Human Osteoprotegerin (OPG) ELISA

$398.00
ELH-OPG-1

RayBio® Human Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
1 pg/ml

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Detection Range
1 pg/ml - 900 pg/ml
Recommended Dilution (serum & plasma)
2 - 10 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Tumor necrosis factor receptor superfamily member 11B (Osteoclastogenesis inhibitory factor) (Osteoprotegerin)
Accession Number
Gene Names
TNFRSF11B, OCIF, OPG
Gene ID
4982

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • TNFRSF11B

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Abdallah E., El-Shishtawy S., Sherif N., Abdelwahab M. Diagnostic Performance of Urinary Osteoprotegrin as A Novel Biomarker for Early Detection of Lupus Nephritis Activity. Life Science Journal 2015;12(1) 75-81]. (ISSN:1097-8135)
    Species: Human
    Sample Type: Urine

  2. Wang J., Sun X., Zhang H., Wang Y., Li Y. MPA influences tumor cell proliferation, migration, and invasion induced by RANKL through PRB involving the MAPK pathway in endometrial cancer. Oncol Rep. 2015 Feb;33(2):799-809. doi: 10.3892/or.2014.3651
    Species: Human
    Sample Type: Serum

  3. KOÇYİĞİT I.; TÜRKMEN K; DOĞAN E., et al. The Relationship Between Osteoprotegerin/RANKL Axis and Arterial Stiffness in Osteopenic/Osteoporotic Renal Transplantation Recipients. Turk Neph Dial Transpl 2015; 24 (2): 181-188 doi: 10.5262/tndt.2015.1002.07
    Species: Human
    Sample Type: Serum

  4. Bakry R., El-Sayed M., Hamza H., Hassan K. Pretreatment levels of serum osteoprotegerin and p53 protein and urine telomerase as prognostic factors affecting survival in Egyptian bladder cancer patients. Oncol Lett. 2016 Jan;11(1):823-830. Epub 2015 Nov 17.
    Species: Human
    Sample Type: Serum

  5. Kapelouzou A., Tsourelis L., Kaklamanis L., et al. Serum and tissue biomarkers in aortic stenosis. Glob Cardiol Sci Pract. 2015 Nov 13;2015(4):49. doi: 10.5339/gcsp.2015.49
    Species: Human
    Sample Type: Serum

  6. Filis K., Martinakis V., Galyfos G., Sigala F., Theodorou D., Andreadou I., Zografos G. OSTEOPONTIN AND OSTEOPROTEGERIN AS POTENTIAL BIOMARKERS IN ABDOMINAL AORTIC ANEURYSM BEFORE AND AFTER TREATMENT. International Scholarly Research Notices, 2014 May, Epub Ahead of Print
    Species: Human
    Sample Type: Serum

  7. Holt V, Caplan AI, Haynesworth SE (2014) Identification of a Subpopulation of Marrow MSC-Derived Medullary Adipocytes That Express Osteoclast- Regulating Molecules: Marrow Adipocytes Express Osteoclast Mediators. PLoS ONE 9(10): e108920. doi:10.1371/journal.pone.0108920
    Species: Human
    Sample Type: Conditioned Media

  8. Lucas R., Ramos E., Prata M., Rodrigues A., Costa L., et al. Changes in serum RANKL and OPG with sexual development and their associations with bone turnover and bone mineral density in a cohort of girls. Clinical Biochemistry Available online 22 April 2014 [Epub ahead of Print]
    Species: Human
    Sample Type: Serum

  9. MacMillan AK., Lamberti FV., Moulton JN., et al. Similar healthy osteoclast and osteoblast activity on nanocrystalline hydroxyapatite and nanoparticles of tri-calcium phosphate compared to natural bone. Int J Nanomedicine. 2014; 9: 5627-5637.
    Species: Human
    Sample Type: Nanomaterial

  10. Scher J., et al. Decreased Bacterial Diversity Characterizes an Altered Gut Microbiota in Psoriatic Arthritis and Resembles Dysbiosis of Inflammatory Bowel Disease. Arthritis and Rhuematology November 2014, epub ahead of print. DOI 10.1002/art.38892
    Species: Human
    Sample Type: Stool

  11. MacMillan AK., Lamberti FV., Moulton JN., et al. Similar healthy osteoclast and osteoblast activity on nanocrystalline hydroxyapatite and nanoparticles of tri-calcium phosphate compared to natural bone. Int J Nanomedicine. 2014; 9: 5627-5637.
    Species: Human
    Sample Type: Tissue Lysate

