Human Osteopontin (OPN) ELISA


RayBio® Human Osteopontin (SPP1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

50 pg/ml

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Detection Range
50 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma)
3 - 100 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data


This ELISA kit shows no cross-reactivity with any of the following cytokines tested (human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Protein Name & Synonyms
Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin)
Accession Number
Gene Names
Gene ID

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • SPP1

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Method of Detection:
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample Type: Serum

  2. Filis K., Martinakis V., Galyfos G., Sigala F., Theodorou D., Andreadou I., Zografos G. OSTEOPONTIN AND OSTEOPROTEGERIN AS POTENTIAL BIOMARKERS IN ABDOMINAL AORTIC ANEURYSM BEFORE AND AFTER TREATMENT. International Scholarly Research Notices, 2014 May, Epub Ahead of Print
    Species: Human
    Sample Type: Serum

  3. Gluba-Brzozka A., et al. Markers of increased cardiovascular risk in patients with chronic kidney disease. Lipids in Health and Disease 2014, 13:135 doi:10.1186/1476-511X-13-135
    Species: Human
    Sample Type: Serum

  4. Hussein Y., Alhazmi A., Alzahrani S., et al. Osteopontin as a Marker for Response to Pegylated Interferon Alpha-κ Treatment in Chronic HCV Saudi Patients. Vol 21, No. 12;Dec 2014. 445 - 454
    Species: Human
    Sample Type: Serum

  5. Bassyouni IH, El-Wakd MM, Bassyouni RH. Soluble levels of osteopontin in patients with Behcet’s disease: association with disease activity and vascular involvement. J Clin Immunol. 2013 Feb;33(2):361-367.
    Species: Human
    Sample Type: Plasma

  6. Hafez SS, El Sebai AA, Al Aziz MM, et al. Alfa-Fetoprotein L3 Subfraction and Osteopontin: Novel Markers for the Diagnosis of Hepatocellular Carcinoma. J Am Sci. 2013;9(3).
    Species: Human
    Sample Type: Serum

  7. Nagy E, Eriksson P, Yousry M, et al. Valvular osteoclasts in calcification and aortic valve stenosis severity. Int J Cardiol. 2013 Feb 27; [Epub ahead of print] doi:10.1016/j.ijcard.2013.01.207.
    Species: Human
    Sample Type: Plasma

  8. Abdul Wahab Ali K., Abdulbari A., Hamdi R. Determination of Procollagen 1 N Terminal Peptide and Osteopontin in Postmenopausal Women with Vertebral Osteoporotic Fractures. KASR EL AINI MEDICAL JOURNAL VOL. 18, NO 3, July 2012
    Species: Human
    Sample Type: Serum

  9. Von Neuhoff N, Oumeraci T, Wolf T, et al. Monitoring CSF Proteome Alterations in Amyotrophic Lateral Sclerosis: Obstacles and Perspectives in Translating a Novel Marker Panel to the Clinic. Borchelt DR, ed. PLoS ONE. 2012;7(9):e44401. doi:10.1371/journal.pone.0044401.
    Species: Human
    Sample Type: CSF

  10. Taranta-Janusz K., Wasilewska A., Stypulkowska J., Sutula M. Osteopontin and symmetric dimethylarginine plasma levels in solitary functioning kidney in children. Acta Paediatr. 2012 Aug;101(8):e369-72. doi: 10.1111/j.1651-2227.2012.02690.x
    Species: Human
    Sample Type: Plasma

  11. Bassyouni I., Bassyouni R., Ibrahim N., Soliman A. Elevated serum osteopontin levels in chronic hepatitis C virus infection: association with autoimmune rheumatologic manifestations. J Clin Immunol. 2012 Dec;32(6):1262-9. doi: 10.1007/s10875-012-9727-7.
    Species: Human
    Sample Type: Serum

  12. Acar A., Cevik M., Arikanoglu A., et al. Serum levels of calcification inhibitors in patients with intracerebral hemorrhage. Int J Neurosci. 2012 May;122(5):227-32. doi: 10.3109/00207454.2011.642039.
    Species: Human
    Sample Type: Serum

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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