Human MCP-1 ELISA

$398.00
ELH-MCP1-1

RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
2 pg/ml

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Detection Range
2 pg/ml - 500 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
Accession Number
Gene Names
CCL2, MCP1, SCYA2
Gene ID
6347

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CCL2

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample Type: Serum

  2. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample Type: Conditioned Media

  3. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF‑7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016

  4. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample Type: Conditioned Media

  5. Filkova M., Vernerova Z., Hulejova H., et al. Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis. Cytokine. 2015 May;73(1):36-43. doi: 10.1016/j.cyto.2015.01.019.
    Species: Human
    Sample Type: Conditioned Media

  6. Bakry R., Sayed D., Sayed S., et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia. Comp Clin Pathol (2015) 24:1573-1577, DOI 10.1007/s00580-015-2118-8
    Species: Human
    Sample Type: Serum

  7. Zhang H., Zhao C., Wang S., et al. Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells. J Transl Med. 2015 Jun 4;13:178. doi: 10.1186/s12967-015-0536-7.
    Species: Human
    Sample Type: Conditioned Media

  8. Andia I., Rubio-Azpeitia E., Maffulli N. Platelet-rich plasma modulates the secretion of inflammatory/angiogenic proteins by inflamed tenocytes. Clin Orthop Relat Res. 2015 May;473(5):1624-34. doi: 10.1007/s11999-015-4179-z.
    Species: Human
    Sample Type: Conditioned Media

  9. Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
    Species: Human
    Sample Type: Plasma

  10. Talacua H., Smits A., Muylaert D., et al. In Situ Tissue Engineering of Functional Small-Diameter Blood Vessels by Host Circulating Cells Only. Tissue Eng Part A. 2015 Oct;21(19-20):2583-94. doi: 10.1089/ten.TEA.2015.0066.
    Species: Human
    Sample Type: Artificial Scaffolds

  11. Huggins C., Pearce S., Peri F., et al. A novel small molecule TLR4 antagonist (IAXO-102) negatively regulates non-hematopoietic toll like receptor 4 signalling and inhibits aortic aneurysms development. Atherosclerosis. 2015 Oct;242(2):563-70. doi: 10.1016/j.atherosclerosis.2015.08.010
    Species: Human
    Sample Type: Cell Lysate

  12. Caspar-Bauguil S., Kolditz C., Lefort C., et al. Fatty acids from fat cell lipolysis do not activate an inflammatory response but are stored as triacylglycerols in adipose tissue macrophages. Diabetologia. 2015 Nov;58(11):2627-36. doi: 10.1007/s00125-015-3719-0.
    Species: Human
    Sample Type: Conditioned Media

  13. Bauer D., Redmon N., Mazzio E., et al. Diallyl disulfide inhibits TNFα induced CCL2 release through MAPK/ERK and NF-Kappa-B signaling. Cytokine. 2015 Sep;75(1):117-26. doi: 10.1016/j.cyto.2014.12.007.
    Species: Human
    Sample Type: Conditioned Media

  14. Bakry, R., Sayed, D., Sayed, S. et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia, Comp Clin Pathol (2015) 24: 1573. doi:10.1007/s00580-015-2118-8
    Species: Human
    Sample Type: Serum

  15. Cieslik P., Hrycek A. Pentraxin 3 as a biomarker of local inflammatory response to vascular injury in systemic lupus erythematosus. Autoimmunity
    Species: Human
    Sample Type: Serum

  16. Lv M., et al. miR141-CXCL1-CXCR2 Signaling-Induced Treg Recruitment Regulates Metastases and Survival of Non-Small Cell Lung Cancer. Molecular Cancer Therapeutics. OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448
    Species: Human
    Sample Type: Conditioned Media

  17. Gupta M, Chaturvedi R, Jain A. Role of monocyte chemoattractant protein-1 (MCP-1) as an immune-diagnostic biomarker in the pathogenesis of chronic periodontal disease. Cytokine. 2013 Mar;61(3):892-897.
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  18. Gupta M, Chaturvedi R, Jain A. Role of monocyte chemoattractant protein-1 (MCP-1) as an immune-diagnostic biomarker in the pathogenesis of chronic periodontal disease. Cytokine. 2013 Mar;61(3):892-897.
    Species: Human
    Sample Type: Saliva

  19. Gupta M, Chaturvedi R, Jain A. Role of monocyte chemoattractant protein-1 (MCP-1) as an immune-diagnostic biomarker in the pathogenesis of chronic periodontal disease. Cytokine. 2013 Mar;61(3):892-897.
    Species: Human
    Sample Type: Serum

  20. Leow K-Y, Goh WWB, Tan S-Z, et al. Variant screening of the serum amyloid A1 gene and functional study of the p.Gly90Asp variant for its role in atherosclerosis. Atherosclerosis. 2013 Mar;227(1):112-117.
    Species: Human
    Sample Type: Conditioned Media

  21. Lin Y., et al. A panel of four cytokines predicts the prognosis of patients with malignant gliomas. J Neurooncol (2013) 114:199-208, DOI 10.1007/s11060-013-1171-x
    Species: Human
    Sample Type: Plasma

  22. Rocher, Crystal, and Dinender K. Singla. "SMAD-PI3K-Akt-mTOR Pathway Mediates BMP-7 Polarization of Monocytes into M2 Macrophages." PLoS One. NCBI, 20 Dec. 2013. Web.
    Species: Human
    Sample Type: Conditioned Media

  23. Chen YL., Huang KF., Kuo WC., et al. Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins. Biochem. J. (2012) 442, 403-412. doi:10.1042/BJ20110535
    Species: Human
    Sample Type: Conditioned Media

  24. Ebud El-Aziz SA., et al. Platelet activating factor and monocyte chemoattractant protein-1 in children with refractory epilepsy. Egypt J Pediatr Allergy Immunol 2012;10(1):13-18.
    Species: Human
    Sample Type: Serum

  25. Filková M, Hulejová H, Kuncová K, et al. Resistin in idiopathic inflammatory myopathies. Arthritis Research & Therapy. 2012;14(3):R111. doi:10.1186/ar3836.
    Species: Human
    Sample Type: Serum

  26. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Human
    Sample Type: Conditioned Media

  27. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Human
    Sample Type: Tissue Lysate

  28. Rocher C., Singla R., Singal P., et al. Bone morphogenetic protein 7 polarizes THP-1 cells into M2 macrophages. Can J Physiol Pharmacol. 2012 Jul;90(7):947-51. doi: 10.1139/y2012-102.
    Species: Human
    Sample Type: Conditioned Media

  29. Leow K., Goh W., Heng C. Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages. Inflamm Res. 2012 Apr;61(4):391-8. doi: 10.1007/s00011-011-0424-4.
    Species: Human
    Sample Type: Conditioned Media

  30. Anand A, Sharma NK, Gupta A, Prabhakar S, Sharma SK, et al. (2012) Single Nucleotide Polymorphisms in MCP-1 and Its Receptor Are Associated with the Risk of Age Related Macular Degeneration. PLoS ONE 7(11): e49905. doi:10.1371/journal.pone.0049905
    Species: Human
    Sample Type: Serum

  31. Said, Neveen, et al. "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." The Journal of clinical investigation 121.1 (2011): 132-147.

  32. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-α on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006;452:418-427
    Species: Human
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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