  12. Akelma AZ, Cizmeci MN, Kanburoglu MK, Bozhkaya D, Catal F, et al. Elevated level of serum osteopontin in school-age children with asthma. Allergol Immunop (Madr). 2013; Published online ahead of print, April 8, 2013. doi:10.1016/j.aller.2013.01.009
    Species: Human
    Sample Type: Serum

  13. Hartwich JE, Orr WS, Ng CY, et al. Rapamycin increases neuroblastoma xenograft and host stromal derived osteoprotegerin inhibiting osteolytic bone disease in a bone metastasis model. J Pediatr Surg. 2013 Jan;48(1):47-55.
    Species: Mouse
    Sample Type: Serum

  14. Nishigaki M, Yamamoto T, Ichioka H, et al. β-cryptoxanthin regulates bone resorption related-cytokine production in human periodontal ligament cells. Arch Oral Biol. 2013 Jul;58(7):880-886.
    Species: Human
    Sample Type: Conditioned Media

  15. Palumbo S, Li W-J. Osteoprotegerin Enhances Osteogenesis of Human Mesenchymal Stem Cells. Tissue Eng Part A. Volume 19, Numbers 19 and 20, 2013, DOI: 10.1089/ten.tea.2012.0550
    Species: Human
    Sample Type: Conditioned Media

  16. Pamuk BO, Sari I, Selcuk S, et al. Evaluation of circulating endothelial biomarkers in familial Mediterranean fever. Rheumatol Int. 2013 Aug;33(8):1967-1972.
    Species: Human
    Sample Type: Serum

  17. Erkol A., Oduncu V., Pala S., et al. Plasma osteoprotegerin level on admission is associated with no-reflow phenomenon after primary angioplasty and subsequent left ventricular remodeling in patients with acute ST-segment elevation myocardial infarction. Atherosclerosis. 2012 Mar;221(1):254-9. doi: 10.1016/j.atherosclerosis.2011.12.031.
    Species: Human
    Sample Type: Plasma

  18. Zheng C., Chu P., Wu C., et al. Association between increased serum osteoprotegerin levels and improvement in bone mineral density after parathyroidectomy in hemodialysis patients. Tohoku J Exp Med. 2012;226(1):19-27.
    Species: Human
    Sample Type: Serum

  19. Attia, Enas AS, et al. "Assessment of osteoporosis in psoriasis with and without arthritis: correlation with disease severity." International journal of dermatology 50.1 (2011): 30-35.

  20. Tang, T., et al. "Effect of water-soluble P-chitosan and S-chitosan on human primary osteoblasts and giant cell tumor of bone stromal cells." Biomedical Materials 6.1 (2011): 015004.

  21. Erkol, Ayhan, et al. "Relation of circulating osteoprotegerin levels on admission to microvascular obstruction after primary percutaneous coronary intervention." The American journal of cardiology 107.6 (2011): 857-862.

  22. Mohamed JA, DuPont HL, Jiang ZD, Flores J, Carlin LG, Belkind-Gerson J, et al. A Single-Nucleotide Polymorphism in the Gene Encoding Osteoprotegerin, an Anti-inflammatory Protein Produced in Response to Infection with Diarrheagenic Escherichia coli, Is Associated with an Increased Risk of Nonsecretory Bacterial Diarrhea in North American Travelers to Mexico. J Infect Dis. 2009;199:477-485.
    Species: Human
    Sample Type: Conditioned Media

  23. Mohamed JA, DuPont HL, Jiang ZD, Flores J, Carlin LG, Belkind-Gerson J, et al. A Single-Nucleotide Polymorphism in the Gene Encoding Osteoprotegerin, an Anti-inflammatory Protein Produced in Response to Infection with Diarrheagenic Escherichia coli, Is Associated with an Increased Risk of Nonsecretory Bacterial Diarrhea in North American Travelers to Mexico. J Infect Dis. 2009;199:477-485.
    Species: Human
    Sample Type: Stool

  24. Jose´ I Botella-Carretero, Francisco Alvarez-Blasco, Jose´ Luis San Milla´n and He´ctor F Escobar-Morreale. Thyroid hormone deficiency and postmenopausal status independently increase serum osteoprotegerin concentrations in women. Eur J Endocrinol. 2007;156:539-545
    Species: Human
    Sample Type: Serum

  25. Maksymowych WP, Landewe R, Conner-Spady B, Dougados, et al. Serum Matrix Metalloproteinase 3 Is an Independent Predictor of Structural Damage Progression in Patients with Ankylosing Spondylitis. Arthritis Rheum. 2007;56(6):1846-1853.
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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