Human PDGF-BB ELISA

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RayBio® Human PDGF-BB ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-PDGFBB-1
+

Antigen Information

Accession Number:
  • P01127
Gene ID:
  • 5155
Gene Symbols:
  • PDGF2
  • PDGFB
  • SIS
Protein Name & Synonyms:
Platelet-derived growth factor subunit B (PDGF subunit B) (PDGF-2) (Platelet-derived growth factor B chain) (Platelet-derived growth factor beta polypeptide) (Proto-oncogene c-Sis) (Becaplermin)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Gamal A., Abdel-Ghaffer K., Iacono V.Gingival crevicular fluid vascular endothelial cell growth factor and platelet-derived growth factor-BB release profile following the use of perforated barrier membranes during treatment of intrabony defects: a randomized clinical trial.J Periodontal Res. 2016 Jun;51(3):407-16. doi: 10.1111/jre.12321
    Species: Human
    Sample type: Gingival Crevicular Fluid
  2. Wu Y., Kanna M., Liu C., et al. Generation of Autologous Platelet-Rich Plasma by the Ultrasonic Standing Waves. IEEE Trans Biomed Eng. 2015 Jun 25.
    Species: Human
    Sample type: Plasma
  3. Muraglia A., Todeschi M., Papait A., et al. Combined platelet and plasma derivatives enhance proliferation of stem/progenitor cells maintaining their differentiation potential. Cytotherapy. 2015 Dec;17(12):1793-806. doi: 10.1016/j.jcyt.2015.09.004.
    Species: Human
    Sample type: Conditioned Media
  4. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  5. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample type: Tissue Lysate
  6. Lima AC., et al. Fast and Mild Strategy, Using Superhydrophobic Surfaces, to Produce Collagen/Platelet Lysate Gel Beads for Skin Regeneration. Stem Cell Rev and Rep, Aug 2014. epub ahead of print. DOI 10.1007/s12015-014-9548-6
    Species: Human
    Sample type: Conditioned Media
  7. Stojek M., et al. Decreased serum platelet derived growth factor BB levels in acute and increased in chronic pancreatitis. World J Gastroenterol 2014 September 28; 20(36): 13127-13132. doi: 10.3748/wjg.v20.i36.13127
    Species: Human
    Sample type: Serum
  8. Stoken M., et al. Decreased serum platelet derived growth factor BB levels in acute and increased in chronic pancreatitis. World Journal of GastroenterologyESPS Manuscript NO: 10488 Published Online July 2014 http://www.wjgnet.com/esps/DownloadFile.aspx%3FType%3DM%26MsId%3D10488%26FilePath%3DUserFiles%255CManuscript%255C00010635%255C10488-Review.docx
    Species: Human
    Sample type: Serum
  9. Winsz-Szczotka K., et al. Circulating keratan sulfate as a marker of metabolic changes of cartilage proteoglycan in juvenile idiopathic arthritis; influence of growth factors as well as proteolytic and prooxidative agents on aggrecan alterations. Clin Chem Lab Med 2014; aop DOI 10.1515/cclm-2014-0441
    Species: Human
    Sample type: Plasma
  10. Cadamuro M, Nardo G, Indraccolo S, et al. Platelet-derived growth factor-D and Rho GTPases regulate recruitment of cancer-associated fibroblasts in cholangiocarcinoma. Hepatology. 2013 Sep;58(3):1042-1053.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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