The RayBio® Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification Kit is a TaqMan™ probe-based duplex qPCR system with highly specific primer–probe sets to selectively amplify telomeric repeats and the mitochondrial ND1 gene while excluding nuclear genomic loci.
| Size | 100 tests, 200 tests, 500 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Sensitivity | 300 pg/reaction |
| Specificity | The non-human and non-amplification control samples have higher Cq values than human DNA or are undetermined, which means there is high specificity for mtDNA, TL and SCR amplification. |
| Compatible Sample Types | Extracted DNA |
| Quantitative/Semi-Quantitative | Quantitative |
| Research Area | Cardiovascular Disease, Cancer, Liver & Renal Function, Aging |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
Telomere length (TL) and mitochondrial DNA copy number (mtDNA-CN) are widely recognized molecular biomarkers of biological aging and all-cause mortality. Oxidative stress and DNA damage drive telomere attrition and mitochondrial dysfunction in parallel, linking both markers to cumulative cellular stress. Short telomeres suppress mitochondrial biogenesis and are strongly associated with cardiovascular disease, chronic kidney disease, cancer, and other age-related disorders. In contrast, the combined profile of short TL with elevated mtDNA-CN is emerging as a signature of accumulated damage accompanied by compensatory mitochondrial proliferation. Reliable, simultaneous measurement of TL and mtDNA-CN is therefore critical for aging research, preclinical drug development, and translational studies.
The RayBio® Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification Kit is a TaqMan™ probe-based duplex qPCR system with highly specific primer–probe sets to selectively amplify telomeric repeats and the mitochondrial ND1 gene while excluding nuclear genomic loci. A single-copy reference (SCR) gene enables robust internal normalization for either absolute or relative quantification. A reference DNA standard with certified TL and mtDNA-CN values is included for assay validation and consistency.
The single-tube duplex reaction provides linear quantification across at least five orders of magnitude and is compatible with DNA isolated from cultured cells, tissues, whole blood, saliva, urine, lavage fluids, cerebrospinal fluid (CSF), or scraped cells. Both TL and mtDNA-CN measurements can be generated in under two hours, streamlining high-throughput and time-sensitive workflows.
| Component | Size / Description |
|---|---|
| Probe qPCR Master Mix | 1 mL / 1 tube |
| Primer and Probe Mix | 1 mL / 1 tube |
| Reference DNA | 20 µL / 1 tube |
| Nuclease-free H2O | 1 mL / 1 tube |
Contact our technical support team for questions about compatibility: techsupport@raybiotech.com
The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C. Avoid repeated freeze-thaw cycles.
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Example standard curves of VIC, FAM and JUN amplification.
The RayBio® Human Telomere Length and Mitochondrial DNA Copy Number Dual Quantification Kit is a TaqMan™ probe-based duplex qPCR system with highly specific primer–probe sets to selectively amplify telomeric repeats and the mitochondrial ND1 gene while excluding nuclear genomic loci.
Example standard curves of VIC, FAM and JUN amplification.
