Human MMP-9 ELISA

$398.00
ELH-MMP9-1

RayBio® Human MMP-9 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Product Features

Sensitivity
10 pg/ml

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Detection Range
10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma)
300 - 3,000 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Matrix metalloproteinase-9 (MMP-9) (EC 3.4.24.35) (92 kDa gelatinase) (92 kDa type IV collagenase) (Gelatinase B) (GELB)
Accession Number
Gene Names
MMP9, CLG4B
Gene ID
4318

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • MMP9

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample Type: Serum

  2. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample Type: Cell Lysate

  3. Wu L., Liu X., Xie Q. Osteopontin facilitates invasion in human trophoblastic cells via promoting matrix metalloproteinase-9 in vitro. Int J Clin Exp Pathol. 2015; 8(11): 14121-14130.
    Species: Human
    Sample Type: Conditioned Media

  4. Devanarayanan S., Nandeesha H., Kattimani S., Sarkar S. Relationship between matrix metalloproteinase-9 and oxidative stress in drug-free male schizophrenia: a case control study. Clin Chem Lab Med. 2016 Mar;54(3):447-52. doi: 10.1515/cclm-2015-0212.
    Species: Human
    Sample Type: Serum

  5. Prato M., Khadjavi A., Magnetto C., et al. Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta. Biosci Biotechnol Biochem. 2016;80(3):466-72. doi: 10.1080/09168451.2015.1095068
    Species: Human
    Sample Type: Conditioned Media

  6. Hanedan O., Baykan A., Sezer S., et al. Evaluation of Cardiovascular Changes in Children with BAVs. Pediatr Cardiol. 2016 Mar;37(3):472-81. doi: 10.1007/s00246-015-1302-6.

  7. Humbert P., Fanian F., Lihoreau T., et al. Mécano-Stimulation™ of the skin improves sagging score and induces beneficial functional modification of the fibroblasts: clinical, biological, and histological evaluations. Clin Interv Aging. 2015 Feb 2;10:387-403. doi: 10.2147/CIA.S69752
    Species: Human
    Sample Type: Conditioned Media

  8. Naegelen I., Plancon S., Nicot N., et al. An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells. J Leukoc Biol. 2015 Mar;97(3):557-71. doi: 10.1189/jlb.3A0514-254RR
    Species: Human
    Sample Type: Conditioned Media

  9. Nirala B., Gohil NK. EFFECT OF GLYCATED SERUM ALBUMIN ON FUNCTIONAL MARKERS IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS IN THE PRESENCE OF SHEAR STRESS. J. Mech. Med. Biol. DOI: 10.1142/S0219519415500268
    Species: Human
    Sample Type: Cell Lysate

  10. Gulino G., Magnetto C., Khadjavi A., et al. Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes. Mediators Inflamm. 2015;2015:964838. doi: 10.1155/2015/964838.
    Species: Human
    Sample Type: Conditioned Media

  11. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample Type: BAL Fluid

  12. Khadjavi A., Magnetto C., Panariti A., et al. Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing. Toxicol Appl Pharmacol. 2015 Aug 1;286(3):198-206. doi: 10.1016/j.taap.2015.04.015
    Species: Human
    Sample Type: Conditioned Media

  13. Markiewicz L., Pytel D., Mucha B., Szymanek K., et al. Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. BioMed Research International Volume 2015 (2015), Article ID 812503, 8 pages http://dx.doi.org/10.1155/2015/812503
    Species: Human
    Sample Type: Aqueous Humor

  14. Zhang Y., Lin P., Jiang H., et al. Extensive serum biomarker analysis in patients with ST segment elevation myocardial infarction (STEMI). Cytokine. 2015 Dec;76(2):356-62. doi: 10.1016/j.cyto.2015.06.015.
    Species: Human
    Sample Type: Serum

  15. Wadood S., Shawk R., Hashem R. Variants of MMP-9 and TIMP-1 Levels Could be a Predictor of an Early Development of Cardiovascular Diseases in Type 2 Diabetes among Iraqi Patients. Iraqi Journal of Science, 2015, Vol 56, No.1C, pp:622-632
    Species: Human
    Sample Type: Serum

  16. Wang W., Song X., Chen Y., et al. The Long-Term Influence of Tissue Inhibitor of Matrix Metalloproteinase-1 in Patients with Mild to Moderate Coronary Artery Lesions in a Chinese Population: A 7-Year Follow-Up Study. Cardiology. 2015;132(3):151-8. doi: 10.1159/000430885
    Species: Human
    Sample Type: Plasma

  17. Basilico N., Magnetto C., D'Alessandro D., et al. Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium. Toxicol Appl Pharmacol. 2015 Nov 1;288(3):330-8. doi: 10.1016/j.taap.2015.08.005.
    Species: Human
    Sample Type: Conditioned Media

  18. Abdel-Latif M. Plasma Levels of Matrix Metalloproteinase (MMP)-2, MMP-9 and Tumor Necrosis Factor-α in Chronic Hepatitis C Virus Patients. Open Microbiol J. 2015 Aug 31;9:136-40. doi: 10.2174/1874285801509010136.
    Species: Human
    Sample Type: Plasma

