Human NT-3 ELISA

RayBio® Human NT-3 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-NT3-1
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Antigen Information

Accession Number:
  • P20783
Gene ID:
  • 4908
Gene Symbols:
  • NTF3
Protein Name & Synonyms:
Neurotrophin-3 (NT-3) (HDNF) (Nerve growth factor 2) (NGF-2) (Neurotrophic factor)
Target Species:
  • Human

Assay Format

Detection Range:
4 pg/ml - 3000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
4 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wysokinski A., Serum levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in depressed patients with schizophrenia. Nord J Psychiatry. 2016;70(4):267-71. doi: 10.3109/08039488.2015.1087592
    Species: Human
    Sample type: Serum
  2. Betz V., Sitoci-Ficici K., Uckermann O., et al. Gene-activated fat grafts for the repair of spinal cord injury: a pilot study. Acta Neurochir (Wien). 2016 Feb;158(2):367-78. doi: 10.1007/s00701-015-2626-y.
    Species: Rat
    Sample type: Conditioned Media
  3. Priya P., Rajappa M., Kattimani S., et al. Association of neurotrophins, inflammation and stress with suicide risk in young adults. Clin Chim Acta. 2016 Jun 1;457:41-5. doi: 10.1016/j.cca.2016.03.019.
    Species: Human
    Sample type: Serum
  4. Sun J., Mandai M., Kamao H., et al. Protective Effects of Human iPS-Derived Retinal Pigmented Epithelial Cells in Comparison with Human Mesenchymal Stromal Cells and Human Neural Stem Cells on the Degenerating Retina in rd1 mice. Stem Cells. 2015 May;33(5):1543-53. doi: 10.1002/stem.1960.
    Species: Human
    Sample type: Conditioned Media
  5. Munkholm K., Klarlund B., Lars P., Kessing V., Vinberg M. Elevated levels of plasma brain derived neurotrophic factor in rapid cycling bipolar disorder patient. Pyschoneuroendocrinology, 2014 Epub ahead of print, http://dx.doi.org/doi:10.1016/j.psyneuen.2014.05.011
    Species: Human
    Sample type: Plasma
  6. Weishaupt, N., et al. "Synergistic effects of BDNF and rehabilitative training on recovery after cervical spinal cord injury." Behavioural brain research 239 (2013): 31-42.
    Species: Human
    Sample type: Cell Lysate
  7. Kalinowska-Lyszczarz A., Pawlak M., Michalak S., Losy J. Cognitive deficit is related to immune-cell beta-NGF in multiple sclerosis patients. J Neurol Sci. 2012 Oct 15;321(1-2):43-8. doi: 10.1016/j.jns.2012.07.044
    Species: Human
    Sample type: Cell Lysate
  8. Çelebi, Betül, Diego Mantovani, and Nicolas Pineault. "Irradiated mesenchymal stem cells improve the ex vivo expansion of hematopoietic progenitors by partly mimicking the bone marrow endosteal environment." Journal of immunological methods 370.1 (2011): 93-103.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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