Human MMP-13 ELISA

$398.00
ELH-MMP13-1

RayBio® Human MMP-13 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Product Features

Sensitivity
6 pg/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
6 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Species Detected
Human
Protein Name & Synonyms
Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13)
Accession Number
Gene Names
MMP13
Gene ID
4322

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • MMP13

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Chijimatsu R., Kunugiza Y., Taniyama Y., et al. Expression and pathological effects of periostin in human osteoarthritis cartilage. BMC Musculoskelet Disord. 2015; 16: 215. Published online 2015 Aug 21. doi: 10.1186/s12891-015-0682-3
    Species: Human
    Sample Type: Conditioned Media

  2. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
    Species: Human
    Sample Type: Conditioned Media

  3. Zhang X., Zhu Y., Chen X., Zhang Y., Zhang Y., et al. Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes. International Immunopharmacology, 2014 Epub Ahead of Print. http://dx.doi.org/10.1016/j.intimp.2014.05.006
    Species: Human
    Sample Type: Conditioned Media

  4. Balasubramanian SA, Pye DC, Willcox MDP. Effects of eye rubbing on the levels of protease, protease activity and cytokines in tears: relevance in keratoconus. Clin Exp Optom. 2013;96(2):214-218.
    Species: Human
    Sample Type: Tears

  5. Rhim E-M, Ahn S-J, Kim J-Y, et al. Stimulation of Matrix Metalloproteinases by Tumor Necrosis Factor-α in Human Pulp Cell Cultures. J Endodontics. 2013 Jun;39(6):795-800.
    Species: Human
    Sample Type: Conditioned Media

  6. Yuan W, Zhao M-D, Yuan F-L, et al. Association of endothelin-1 expression and cartilaginous endplate degeneration in humans. PLoS ONE. 2013;8(4):e60062.
    Species: Human
    Sample Type: Conditioned Media

  7. Toegel,S. et al. Caesalpinia sappan extract inhibits IL1β-mediated overexpression of matrix metalloproteinases in human chondrocytes.Genes Nutr. 2012 Apr;7(2):307-18. doi: 10.1007/s12263-011-0244-8. Epub 2011 Aug 18.
    Species: Human
    Sample Type: Conditioned Media

  8. Soydinc H., Sak M., Evliyaoglu O., et al. Maternal plasma prolidase, matrix metalloproteinases 1 and 13, and oxidative stress levels in pregnancies complicated by preterm premature rupture of the membranes and chorioamnionitis. J Turk Ger Gynecol Assoc. 2012 Sep 1;13(3):172-7. doi: 10.5152/jtgga.2012.23.
    Species: Human
    Sample Type: Plasma

  9. Jacob J., Eisemon E., Sheibani-Rad S., et al. Matrix metalloproteinase levels as a marker for rotator cuff tears. Orthopedics. 2012 Apr;35(4):e474-8. doi: 10.3928/01477447-20120327-18.
    Species: Human
    Sample Type: Tissue Lysate

Write a review

No posts found

U.S. International

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Cart content Cart content

Customers Also Bought Customers Also Bought