Human RANTES ELISA

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ELH-RANTES-1

RayBio® Human RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Antigen Information

Accession Number:
  • P13501
Gene ID:
  • 6352
Gene Symbols:
  • CCL5
  • D17S136E
  • SCYA5
Protein Name & Synonyms:
C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68) RANTES(4-68)]
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 - 50 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample type: Conditioned Media
  2. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF?7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016
    Species: Human
    Sample type: Conditioned Media
  3. Chen X, Eksioglu EA, Carter JD, Fortenbery N, Donatelli SS, Zhou J, et al. (2015) Inactivation of DAP12 in PMN Inhibits TREM1-Mediated Activation in Rheumatoid Arthritis. PLoS ONE 10(2): e0115116. doi:10.1371/journal.pone.0115116
    Species: Human
    Sample type: Conditioned Media
  4. Tuchscherr L, Bischoff M, Lattar SM, Noto Llana M, Pförtner H, Niemann S, et al. (2015) Sigma Factor SigB Is Crucial to Mediate Staphylococcus aureus Adaptation during Chronic Infections. PLoS Pathog 11(4): e1004870. doi:10.1371/journal. ppat.1004870
    Species: Human
    Sample type: Conditioned Media
  5. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample type: Serum
  6. Zemer-Wassercug N., Haim M., Leshem-Lev D., et al. The effect of dabigatran and rivaroxaban on platelet reactivity and inflammatory markers. J Thromb Thrombolysis. 2015 Oct;40(3):340-6. doi: 10.1007/s11239-015-1245-z.
    Species: Human
    Sample type: Plasma
  7. Israelow B., Narbus C., Sourisseau M., Evans M. HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection. Hepatology Epub Ahead of Print, doi: 10.1002/hep.27227
    Species: Human
    Sample type: Conditioned Media
  8. Lipkova, J., Parenica, J. et al. Association of circulating levels of RANTES and ?403G/A promoter polymorphism to acute heart failure after STEMI and to cardiogenic shock. Clinical and Experimental Medicine 2014. Epub Ahead of Print. DOI: 10.1007/s10238-014-0294-5
    Species: Human
    Sample type: Serum
  9. Parnell, Grant P., David R. Booth, and Dorothee Nickles. "The Autoimmune Disease-associated Transcription Factors EOMES and TBX21 Are Dysregulated in Multiple Sclerosis and Define a Molecular Subtype of Disease." Clinical Immunology. ScienceDirect, 15 Jan. 2014. Web.
    Species: Human
    Sample type: Serum
  10. Rajajendram R., Tham CL., Akhtar MN., et al.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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