND THE NOVEL ADIPOCYTOKINES, LEPTIN AND ADIPONECTIN WITH INSULIN RESISTANCE IN TYPE 2 DIABETES MELLITUS. Bangladesh J Physiol Pharmacol 2014;30(1):29-32.)
    Species: Human
    Sample Type: Serum

  13. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
    Species: Human
    Sample Type: Plasma

  14. Ha AW., et al. Plasma adipocytokines and antioxidants-status in Korean overweight and obese females with dyslipidemia. Nutr Res Pract. 2014 Aug;8(4):417-424. http://dx.doi.org/10.4162/nrp.2014.8.4.417
    Species: Human
    Sample Type: Plasma

  15. Jorgensen A., Nellemann C., Wohlfahr-Veje C. Interaction between paraoxonase 1 polymorphism and prenatal pesticide exposure on metabolic markers in children using a multiplex approach. Reprod Toxicol. 2014 Nov 22;51C:22-30. doi: 10.1016/j.reprotox.2014.11.005.
    Species: Human
    Sample Type: Serum

  16. Leisegang K., Bouic P., Menkveld R., Henkel R. Reproductive Biology and Endocrinology 2014, 12:34 http://www.rbej.com/content/12/1/34
    Species: Human
    Sample Type: Seminal Fluid

  17. Leisegang K., Bouic P., Menkveld R., Henkel R. Reproductive Biology and Endocrinology 2014, 12:34 http://www.rbej.com/content/12/1/34
    Species: Human
    Sample Type: Serum

  18. Lin Y., et al. Dietary fiber intake and its association with indicators of adiposity and serum biomarkers in European adolescents: the HELENA study. European Journal of Nutrition August 2014, Epub Ahead of Print. DOI 10.1007/s00394-014-0756-2
    Species: Human
    Sample Type: Serum

  19. Rathore S., Bidwai A. Association of Insulin Resistance and Selected Adipokines in Cardiovascular Disease with and without Metabolic Syndrome. JMSCR Volume 2, Issue 5, Pages 1176-1186, May 2014
    Species: Human
    Sample Type: Serum

  20. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013;8(3):e58541.
    Species: Human
    Sample Type: Conditioned Media

  21. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013;8(3):e58541.
    Species: Human
    Sample Type: Serum

  22. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013;8(3):e58541. doi:10.1371/journal.pone.0058541
    Species: Human
    Sample Type: Conditioned Media

  23. Dubois V, Delort L, Billard H, et al. Breast cancer and obesity: in vitro interferences between adipokines and proangiogenic features and/or antitumor therapies? PLoS ONE. 2013;8(3):e58541. doi:10.1371/journal.pone.0058541
    Species: Human
    Sample Type: Serum

  24. Hoo SP, Loh QL, Yue Z, et al. Preparation of a soft and interconnected macroporous hydroxypropyl cellulose methacrylate scaffold for adipose tissue engineering. J Mater Chem B. 2013 May 20; [Epub ahead of print] doi:10.1039/C3TB00446E
    Species: Human
    Sample Type: Conditioned Media

  25. Thanakun S., Watanabe H., Thaweboon S., Izumi Y. An effective technique for the processing of saliva for the analysis of leptin and adiponectin. Peptides. 2013 Sep;47:60-5. doi: 10.1016/j.peptides.2013.06.010
    Species: Human
    Sample Type: Saliva

  26. Volberg V., Harley KG., Aguilar RS., et al. Associations between perinatal factors and adiponectin and leptin in 9-year-old Mexican-American children. Pediatr Obes. 2013 Dec;8(6):454-63. doi: 10.1111/j.2047-6310.2012.00127.x.
    Species: Human
    Sample Type: Plasma

  27. Jimenez-Pavon D., Ortega F., Artero E., et al. Physical activity, fitness, and serum leptin concentrations in adolescents. J Pediatr. 2012 Apr;160(4):598-603.e2. doi: 10.1016/j.jpeds.2011.09.058.
    Species: Human
    Sample Type: Serum

  28. Fahlbusch F., Ruebner M., Volkert G
    Species: Human
    Sample Type: Conditioned Media

  29. Schneiderman J., Schaefer K., Kolodgie F., et al. Leptin locally synthesized in carotid atherosclerotic plaques could be associated with lesion instability and cerebral emboli. J Am Heart Assoc. 2012 Oct;1(5):e001727. doi: 10.1161/JAHA.112.001727.
    Species: Human
    Sample Type: Conditioned Media

  30. Badler M., Reitmayer C., Arndt C., et al. Age dependency of estrogen responsiveness in the uterus and adipose tissue of aromatase-knockout (ArKO) mice. J Steroid Biochem Mol Biol. 2012 Jan;128(1-2):29-37. doi: 10.1016/j.jsbmb.2011.09.009
    Species: Mouse
    Sample Type: Plasma

  31. Andersen HR, Wohlfahrt-Veje C, Dalga°rd C, Christiansen L, Main KM, et al. (2012) Paraoxonase 1 Polymorphism and Prenatal Pesticide Exposure Associated with Adverse Cardiovascular Risk Profiles at School Age. PLoS ONE 7(5): e36830. doi:10.1371/journal.pone.0036830
    Species: Human
    Sample Type: Serum

  32. Brydon L. Adiposity, leptin and stress reactivity in humans. Biological Psychology. 2011;86(2):114-120. doi:10.1016/j.biopsycho.2010.02.010.
    Species: Human
    Sample Type: Plasma

  33. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample Type: Serum

  34. Labayen, I., et al. "Association between the FTO rs9939609 polymorphism and leptin in European adolescents: a possible link with energy balance control. The HELENA study." International Journal of Obesity 35.1 (2011): 66-71.

  35. Tzschoppe, Anja, et al. "Dexamethasone stimulates the expression of leptin and 11β-HSD2 in primary human placental trophoblastic cells." European Journal of Obstetrics & Gynecology and Reproductive Biology 156.1 (2011): 50-55.

  36. Kerem M, Ferahkose Z, Yilmaz U, Pasaoglu H, Ofluoglu E, et al. Adipokines and ghrelin in gastric cancer cachexia.World J Gastroenterol. 2008;14(23):3633-3641.
    Species: Human
    Sample Type: Serum

  37. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-α on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006;452:418-427
    Species: Human
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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