Human MCP-1 ELISA

RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-MCP1-1
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Antigen Information

Accession Number:
  • P13500
Gene ID:
  • 6347
Gene Symbols:
  • CCL2
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 500 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
    Species: Human
    Sample type: Plasma
  2. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample type: Serum
  3. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample type: Conditioned Media
  4. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF?7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016
    Species: Human
    Sample type: Conditioned Media
  5. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample type: Conditioned Media
  6. Filkova M., Vernerova Z., Hulejova H., et al. Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis. Cytokine. 2015 May;73(1):36-43. doi: 10.1016/j.cyto.2015.01.019.
    Species: Human
    Sample type: Conditioned Media
  7. Bakry R., Sayed D., Sayed S., et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia. Comp Clin Pathol (2015) 24:1573-1577, DOI 10.1007/s00580-015-2118-8
    Species: Human
    Sample type: Serum
  8. Zhang H., Zhao C., Wang S., et al. Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells. J Transl Med. 2015 Jun 4;13:178. doi: 10.1186/s12967-015-0536-7.
    Species: Human
    Sample type: Conditioned Media
  9. Andia I., Rubio-Azpeitia E., Maffulli N. Platelet-rich plasma modulates the secretion of inflammatory/angiogenic proteins by inflamed tenocytes. Clin Orthop Relat Res. 2015 May;473(5):1624-34. doi: 10.1007/s11999-015-4179-z.
    Species: Human
    Sample type: Conditioned Media
  10. Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
    Species: Human
    Sample type: Plasma
Easy and precise

University of Houston
Easy to follow!

Oregon Health & Science University
Very easy instructions to follow and the kit worked very well.

University of Houston
Easy to follow and works exactly as expected.

Biocogent, LLC
Used for analysis of human serum samples, easy to use, clean definitive results for varied human samples.

Washington University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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