Human MMP-1 ELISA Kit

RayBio® Human MMP-1 ELISA Kit for cell culture supernatants, heparin treated plasma, serum, and lysate samples. EDTA and Citrate are not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Sensitivity	8 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	8 pg/ml - 18000 pg/ml
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-2, - 3, -9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)	2 - 10 fold
Accession Number	P03956
Gene Id	4312
Gene Symbols	MMP1, CLG
Protein Name / Synonyms	Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase 27 kDa interstitial collagenase]
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Shipping Type	Blue ice
Storage	≤-20°C

Target Background

MMP-1 is a member of the large family of matrix metalloproteinase (MMP) that degrades substrates, such as gelatin, nidogen, collagen type 3, collagen type 4, laminin-1, proteoglycans, elastin, etc. Most MMP proteins are activated after being cleaved by extracellular proteinases. The matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological pathway: embryonic development, reproduction, tissue remodeling. MMP is also involved in disease processes, such as arthritis and metastasis.

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

ELH-MMP1 was used to measure MMP1 secretion in human gingival fibroblasts treated with cholesterol. Figure adapted from Tran et al., Experimental & Molecular Medicine, 2023.

ELH-MMP1-1 was used to measure MMP-1 levels in human gingival fibroblast culture media following lipopolysaccharide stimulation with or without S-1-propenyl-L-cysteine treatment. Figure adapted from Nango et al., PLOS ONE, 2023.

ELH-MMP1 was used to measure MMP-1 levels in culture supernatants from human hip chondrocytes (osteoarthritis vs non-osteoarthritis donors) under IL-1β or TNF stimulation. Figure adapted from Eitner et al., Biomedicines, 2022.

ELH-MMP1-1 was used to measure MMP-1 levels in fibroblast-like synoviocyte culture supernatants following Hedgehog pathway siRNA treatment. Figure adapted from Lin et al., Molecular Therapy – Nucleic Acids, 2023.

ELH-MMP1-1 was used to measure MMP1 levels in MG63 osteoblast-like cell culture supernatants following cold atmospheric plasma treatment. Figure adapted from Eggers et al., Odontology, 2020.

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Additional Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µL to 1 µL volumes
Adjustable 1-25 µL pipettes for reagent preparation
100 µL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µL of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (ELH-MMP1.pdf)

Datasheet (ELH-MMP1-CL.pdf)

SDS (ELH-MMP1.pdf)

SDS (ELH-MMP1-CL.pdf)

Excel (5af824478c7690acd2cf4518aeaae2058a44fa75.xlsm)

Target Background

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Additional Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µL to 1 µL volumes
Adjustable 1-25 µL pipettes for reagent preparation
100 µL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µL of Stop Solution to each well.
Read at 450 nm immediately.

Featured Products

Human MMP-1 ELISA Kit

Product Description

Specifications

Target Background

Product Features

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Human MMP-1 ELISA KitHuman MMP-1 ELISA Kit

Product Description

Specifications

Related Products

Target Background

Product Features

Standard Curves

Spiking & Recovery Results

Linearity Results

Typical Data

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Great product

Great product

Ask A Question

Great product

Great product

Related Products

NexaTag® Human MMP-1 qIPCR ELISA Kit

Human MMP Array Q1

Human Cytokine Array Q5

Human MMP Array C1

Human MMP-1 ELISA Kit

NexaTag® Human MMP-1 qIPCR ELISA Kit

Human MMP Array Q1

Human Cytokine Array Q5

Human MMP Array C1

Human MMP-1 ELISA Kit