Human MMP-2 ELISA

$398.00
ELH-MMP2-1

RayBio® Human MMP-2 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Product Features

Sensitivity
3,500 pg/ml

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Detection Range
3.5 ng/ml - 800 ng/ml
Recommended Dilution (serum & plasma)
5 - 50 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 3, -7, -8, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) (TBE-1)
Accession Number
Gene Names
MMP2, CLG4A
Gene ID
4313

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • MMP2

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Agardan N., Degim Z., Yilmaz S., et al. The Effectiveness of Raloxifene-Loaded Liposomes and Cochleates in Breast Cancer Therapy. AAPS PharmSciTech. 2016 Aug;17(4):968-77. doi: 10.1208/s12249-015-0429-3.
    Species: Human
    Sample Type: Conditioned Media

  2. Wang X., Zhou Q., Lu Y., et al. Glycyrrhetinic acid potently suppresses breast cancer invasion and metastasis by impairing the p38 MAPK-AP1 signaling axis. Expert Opin Ther Targets. 2015 May;19(5):577-87. doi: 10.1517/14728222.2015.1012156.
    Species: Human
    Sample Type: Conditioned Media

  3. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample Type: BAL Fluid

  4. Wang C., Chang Q., Sun X., et al. Angiotensin II Induces an Increase in Matrix Metalloproteinase 2 Expression in Aortic Smooth Muscle Cells of Ascending Thoracic Aortic Aneurysms Through JNK, ERK1/2, and p38 MAPK Activation. J Cardiovasc Pharmacol. 2015 Sep;66(3):285-93. doi: 10.1097/FJC.0000000000000276.
    Species: Human
    Sample Type: Plasma

  5. Abdel-Latif M. Plasma Levels of Matrix Metalloproteinase (MMP)-2, MMP-9 and Tumor Necrosis Factor-α in Chronic Hepatitis C Virus Patients. Open Microbiol J. 2015 Aug 31;9:136-40. doi: 10.2174/1874285801509010136.
    Species: Human
    Sample Type: Plasma

  6. Kapelouzou A., Tsourelis L., Kaklamanis L., et al. Serum and tissue biomarkers in aortic stenosis. Glob Cardiol Sci Pract. 2015 Nov 13;2015(4):49. doi: 10.5339/gcsp.2015.49
    Species: Human
    Sample Type: Serum

  7. Domienik-Karłowicz J., et al. Emerging Markers of Atherosclerosis Before and After Bariatric Surgery. Obesity Surgery Sept 2014. epub ahead of print. DOI:10.1007/s11695-014-1407-7
    Species: Human
    Sample Type: Serum

  8. Gluba-Brzozka A., et al. Markers of increased cardiovascular risk in patients with chronic kidney disease. Lipids in Health and Disease 2014, 13:135 doi:10.1186/1476-511X-13-135
    Species: Human
    Sample Type: Serum

  9. Kılıç Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anadolu Kardiyol Derg 2014; 14: 531-41. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample Type: Serum

  10. Kilic Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anatolian J of Card. Published ahead of print, June 25th 2014. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample Type: Serum

  11. Slivka P., et al. Fractionation of an ECM hydrogel into structural and soluble components reveals distinctive roles in regulating macrophage behavior. Biomater. Sci., 2014, Advance Article DOI: 10.1039/C4BM00189C
    Species: Human
    Sample Type: Tissue Lysate

  12. Sun Y., Wang X., Zhou Q., et al. Inhibitory effect of emodin on migration, invasion and metastasis of human breast cancer MDA-κ-231 cells in vitro and in vivo. Oncology Reports Vol, 2015, 33, (1), 338-346. DOI: 10.3892/or.2014.3585
    Species: Human
    Sample Type: Conditioned Media

  13. Yang H, Wang B, Wang T, Xu L, He C, et al. (2014) Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via LipopolysaccharideStimulation and Is Overexpressed in Patients with Lymph Node Metastasis. PLoS ONE 9(10): e109980. doi:10.1371/journal.pone.0109980
    Species: Human
    Sample Type: Conditioned Media

