• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 µL to 1 µL volumes
• Adjustable 1-25 µL pipettes for reagent preparation
• 100 µL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard and sample dilutions

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 µL Stop Solution to each well. Read at 450 nm immediately

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.