Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Name: Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit
SKU: CBEL-Stat-SK
Availability: InStock

RayBio® Cell-Based Human/Mouse/Rat Stat (1, 3, and 5) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Product Description

Specifications

Size	2 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human, Mouse, Rat
Specificity	The antibodies provided in this kit recognizes human, mouse and rat STAT 1 phosphorylated at site Tyrosine-701, STAT 3 phosphorylated at site Tyrosine-705, and STAT 5 phosphorylated at site Tyrosine-694. This kit also recognizes total Stat 1, total Stat2 and total Stat 3 for comparison.
Compatible Sample Types	Adherent Cell Culture
Protein Name / Synonyms	Signal transducer and activator of transcription 1-alpha/beta (Transcription factor ISGF-3 components p91/p84)/Signal transducer and activator of transcription 3 (Acute-phase response factor)/Signal transducer and activator of transcription 5A
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Cell-based
Method Of Detection	Colorimetric
Research Area	Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Site and signal pathway-specific
In vitro detection of adherent cell culture
No sample lysis needed
Compatible with a standard ELISA plate reader
Faster results than with ELISA
Adaptable for high-throughput screening and drug discovery

Application Notes

Kit Components

Cell Culture Microplate
Wash Buffer A
Wash Buffer B
Fixing Solution
HRP-Conjugated Secondary Antibody
Blocking Buffer
TMB One-Step Substrate
Stop Solution
Quenching Buffer

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 °C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

-20°C

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Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (CBEL-Stat-SK.pdf)

SDS (387da446f4406dd513cd8d90c1550ea36a99197c.pdf)

Product Features

Site and signal pathway-specific
In vitro detection of adherent cell culture
No sample lysis needed
Compatible with a standard ELISA plate reader
Faster results than with ELISA
Adaptable for high-throughput screening and drug discovery

Application Notes

Kit Components

Cell Culture Microplate
Wash Buffer A
Wash Buffer B
Fixing Solution
HRP-Conjugated Secondary Antibody
Blocking Buffer
TMB One-Step Substrate
Stop Solution
Quenching Buffer

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 °C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

-20°C

Featured Products

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Featured Products

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit

Human/Mouse/Rat Phospho-Stat (1, 3, and 5) Cell-Based ELISA Kit