  14. Hampel U, Klonisch T, Sel S, et al. Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface. Endocrinology. 2013;154(6):2034-2045.
    Species: Human
    Sample Type: Conditioned Media

  15. Wang X, Du J, Zhang T, et al. Inhibitory effects of PC-SPESII herbal extract on human breast cancer metastasis. Evid-Based Compl Alt Med. 2013;2013:894386. doi:10.1155/2013/894386
    Species: Human
    Sample Type: Conditioned Media

  16. Yücel C, Değim Z, Yilmaz S. Nanoparticle and liposome formulations of doxycycline: Transport properties through Caco-2 cell line and effects on matrix metalloproteinase secretion. Biomed. Pharmacother. 2013 Mar 23;
    Species: Human
    Sample Type: Conditioned Media

  17. Chim H., Armijo BS., Miller E., et al. Propranolol induces regression of hemangioma cells through HIF-1α-mediated inhibition of VEGF-A. Ann Surg. 2012 Jul;256(1):146-56. doi: 10.1097/SLA.0b013e318254ce7a.
    Species: Human
    Sample Type: Conditioned Media

  18. Toegel,S. et al. Caesalpinia sappan extract inhibits IL1β-mediated overexpression of matrix metalloproteinases in human chondrocytes.Genes Nutr. 2012 Apr;7(2):307-18. doi: 10.1007/s12263-011-0244-8. Epub 2011 Aug 18.
    Species: Human
    Sample Type: Conditioned Media

  19. Ortak H., Soguy E., Tas U., et al. The relation between keratoconus and plasma levels of MMP-2, zinc, and SOD. Cornea. 2012 Sep;31(9):1048-51. doi: 10.1097/ICO.0b013e318254c028.
    Species: Human
    Sample Type: Plasma

  20. Kaminska-Winciorek G., Deja G., Polanska J., Jarosz-Chobot P. The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study. Int J Clin Pract. 2012 Apr;66(4):374-7. doi: 10.1111/j.1742-1241.2011.02702.x.
    Species: Human
    Sample Type: Serum

  21. Zheng L., Huang Y., Song W., et al. Fluid shear stress regulates metalloproteinase-1 and 2 in human periodontal ligament cells: involvement of extracellular signal-regulated kinase (ERK) and P38 signaling pathways. J Biomech. 2012 Sep 21;45(14):2368-75. doi: 10.1016/j.jbiomech.2012.07.013
    Species: Human
    Sample Type: Purified Protein

  22. Leng L. Jiang T., Zhang Y. TLR9 expression is associated with prognosis in patients with glioblastoma multiforme. J Clin Neurosci. 2012 Jan;19(1):75-80. doi: 10.1016/j.jocn.2011.03.037.
    Species: Human
    Sample Type: Conditioned Media

  23. Prasadm I., Crawford R., Xiao Y. Aggravation of ADAMTS and matrix metalloproteinase production and role of ERK1/2 pathway in the interaction of osteoarthritic subchondral bone osteoblasts and articular cartilage chondrocytes -- possible pathogenic role in osteoarthritis. J Rheumatol. 2012 Mar;39(3):621-34. doi: 10.3899/jrheum.110777
    Species: Human
    Sample Type: Conditioned Media

  24. Gach, K., Szemraj, J., Wyrębska, A. et al. Mol Biol Rep (2011) 38: 1231. doi:10.1007/s11033-010-0222-z
    Species: Human
    Sample Type: Conditioned Media

  25. Yigit, Özgür, et al. "The effect of corticosteroid on metalloproteinase levels of nasal polyposis 1." The Laryngoscope 121.3 (2011): 667-673.

  26. Yigit, Özgür, et al. "The effect of corticosteroid on metalloproteinase levels of nasal polyposis 1." The Laryngoscope 121.3 (2011): 667-673.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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