Human MMP-2 ELISA

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RayBio® Human MMP-2 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

ELH-MMP2-1
+

Antigen Information

Accession Number:
  • P08253
Gene ID:
  • 4313
Gene Symbol:
  • CLG4A
  • MMP2
Protein Name & Synonyms:
72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) (TBE-1) [Cleaved into: PEX]
Target Species:
  • Human

Assay Format

Detection Range:
3.5 ng/ml - 800 ng/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
3,500 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 3, -7, -8, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Agardan N., Degim Z., Yilmaz S., et al. The Effectiveness of Raloxifene-Loaded Liposomes and Cochleates in Breast Cancer Therapy. AAPS PharmSciTech. 2016 Aug;17(4):968-77. doi: 10.1208/s12249-015-0429-3.
    Species: Human
    Sample type: Conditioned Media
  2. Wang X., Zhou Q., Lu Y., et al. Glycyrrhetinic acid potently suppresses breast cancer invasion and metastasis by impairing the p38 MAPK-AP1 signaling axis. Expert Opin Ther Targets. 2015 May;19(5):577-87. doi: 10.1517/14728222.2015.1012156.
    Species: Human
    Sample type: Conditioned Media
  3. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample type: BAL Fluid
  4. Wang C., Chang Q., Sun X., et al. Angiotensin II Induces an Increase in Matrix Metalloproteinase 2 Expression in Aortic Smooth Muscle Cells of Ascending Thoracic Aortic Aneurysms Through JNK, ERK1/2, and p38 MAPK Activation. J Cardiovasc Pharmacol. 2015 Sep;66(3):285-93. doi: 10.1097/FJC.0000000000000276.
    Species: Human
    Sample type: Plasma
  5. Abdel-Latif M. Plasma Levels of Matrix Metalloproteinase (MMP)-2, MMP-9 and Tumor Necrosis Factor-? in Chronic Hepatitis C Virus Patients. Open Microbiol J. 2015 Aug 31;9:136-40. doi: 10.2174/1874285801509010136.
    Species: Human
    Sample type: Plasma
  6. Kapelouzou A., Tsourelis L., Kaklamanis L., et al. Serum and tissue biomarkers in aortic stenosis. Glob Cardiol Sci Pract. 2015 Nov 13;2015(4):49. doi: 10.5339/gcsp.2015.49
    Species: Human
    Sample type: Serum
  7. Domienik-Kar?owicz J., et al. Emerging Markers of Atherosclerosis Before and After Bariatric Surgery. Obesity Surgery Sept 2014. epub ahead of print. DOI:10.1007/s11695-014-1407-7
    Species: Human
    Sample type: Serum
  8. Gluba-Brzozka A., et al. Markers of increased cardiovascular risk in patients with chronic kidney disease. Lipids in Health and Disease 2014, 13:135 doi:10.1186/1476-511X-13-135
    Species: Human
    Sample type: Serum
  9. K?l?ç Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anadolu Kardiyol Derg 2014; 14: 531-41. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample type: Serum
  10. Kilic Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anatolian J of Card. Published ahead of print, June 25th 2014. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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