Human Neurokinin-A EIA

$376.00
EIA-NEA-1

RayBio® Human/Mouse/Rat Neurokinin-A EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.

Introduction

Neurokinin A, aka substance K, is a peptide hormone belonging to the tachykinin family, which also includes Substance P and Neurokinin B. All the peptides from tachykinin families are derived from two preprotachykinin genes - the PPT-A gene and PPT-B gene. The former encodes the sequences of Substance P. Neurokinin A. and Neuropeptide K and the latter encodes the sequence of Neurokinin B. Neuropeptide K and Neuropeptide gamma are N-terminally longer versions of neurokinin A.

Neurokinin A has played a variety of roles in human. For example, it is reported that Neurokinin A may mediate bronchoconstriction through the non-adrenergic non-cholinergic nervous system in rat.

There has been no clinical application reported for neurokinin A.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

The capture antibody provided in this kit recognizes human, mouse and rat Neurokinin-A.

Target Protein Information

Synonyms
Protachykinin-1 (PPT) [Cleaved into: Neurokinin A (NKA) (NKNA) (Neuromedin L) (Substance K)]
Accession Number
Gene Names
TAC1 NKA NKNA TAC2
Gene ID
6863

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.
  • Storage/Stability

    Standard, Biotinylated Neurokinin A peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

    Antigen Information

    Gene Symbol:
    • TAC1

    Product Specifications

    Size:
    1, 2, or 5 x 96-Well Strip Microplate Kit

    Array/ELISA Format

    Species Detected:
    • Human
    • Mouse
    • Rat
    Solid Support:
    96-well Microplate
    Design Principle:
    • Competition-based
    Quantitative/Semi-Quantitative:
    Quantitative
    Method of Detection:
    Colorimetric
    Compatible Sample Types:
    • Serum
    • Cell Culture Supernatants
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    Usage

    This product is furnished for LABORATORY RESEARCH USE ONLY.
    Not for diagnostic or therapeutic use.

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