Human RAGE ELISA

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$321.60
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RayBio® Human RAGE ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-RAGE-1
+

Antigen Information

Accession Number:
  • Q9UQ07
Gene ID:
  • 5891
Gene Symbols:
  • MOK
  • RAGE
  • RAGE1
Protein Name & Synonyms:
MAPK/MAK/MRK overlapping kinase (EC 2.7.11.22) (MOK protein kinase) (Renal tumor antigen 1) (RAGE-1)
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 1500 pg/ml
Recommended Dilution (serum & plasma):
2 - 20 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Bediwy A., Hussan S., El-Najjar M. Receptor of advanced glycation end products in childhood asthma exacerbation. Egyptian Journal of Chest Diseases and Tuberculosis. Volume 65, Issue 1, January 2016, Pages 15-18
    Species: Human
    Sample type: Serum
  2. Nirala B., Gohil NK. EFFECT OF GLYCATED SERUM ALBUMIN ON FUNCTIONAL MARKERS IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS IN THE PRESENCE OF SHEAR STRESS. J. Mech. Med. Biol. DOI: 10.1142/S0219519415500268
    Species: Human
    Sample type: Cell Lysate
  3. Birala BK., Perumal V., Gohil NK. Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction. Diab Vasc Dis Res. 2015 Jul;12(4):290-7. doi: 10.1177/1479164115583192.
    Species: Human
    Sample type: Conditioned Media
  4. Nirala B., Gohil N. Effect of garlic component s-allyl cysteine sulfoxide on glycated human serum albumin induced activation of endothelial cells: an in vitro study. Eur Rev Med Pharmacol Sci. 2015;19(11):2125-31.
    Species: Human
    Sample type: Cell Lysate
  5. Bala K., Gohil N. Interaction of glycated protein and DFO mimicked hypoxia in cellular responses of HUVECs. Mol Biosyst. 2012 Oct;8(10):2657-63. doi: 10.1039/c2mb25156f.
    Species: Human
    Sample type: Conditioned Media
  6. Chima, Ranjit S., et al. "C-peptide, a novel inhibitor of lung inflammation following hemorrhagic shock." American Journal of Physiology-Lung Cellular and Molecular Physiology 300.5 (2011): L730-L739.
    Species: Human
    Sample type: Plasma
  7. Bala, Kanchan, James Gomes, and Nivedita Karmakar Gohil. "Interaction of glycated human serum albumin with endothelial cells in a hemodynamic environment: structural and functional correlates." Molecular BioSystems 7.11 (2011): 3036-3041.
    Species: Human
    Sample type: Cell Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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