  19. Kapelouzou A., Tsourelis L., Kaklamanis L., et al. Serum and tissue biomarkers in aortic stenosis. Glob Cardiol Sci Pract. 2015 Nov 13;2015(4):49. doi: 10.5339/gcsp.2015.49
    Species: Human
    Sample Type: Serum

  20. Acar B., Nese, Z., et al. Serum MMP-2, MMP-9, TIMP-1 and TIMP-2 levels in multiple sclerosis clinical subtypes and their diagnostic value in the progressive disease course. Biomedical Research, Volume 25, Issue 3, Pages 343 - 350. 2014.
    Species: Human
    Sample Type: Serum

  21. Domienik-Karłowicz J., et al. Emerging Markers of Atherosclerosis Before and After Bariatric Surgery. Obesity Surgery Sept 2014. epub ahead of print. DOI:10.1007/s11695-014-1407-7
    Species: Human
    Sample Type: Serum

  22. Esa S., et al. Study of the level of sputum matrix metalloproteinase-9 (MMP-9) and tissue inhibitor metalloproteinase-1 (TIMP-1) in COPD patients. Egyptian Journal of Chest Diseases and Tuberculosis Available online 18 July 2014 DOI: 10.1016/j.ejcdt.2014.06.014
    Species: Human
    Sample Type: Sputum

  23. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
    Species: Human
    Sample Type: Plasma

  24. Gluba-Brzozka A., et al. Markers of increased cardiovascular risk in patients with chronic kidney disease. Lipids in Health and Disease 2014, 13:135 doi:10.1186/1476-511X-13-135
    Species: Human
    Sample Type: Serum

  25. Kılıç Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anadolu Kardiyol Derg 2014; 14: 531-41. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample Type: Serum

  26. Kilic Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anatolian J of Card. Published ahead of print, June 25th 2014. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample Type: Serum

  27. Kulkarni P., Haldar M., Nahire R., Katti P., Ambre A., Muhonen W., Shabb J., et al. MMP-9 Responsive PEG Cleavable Nanovesicles for Efficient Delivery of Chemotherapeutics to Pancreatic Cancer. Mol. Pharmaceutics. (2014)
    Species: Human
    Sample Type: Conditioned Media

  28. Yang H, Wang B, Wang T, Xu L, He C, et al. (2014) Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via LipopolysaccharideStimulation and Is Overexpressed in Patients with Lymph Node Metastasis. PLoS ONE 9(10): e109980. doi:10.1371/journal.pone.0109980
    Species: Human
    Sample Type: Conditioned Media

  29. Guzel S, Serin O, Guzel EC, et al. Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarction. Korean J Intern Med. 2013 Mar;28(2):165-173.
    Species: Human
    Sample Type: Serum

  30. Khadjavi A, Valente E, Giribaldi G, Prato M. Involvement of p38 MAPK in haemozoin-dependent MMP-9 enhancement in human monocytes. Cell Biochem. Funct. 2013; Epub ahead of print, Mar 7, 2013; doi: 10.1002/cbf.2963
    Species: Human
    Sample Type: Conditioned Media

  31. Slawinska-Brych A, Zdzisinska B, Mizerska-Dudka M, Kandefer-Szerszen M. Induction of apoptosis in multiple myeloma cells by a statin-thalidomide combination can be enhanced by p38 MAPK inhibition. Leuk Res. 2013 May;37(5):586-594.
    Species: human
    Sample Type: Conditioned Media

  32. Chim H., Armijo BS., Miller E., et al. Propranolol induces regression of hemangioma cells through HIF-1α-mediated inhibition of VEGF-A. Ann Surg. 2012 Jul;256(1):146-56. doi: 10.1097/SLA.0b013e318254ce7a.
    Species: Human
    Sample Type: Conditioned Media

  33. Kilickesmez KO., et al. Dilatation of the ascending aorta and serum alpha 1-antitrypsin level in patients with bicuspid aortic valve. Heart Vessels (2012) 27:391-397 DOI 10.1007/s00380-011-0161-1
    Species: Human
    Sample Type: Serum

  34. Sundrani DP, Chavan-Gautam PM, Pisal HR, Mehendale SS, Joshi SR (2012) Matrix Metalloproteinase-1 and -9 in Human Placenta during SpontaneousVaginal Delivery and Caesarean Sectioning in Preterm Pregnancy. PLoS ONE 7(1): e29855. doi:10.1371/journal.pone.0029855
    Species: Human
    Sample Type: Tissue Lysate

  35. Zhu P., Ren M., Yang C., et al. Involvement of RAGE, MAPK and NF-κ pathways in AGEs-induced MMP-9 activation in HaCaT keratinocytes. Exp Dermatol. 2012 Feb;21(2):123-9. doi: 10.1111/j.1600-0625.2011.01408.x.
    Species: Human
    Sample Type: Conditioned Media

  36. Cherneva, RV. Et al. The role of small heat-shock protein αB-crystalline (HspB5) in COPD pathogenesis. Int J Chron Obstruct Pulmon Dis. 2012;7:633-40. doi: 10.2147/COPD.S34929. Epub 2012 Oct 4.
    Species: Human
    Sample Type: Plasma

