Human Procalcitonin ELISA

$398.00
ELH-PROCALC-1

RayBio® Human Procalcitonin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
30 pg/ml

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Detection Range
30 pg/ml - 20000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Calcitonin
Accession Number
Gene Names
CALCA, CALC1
Gene ID
796

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CALCA

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Basiri R., Hadizadeh Talasaz Z., Tohidi M., et al. Evaluation of the diagnostic values of pleural fluid procalcitonin in transudates and exudates pleural effusions. Journal of Chemical and Pharmaceutical Research, 2015, 7(2):10-15
    Species: Human
    Sample Type: Serum

  2. Basiri R., Hadizadeh Talasaz Z., Tohidi M., et al. Evaluation of the diagnostic values of pleural fluid procalcitonin in transudates and exudates pleural effusions. Journal of Chemical and Pharmaceutical Research, 2015, 7(2):10-15
    Species: Human
    Sample Type: Pleural Fluid

  3. Dabbous H., Ali-Eldin F., Montasser I. Role of procalcitonin in diagnosis of bacterial infection in trans-arterial chemoembolisation treated hepatocellular carcinoma patients. Arab J Gastroenterol. 2015 Mar;16(1):10-3. doi: 10.1016/j.ajg.2015.03.001.
    Species: Human
    Sample Type: Serum

  4. Dulay A., Buhimschi I., Guomao Z., et al. Compartmentalization of acute phase reactants Interleukin-6, C-Reactive Protein and Procalcitonin as biomarkers of intra-amniotic infection and chorioamnionitis. Cytokine. 2015 Dec;76(2):236-43. doi: 10.1016/j.cyto.2015.04.014.
    Species: Human
    Sample Type: Serum

  5. Dulay A., Buhimschi I., Guomao Z., et al. Compartmentalization of acute phase reactants Interleukin-6, C-Reactive Protein and Procalcitonin as biomarkers of intra-amniotic infection and chorioamnionitis. Cytokine. 2015 Dec;76(2):236-43. doi: 10.1016/j.cyto.2015.04.014.
    Species: Human
    Sample Type: Cord Blood

  6. Dulay A., Buhimschi I., Guomao Z., et al. Compartmentalization of acute phase reactants Interleukin-6, C-Reactive Protein and Procalcitonin as biomarkers of intra-amniotic infection and chorioamnionitis. Cytokine. 2015 Dec;76(2):236-43. doi: 10.1016/j.cyto.2015.04.014.
    Species: Human
    Sample Type: Amniotic Fluid

  7. Dulay A., Buhimschi I., Guomao Z., et al. Compartmentalization of acute phase reactants Interleukin-6, C-Reactive Protein and Procalcitonin as biomarkers of intra-amniotic infection and chorioamnionitis. Cytokine. 2015 Dec;76(2):236-43. doi: 10.1016/j.cyto.2015.04.014.
    Species: Human
    Sample Type: Urine

  8. El-Shafey B., Bahr H., Ganna S., et al. The diagnostic value of serum levels of C-reactive protein and procalcitonin in differentiation between active pulmonary TB and CAP. Egyptian Journal of Bronchology. Year : 2015, Volume : 9, Issue : 2, Page : 178-182, DOI: 10.4103/1687-8426.158071
    Species: Human
    Sample Type: Serum

  9. Arora S., Singh P., Sing PM., Trikha A. Procalcitonin levels in survivors and non survivors of Sepsis: Systematic review and Meta-analysis. Shock 2014 Nov 24, DOI: 10.1097/SHK.0000000000000305
    Species: Human
    Sample Type: Serum

  10. Jain S., et al. Procalcitonin as a prognostic marker for sepsis: aprospective observational study. BMC Research Notes 2014, 7:458
    Species: Human
    Sample Type: Serum

  11. Prasad R, Kapoor R, Mishra OP, et al. Serum procalcitonin in septic meningitis. Indian J Pediatr. 2013 May;80(5):365-370.
    Species: Human
    Sample Type: Serum

  12. Bipath P., Viljoen M., Levay P.Levels of procalcitonin, C-reactive protein and neopterin in patients with advanced HIV-1 infection. SAJHIVMED June 2012, Vol. 13, No. 2
    Species: Human
    Sample Type: Plasma

  13. Mutluoglu M., et al. Can procalcitonin predict bone infection in diabetic persons with infected foot ulcers? A pilot study. Diabetes Res Clin Pract. 2011 Oct;94(1):53-6. doi: 10.1016/j.diabres.2011.05.023
    Species: Human
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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