  37. Fuchs S., Lavi I., Tzang O., et al. Intracoronary monocyte chemoattractant protein 1 and vascular endothelial growth factor levels are associated with necrotic core, calcium and fibrous tissue atherosclerotic plaque components: an intracoronary ultrasound radiofrequency study. Cardiology. 2012;123(2):125-32. doi: 10.1159/000342050.
    Species: Human
    Sample Type: Serum

  38. Wyrebska A., Gach K., Szemraj J., et al. Comparison of anti-invasive activity of parthenolide and 3-isopropyl-2-methyl-4-methyleneisoxazolidin-5-one (MZ-6)--a new compound with α-methylene-γ-lactone motif--on two breast cancer cell lines. Chem Biol Drug Des. 2012 Jan;79(1):112-20. doi: 10.1111/j.1747-0285.2011.01257.x.
    Species: Human
    Sample Type: Conditioned Media

  39. Kaminska-Winciorek G., Deja G., Polanska J., Jarosz-Chobot P. The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study. Int J Clin Pract. 2012 Apr;66(4):374-7. doi: 10.1111/j.1742-1241.2011.02702.x.
    Species: Human
    Sample Type: Serum

  40. Leng L. Jiang T., Zhang Y. TLR9 expression is associated with prognosis in patients with glioblastoma multiforme. J Clin Neurosci. 2012 Jan;19(1):75-80. doi: 10.1016/j.jocn.2011.03.037.
    Species: Human
    Sample Type: Conditioned Media

  41. Dabrowski W., Rzecki Z., Czajkowski M., et al. Volatile anesthetics reduce biochemical markers of brain injury and brain magnesium disorders in patients undergoing coronary artery bypass graft surgery. J Cardiothorac Vasc Anesth. 2012 Jun;26(3):395-402. doi: 10.1053/j.jvca.2011.10.014
    Species: Human
    Sample Type: Serum

  42. Jacob J., Eisemon E., Sheibani-Rad S., et al. Matrix metalloproteinase levels as a marker for rotator cuff tears. Orthopedics. 2012 Apr;35(4):e474-8. doi: 10.3928/01477447-20120327-18.
    Species: Human
    Sample Type: Tissue Lysate

  43. Yilmax A., Bilge I., Kiyak A., Gedikbasi A., et al. Matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in vesicoureteral reflux. Pediatr Nephrol. 2012 Mar;27(3):435-41. doi: 10.1007/s00467-011-2026-3.
    Species: Human
    Sample Type: Urine

  44. Jung J., Jung K., Kim D., Kim H. Selective inhibition of MMP-9 gene expression by mangiferin in PMA-stimulated human astroglioma cells: involvement of PI3K/Akt and MAPK signaling pathways. Pharmacol Res. 2012 Jul;66(1):95-103. doi: 10.1016/j.phrs.2012.02.013.
    Species: Human
    Sample Type: Conditioned Media

  45. Prasadm I., Crawford R., Xiao Y. Aggravation of ADAMTS and matrix metalloproteinase production and role of ERK1/2 pathway in the interaction of osteoarthritic subchondral bone osteoblasts and articular cartilage chondrocytes -- possible pathogenic role in osteoarthritis. J Rheumatol. 2012 Mar;39(3):621-34. doi: 10.3899/jrheum.110777
    Species: Human
    Sample Type: Conditioned Media

  46. Baalash A., Hamouda H., Ismail G., et al. The Role of Heat Shock Protein 70, IgE and MMP-9 in Detecting Early Minor Myocardial Damage and Evaluating the Efficacy of Coronary Artery Bypass Grafting (CABG). Journal of Life Sciences 6 (2012) 260-267
    Species: Human
    Sample Type: Serum

  47. Aslan H., et al. Serum matrix metalloproteinases and tympanosclerosis. The Journal of Laryngology & Otology (2011), 125, 142-146. DOI:10.1017/S002221511000201X
    Species: Human
    Sample Type: Serum

  48. Gach, K., Szemraj, J., Wyrębska, A. et al. Mol Biol Rep (2011) 38: 1231. doi:10.1007/s11033-010-0222-z
    Species: Human
    Sample Type: Conditioned Media

  49. Zhu, Ping, et al. "Impairment of human keratinocyte mobility and proliferation by advanced glycation end products-modified BSA." Archives of dermatological research 303.5 (2011): 339-350.

  50. Yigit, Özgür, et al. "The effect of corticosteroid on metalloproteinase levels of nasal polyposis 1." The Laryngoscope 121.3 (2011): 667-673.

  51. Yigit, Özgür, et al. "The effect of corticosteroid on metalloproteinase levels of nasal polyposis 1." The Laryngoscope 121.3 (2011): 667-673.

  52. Amon L., Law W., Fitzgibbon M., et al. Integrative proteomic analysis of serum and peritoneal fluids helps identify proteins that are up-regulated in serum of women with ovarian cancer. PLoS One. 2010 Jun 15;5(6):e11137. doi: 10.1371/journal.pone.0011137.
    Species: Human
    Sample Type: Serum

  53. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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